LOCUS       sequence-193061-        8679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, 
            Dokholyan NV, Hahn KM
  TITLE     Engineering extrinsic disorder to control protein activity in living
            cells.
  JOURNAL   Science. 2016 Dec 16;354(6318):1441-1444.
  PUBMED    27980211
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-12-16"; volume: "354(6318)"; pages: "1441-1444"
COMMENT     Sequence Label: pBabe-PI(WT)-Src(WT)-mCherry
FEATURES             Location/Qualifiers
     source          1..8679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     137..156
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             291..1316
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             291..1316
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(1793..1810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1964..2552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2044..2063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2712..3503,3504..3572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3335..3354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3573..3677)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3708..4177
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4242..4441
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4642..5058
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4988..5010
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5026..5042
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    join(5099..5117,5118..5140,5141..5159,5160..5182,5183..5201,
                     5202..5224,5225..5243,5244..5266,5267..5285,5286..5308,
                     5309..5327,5328..5350,5351..5369)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5402..5440
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5437..5461
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5553..5562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6426..6854
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     regulatory      7605..7614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7611..8321
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7756..7774)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8024..8043
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    8335..8351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(8384..8679,1..204)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8419..8442)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
       61 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      121 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      181 tcttcctcat ctccgggcct ttcgacctgc agccaataag cttgccagct ggggcgccct
      241 ctggtaaggt tgggaagccc tgcagatctg atcccggggg gcaatgagat atgaaaaagc
      301 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
      361 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
      421 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
      481 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta
      541 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
      601 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
      661 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
      721 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
      781 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
      841 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
      901 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
      961 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     1021 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     1081 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     1141 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     1201 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     1261 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     1321 gatgccgacc gggatctatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     1381 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     1441 gcatggaaaa atacataact gagaatagag aagttcagat caaggtagga gatccctgag
     1501 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     1561 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     1621 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
     1681 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
     1741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2641 ggatcttcac ctagatcctt ttgcggccgc aaatcaatct aaagtatata tgagtaaact
     2701 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2761 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2821 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2881 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2941 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3001 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3061 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3121 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3181 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3241 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3301 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3361 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3421 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3481 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3541 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3661 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc
     3721 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     3781 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     3841 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     3901 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     3961 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     4021 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     4081 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     4141 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
     4201 gagacccctg cccagggacc accgacccac caccgggagg caagctggcc agcaacttat
     4261 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
     4321 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc
     4381 ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga
     4441 ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag
     4501 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc
     4561 gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
     4621 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
     4681 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     4741 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     4801 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     4861 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     4921 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     4981 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     5041 actccttctc taggcgccgg ccggatccac gaggcccttt cgtcgtcgag tttaccactc
     5101 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     5161 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     5221 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     5281 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     5341 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
     5401 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     5461 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     5521 cctccgcggc cccagatccg taaagtgaat tcgccaccat gggcagcaac aagagcaagc
     5581 ccaaggacgc cagccagcgg cgccgcagcc tggagccctc ggaaaacgtg cacggggcag
     5641 ggggcgcctt cccggcctca cagacaccga gcaagcccgc ctccgccgac ggccaccgcg
     5701 ggcccagcgc cgccttcgtg ccgcccgcgg ccgagcccaa gctcttcgga ggcttcaact
     5761 cctcggacac cgtcacctcc ccgcagaggg cggggcctct ggcaggtggg gtgaccacct
     5821 ttgtggccct ctatgactat gagtcacgga cagagactga cctgtccttc aagaaagggg
     5881 agcggctgca gattgttaac aacacggagg gagactggtg gctggcacac tcgctgagca
     5941 cgggacagac cggttacatc cccagcaact atgtggcgcc ctccgactcc atccaggctg
     6001 aggagtggta ctttggcaag atcactagac gggaatcaga gcggctgctg ctcaacgccg
     6061 agaacccgag agggaccttc ctcgtgaggg agagtgagac cacaaaaggt gcctactgcc
     6121 tctctgtatc cgacttcgac aatgccaagg gcctaaatgt gaaacactac aagatccgca
     6181 agctggacag cggcggtttc tacatcacct cccgcaccca gttcaacagc ctgcagcagc
     6241 tcgtggctta ctactccaaa catgctgatg gcctgtgtca ccgcctcact accgtatgtc
     6301 ccacatccaa gcctcagacc cagggattgg ccaaggatgc gtgggagatc ccccgggagt
     6361 ccctgcggct ggaggtcaag ctgggccagg gttgcttcgg agaggtgtgg atggggacct
     6421 ggaacttggc tactacactt gaacgtattg agaagaactt tgtcattact gacccaagat
     6481 tgccagataa tcccattata ttcgcgtccg atagtttctt gcagttgaca gaatatagcc
     6541 gtgaagaaat tttgggaaga aactgcaggt ttctacaagg tcctgaaact gatcgcgcga
     6601 cagtgagaaa aattagagat gccatagata accaaacaga ggtcactgtt cagctgatta
     6661 attatacaaa gagtggtaaa aagttctgga acctctttca cttgcagcct atgcgagatc
     6721 agaagggaga tgtccagtac tttattgggg ttcagttgga tggaactgag catgtccgag
     6781 atgctgccga gagagaggga gtcatgctga ttaagaaaac tgcagaaaat attgatgagg
     6841 cggcaaaaga acttaccacg agggttgcca tcaaaactct gaagccaggc accatgtccc
     6901 cagaggcctt cctgcaggag gcccaagtca tgaagaaact gaggcacgag aaactggtgc
     6961 agctgtatgc tgtggtgtcg gaagaaccca tttacattgt gacagagtac atgaacaagg
     7021 ggagtctgct ggactttctc aagggggaaa cgggcaaata tttgcggcta ccccagctgg
     7081 tggacatgtc tgctcagatc gcttcaggca tggcctatgt ggagcggatg aactatgtgc
     7141 accgggacct tcgagccgcc aatatcctag taggggagaa cctggtgtgc aaagtggccg
     7201 actttgggtt ggcccggctc atagaagaca acgaatacac agcccggcaa ggtgccaaat
     7261 tccccatcaa gtggaccgcc cctgaagctg ctctgtacgg caggttcacc atcaagtcgg
     7321 atgtgtggtc ctttgggatt ctgctgaccg agctcaccac taagggaaga gtgccctatc
     7381 ctgggatggt gaaccgtgag gttctggacc aggtggagcg gggctaccgg atgccttgtc
     7441 cccccgagtg ccccgagtcc ctgcatgacc ttatgtgcca gtgctggcgg aaggagcccg
     7501 aggagcggcc caccttcgag tacctgcagg ccttcctgga agactacttt acgtccactg
     7561 agccacagta ccagcccggg gagaacctat cggatccacc ggtcgccacc atggtgagca
     7621 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     7681 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     7741 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     7801 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     7861 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     7921 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     7981 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     8041 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     8101 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     8161 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     8221 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     8281 gccgccactc caccggcggc atggacgagc tgtacaagta agtcgagtgt ggaattgtga
     8341 gcggataaca atttgtcgag ggcccctgca ggtcaattct accgggtagg ggaggcgctt
     8401 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     8461 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     8521 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     8581 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     8641 cagatggaca gcaccgctga gcaatggaag cgggtaggc
//
