LOCUS       sequence-193130-       10770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 
            mutant W161H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-myc-CXCR4-W161H
FEATURES             Location/Qualifiers
     source          1..10770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(823..841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        909..1013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1014..1082,1083..1874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1232..1251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2045..2633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2534..2553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3064..3209
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3252..4289
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4287..4306)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4331..4379
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5033..5412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5413..5616
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5567..5587
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5613..5637
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5719..5748
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6487..6498
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6845..6979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6869..6888)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6923..6942
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7122..7144)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7704..9493
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgnncccct cctgcccctc
       61 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc
      121 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga
      181 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc
      241 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc
      301 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag
      361 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt
      421 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc
      481 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc
      541 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca
      601 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg
      661 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac
      721 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc
      781 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
      841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     1561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt
     2701 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
     2761 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
     2821 agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     2881 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     2941 tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat ccccgtggcc
     3001 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
     3061 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
     3121 tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
     3181 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg
     3241 ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     3301 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     3361 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     3421 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     3481 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     3541 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     3601 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     3661 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     3721 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     3781 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     3841 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     3901 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     3961 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     4021 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     4081 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     4141 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     4201 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     4261 cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     4321 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     4381 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4441 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4501 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg
     4561 ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc
     4621 gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc
     4681 ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac
     4741 acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt
     4801 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     4861 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     4921 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     4981 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     5041 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5101 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5161 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5221 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5281 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5341 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     5401 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     5461 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     5521 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     5581 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct
     5641 agaagctggg taccagctgc tagcgccacc atgaagacca tcatcgccct gagctacatc
     5701 ttctgcctgg tgttcgccga gcagaaattg atcagcgagg aggacctggg cagccctgga
     5761 ggcgccgagg ggatcagtat atacacttca gataactaca ccgaggaaat gggctcaggg
     5821 gactatgact ccatgaagga accctgtttc cgtgaagaaa atgctaattt caataaaatc
     5881 ttcctgccca ccatctactc catcatcttc ttaactggca ttgtgggcaa tggattggtc
     5941 atcctggtca tgggttacca gaagaaactg agaagcatga cggacaagta caggctgcac
     6001 ctgtcagtgg ccgacctcct ctttgtcatc acgcttccct tctgggcagt tgatgccgtg
     6061 gcaaactggt actttgggaa cttcctatgc aaggcagtcc atgtcatcta cacagtcaac
     6121 ctctacagca gtgtcctcat cctggccttc atcagtctgg accgctacct ggccatcgtc
     6181 cacgccacca acagtcagag gccaaggaag ctgttggctg aaaaggtggt ctatgttggc
     6241 gtccacatcc ctgccctcct gctgactatt cccgacttca tctttgccaa cgtcagtgag
     6301 gcagatgaca gatatatctg tgaccgcttc taccccaatg acttgtgggt ggttgtgttc
     6361 cagtttcagc acatcatggt tggccttatc ctgcctggta ttgtcatcct gtcctgctat
     6421 tgcattatca tctccaagct gtcacactcc aagggccacc agaagcgcaa ggccctcaag
     6481 accacagtca tcctcatcct ggctttcttc gcctgttggc tgccttacta cattgggatc
     6541 agcatcgact ccttcatcct cctggaaatc atcaagcaag ggtgtgagtt tgagaacact
     6601 gtgcacaagt ggatttccat caccgaggcc ctagctttct tccactgttg tctgaacccc
     6661 atcctctatg ctttccttgg agccaaattt aaaacctctg cccagcacgc actcacctct
     6721 gtgagcagag ggtccagcct caagatcctc tccaaaggaa agcgaggtgg acattcatct
     6781 gtttccactg agtctgagtc ttcaagtttt cactccagct aatagctcga ggccggcaag
     6841 gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6901 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6961 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     7021 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     7081 tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7141 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7201 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga
     7261 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     7321 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     7381 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     7441 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     7501 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     7561 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     7621 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     7681 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     7741 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     7801 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     7861 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     7921 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     7981 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     8041 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     8101 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     8161 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     8221 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     8281 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     8341 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     8401 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     8461 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     8521 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     8581 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     8641 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     8701 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     8761 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     8821 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     8881 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     8941 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     9001 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     9061 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     9121 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     9181 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     9241 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     9301 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     9361 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     9421 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     9481 tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc
     9541 cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt
     9601 gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga
     9661 ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta
     9721 ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct
     9781 cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct
     9841 ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga
     9901 ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg
     9961 ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt
    10021 tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg
    10081 ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata
    10141 gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac
    10201 acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga
    10261 gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc
    10321 tttccaaacc accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg
    10381 cttgggcctt ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca
    10441 ggctcaccat ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg
    10501 tggaaaaatg gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac
    10561 tgactactgg gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt
    10621 tcacgacttc cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct
    10681 cgaccccggc ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct
    10741 ccacctcctg ctcctgcccc tcctgctcct
//
