LOCUS       sequence-193056-        8600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric SOX2-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN23
FEATURES             Location/Qualifiers
     source          1..8600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2467..3055
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2520..2540)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2938..2949)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3208..3388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3460..3581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3497..3516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3551..3570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3621..3756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3776..3795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3777..3795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3805..3822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3805..3821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3814..3836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3963..4418
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4050..4069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4260..4281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4444..4548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4549..4617,4618..5409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4767..4786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5580..6168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6069..6088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6322..6339
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6456..6477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6492..6497,6498..6515,6516..6522)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6530..6546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6535..6557
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6554..6570
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6554..6570
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6589..6609
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6591..6609
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6637..6863
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6864..7044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7091..7216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7709..7942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8127..8171
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8438..8555
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat gtacaacatg
     1321 atggagacgg agctgaagcc gccgggcccg cagcaaactt cggggggcgg cggcggcaac
     1381 tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc cggaccgcgt caagcggccc
     1441 atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca agatggccca ggagaacccc
     1501 aagatgcaca actcggagat cagcaagcgc ctgggcgccg agtggaaact tttgtcggag
     1561 acggagaagc ggccgttcat cgacgaggct aagcggctgc gagcgctgca catgaaggag
     1621 cacccggatt ataaataccg gccccggcgg aaaaccaaga cgctcatgaa gaaggataag
     1681 tacacgctgc ccggcgggct gctggccccc ggcggcaata gcatggcgag cggggtcggg
     1741 gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg acagttacgc gcacatgaac
     1801 ggctggagca acggcagcta cagcatgatg caggaccagc tgggctaccc gcagcacccg
     1861 ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc accgctacga cgtgagcgcc
     1921 ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc cacctacagc
     1981 atgtcctact cgcagcaggg cacccctggc atggctcttg gctccatggg ttcggtggtc
     2041 aagtccgagg ccagctccag cccccctgtg gttacctctt cctcccactc cagggcgccc
     2101 tgccaggccg gggacctccg ggacatgatc agcatgtatc tccccggcgc cgaggtgccg
     2161 gaacccgccg cccccagcag acttcacatg tcccagcact accagagcgg cccggtgccc
     2221 ggcacggcca ttaacggcac actgcccctc tcacacatgc acttagacgg cgaggacgtg
     2281 gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat
     2341 tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc
     2401 gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggtga
     2461 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2521 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2581 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2641 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2701 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2761 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2821 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     2881 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2941 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3001 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     3061 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     3121 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     3181 tgctagctcc ggatccggac ctgtactggg tctctctggt tagaccagat ctgagcctgg
     3241 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3301 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3361 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     3421 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     3481 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3541 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     3601 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3721 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     3781 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3841 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3901 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3961 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4021 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4081 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4141 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4201 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4261 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4321 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4381 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     4441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5701 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6001 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6061 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6241 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6301 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6361 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6421 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6481 gcaccccagg ttttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6541 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6601 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6661 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6721 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6781 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6841 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6901 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6961 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7021 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7081 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7141 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7201 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7261 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7321 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7381 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7441 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7501 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7561 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7621 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7681 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7741 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7801 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7861 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7921 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7981 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8041 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8101 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8161 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8221 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8281 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8341 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8401 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     8461 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     8521 aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact agcctcgaga
     8581 agcttgatat cgaattccac
//
