LOCUS       sequence-193175-       13190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The promoter of AAEL006511 drive expression of Streptococcus 
            pyogenes Cas9 gene in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Li M, Bui M, Yang T, Bowman CS, White BJ, Akbari OS
  TITLE     Germline Cas9 expression yields highly efficient genome engineering 
            in a major worldwide disease vector, Aedes aegypti.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Nov 14. pii: 201711538. doi: 
            10.1073/pnas.1711538114.
  PUBMED    29138316
REFERENCE   2  (bases 1 to 13190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Nov 14. pii: 201711538. doi: 
            10.1073/pnas.1711538114."
COMMENT     Sequence Label: AAEL006511-Cas9
FEATURES             Location/Qualifiers
     source          1..13190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(504..557)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(558..605)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(606..4706)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(606..4706)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4731..4751)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4758..4823)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    5456..5525
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   6559..6621
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7325..7342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7325..7341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7334..7356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7550..7569)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7669..7691
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7729..7747)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7815..7919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7920..7988,7989..8780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8138..8157)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8951..9539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9440..9459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9693..9710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9827..9848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9863..9868,9869..9886,9887..9893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9901..9917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9906..9928
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9925..9941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9925..9941
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   10122..10156
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    10824..10945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10835..10854)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10889..10908
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(11059..11733,11734..11736,11737..11739))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(11121..11144)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(11319..11338)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11536..11556
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     CDS             complement(join(12974..13190,1..497,498..500,501..503))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13019..13040)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
       61 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      121 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      181 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      241 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      301 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgct ggtggtgcag
      361 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      421 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      481 agctcctcgc ccttgctcac catagggccg ggattctcct ccacgtcacc gcatgttaga
      541 agacttcctc tgccctcctt tttctttttt gcctggccgg cctttttcgt ggccgccggc
      601 cttttgtcgc ctcccagctg agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc
      661 tggtggatca gggtggcgtc cagcacctct ttggtgctgg tgtacctctt ccggtcgatg
      721 gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat tggtcagggt aaacaggtgg
      781 atgatattct cggcctgctc tctgatgggc ttatcccggt gcttgttgta ggcggacagc
      841 actttgtcca gattagcgtc ggccaggatc actctcttgg agaactcgct gatctgctcg
      901 atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg ctcattatcc
      961 tcgggggagc ccttcagctt ctcatagtgg ctggccaggt acaggaagtt cacatatttg
     1021 gagggcaggg ccagttcgtt tcccttctgc agttcgccgg cagaggccag cattctcttc
     1081 cggccgtttt ccagctcgaa cagggagtac ttaggcagct tgatgatcag gtcctttttc
     1141 acttctttgt agcccttggc ttccagaaag tcgatgggat tcttctcgaa gctgcttctt
     1201 tccatgatgg tgatccccag cagctctttc acactcttca gtttcttgga cttgcccttt
     1261 tccactttgg ccaccaccag cacagaatag gccacggtgg ggctgtcgaa gccgccgtac
     1321 ttcttagggt cccagtcctt ctttctggcg atcagcttat cgctgttcct cttgggcagg
     1381 atagactctt tgctgaagcc gcctgtctgc acctcggtct ttttcacgat attcacttgg
     1441 ggcatgctca gcactttccg cacggtggca aaatcccggc ccttatccca cacgatctcc
     1501 ccggtttcgc cgtttgtctc gatcagaggc cgcttccgga tctcgccgtt ggccagggta
     1561 atctcggtct tgaaaaagtt catgatgttg ctgtagaaga agtacttggc ggtagccttg
     1621 ccgatttcct gctcgctctt ggcgatcatc ttccgcacgt cgtacacctt gtagtcgccg
     1681 tacacgaact cgctttccag cttagggtac tttttgatca gggcggttcc cacgacggcg
     1741 ttcaggtagg cgtcgtgggc gtggtggtag ttgttgatct cgcgcacttt gtaaaactgg
     1801 aaatccttcc ggaaatcgga caccagcttg gacttcaggg tgatcacttt cacttcccgg
     1861 atcagcttgt cattctcgtc gtacttagtg ttcatccggg agtccaggat ctgtgccacg
     1921 tgctttgtga tctgccgggt ttccaccagc tgtctcttga tgaagccggc cttatccagt
     1981 tcgctcaggc cgcctctctc ggccttggtc agattgtcga actttctctg ggtaatcagc
     2041 ttggcgttca gcagctgccg ccagtagttc ttcatcttct tcacgacctc ttcggagggc
     2101 acgttgtcgc tcttgccccg gttcttgtcg cttctggtca gcaccttgtt gtcgatggag
     2161 tcgtccttca gaaagctctg aggcacgata tggtccacat cgtagtcgga cagccggttg
     2221 atgtccagtt cctggtccac gtacatatcc cgcccattct gcaggtagta caggtacagc
     2281 ttctcgttct gcagctgggt gttttccacg gggtgttctt tcaggatctg gctgcccagc
     2341 tctttgatgc cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg
     2401 gtggtctggt tctctctggc catttcgatc acgatgttct cgggcttgtg ccggcccatc
     2461 actttcacga gctcgtccac caccttcact gtctgcagga tgcccttctt aatggcgggg
     2521 ctgccggcca gattggcaat gtgctcgtgc aggctatcgc cctggccgga cacctgggct
     2581 ttctggatgt cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg
     2641 ttggcgaagc cgtcggactt caggaaatcc aggattgtct tgccggactg cttgtcccgg
     2701 atgccgttga tcagcttccg gctcagcctg ccccagccgg tgtatctccg ccgcttcagc
     2761 tgcttcatca ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc ctcgatcatc
     2821 tctctgtcct caaacagtgt cagggtcagc acgatatctt ccagaatgtc ctcgttttcc
     2881 tcattgtcca ggaagtcctt gtccttgata attttcagca gatcgtggta tgtgcccagg
     2941 gaggcgttga accgatcttc cacgccggag atttccacgg agtcgaagca ctcgattttc
     3001 ttgaagtagt cctctttcag ctgcttcacg gtcactttcc ggttggtctt gaacagcagg
     3061 tccacgatgg cctttttctg ctcgccgctc aggaaggcgg gctttctcat tccctcggtc
     3121 acgtatttca ctttggtcag ctcgttatac acggtgaagt actcgtacag caggctgtgc
     3181 ttgggcagca ccttctcgtt gggcaggttc ttatcgaagt tggtcatccg ctcgatgaag
     3241 ctctgggcgg aagcgccctt gtccaccact tcctcgaagt tccagggggt gatggtttcc
     3301 tcgctctttc tggtcatcca ggcgaatctg ctgtttcccc tggccagagg gcccacgtag
     3361 taggggatgc ggaaggtcag gatcttctcg atcttttccc ggttgtcctt caggaatggg
     3421 taaaaatctt cctgccgccg cagaatggcg tgcagctctc ccaggtggat ctggtggggg
     3481 atgctgccgt tgtcgaaggt ccgctgcttc cgcagcaggt cctctctgtt cagcttcacg
     3541 agcagttcct cggtgccgtc catcttttcc aggatgggct tgatgaactt gtagaactct
     3601 tcctggctgg ctccgccgtc aatgtagccg gcgtagccgt tcttgctctg gtcgaagaaa
     3661 atctctttgt acttctcagg cagctgctgc cgcacgagag ctttcagcag ggtcaggtcc
     3721 tggtggtgct cgtcgtatct cttgatcata gaggcgctca ggggggcctt ggtgatctcg
     3781 gtgttcactc tcaggatgtc gctcagcagg atggcgtcgg acaggttctt ggcggccaga
     3841 aacaggtcgg cgtactggtc gccgatctgg gccagcaggt tgtccaggtc gtcgtcgtag
     3901 gtgtccttgc tcagctgcag tttggcatcc tcggccaggt cgaagttgct cttgaagttg
     3961 ggggtcaggc ccaggctcag ggcaatcagg tttccgaaca ggccattctt cttctcgccg
     4021 ggcagctggg cgatcagatt ttccagccgt ctgctcttgc tcagtctggc agacaggatg
     4081 gccttggcgt ccacgccgct ggcgttgatg gggttttcct cgaacagctg gttgtaggtc
     4141 tgcaccagct ggatgaacag cttgtccacg tcgctgttgt cggggttcag gtcgccctcg
     4201 atcaggaagt ggccccggaa cttgatcatg tgggccaggg ccagatagat cagccgcagg
     4261 tcggccttgt cggtgctgtc caccagtttc tttctcaggt ggtagatggt ggggtacttc
     4321 tcgtggtagg ccacctcgtc cacgatgttg ccgaagatgg ggtgccgctc gtgcttctta
     4381 tcctcttcca ccaggaagga ctcttccagt ctgtggaaga agctgtcgtc caccttggcc
     4441 atctcgttgc tgaagatctc ttgcagatag cagatccggt tcttccgtct ggtgtatctt
     4501 cttctggcgg ttctcttcag ccgggtggcc tcggctgttt cgccgctgtc gaacagcagg
     4561 gctccgatca ggttcttctt gatgctgtgc cggtcggtgt tgcccagcac cttgaatttc
     4621 ttgctgggca ccttgtactc gtcggtgatc acggcccagc ccacagagtt ggtgccgatg
     4681 tccaggccga tgctgtactt cttgtcggct gctgggactc cgtggatacc gaccttccgc
     4741 ttcttctttg gggccatctt atcgtcatcg tctttgtaat caatatcatg atccttgtag
     4801 tctccgtcgt ggtccttata gtccatctcg agcttggatc agtctgtgga aaagtcgggg
     4861 agaaaacacc aaaaattaac gaactggaca ataacatcgg aattggacgg agacgaaacc
     4921 acattgaggt tagaatgcat caactacaac aaatcgaata aactacgaat aatgtaggta
     4981 ttttagcaat atttcacaat ttctaacttg atctatcaat gatttagatt gctaacaacg
     5041 aaaaaccacg aattatcact aaaaatccac gaaaaacgac aaaaattaaa cttactcttt
     5101 cccaacgtgt ctcttccgag atgtgtttcg gtgaaaaaga gcttcaagtc agtggagctg
     5161 tcaaaactga atttcagttc agtggccggc gccgaatgga tacccggtca tgaaatgcaa
     5221 tcaaattctg tgcacgcatg caaggttcac gctgcggccg cgtcgacatg cccgccgtga
     5281 ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc
     5341 ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc
     5401 gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg gaccagatgg
     5461 gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg caccgcggct
     5521 tcgagaccgt gacctacatc gtcgacctag gaccccgggc gagctcgaat taaccattgt
     5581 gggaaccgtg cgatcaaaca aacgcgagat accggaagta ctgaaaaaca gtcgctccag
     5641 gccagtggga acatcgatgt tttgttttga cggacccctt actctcgtct catataaacc
     5701 gaagccagct aagatggtat acttattatc atcttgtgat gaggatgctt ctatcaacga
     5761 aagtaccggt aaaccgcaaa tggttatgta ttataatcaa actaaaggcg gagtggacac
     5821 gctagaccaa atgtgttctg tgatgacctg cagtaggaag acgaataggt ggcctatggc
     5881 attattgtac ggaatgataa acattgcctg cataaattct tttattatat acagccataa
     5941 tgtcagtagc aagggagaaa aggttcaaag tcgcaaaaaa tttatgagaa acctttacat
     6001 gagcctgacg tcatcgttta tgcgtaagcg tttagaagct cctactttga agagatattt
     6061 gcgcgataat atctctaata ttttgccaaa tgaagtgcct ggtacatcag atgacagtac
     6121 tgaagagcca gtaatgaaaa aacgtactta ctgtacttac tgcccctcta aaataaggcg
     6181 aaaggcaaat gcatcgtgca aaaaatgcaa aaaagttatt tgtcgagagc ataatattga
     6241 tatgtgccaa agttgtttct gactgactaa taagtataat ttgtttctat tatgtataag
     6301 ttaagctaat tacttatttt ataatacaac atgactgttt ttaaagtaca aaataagttt
     6361 atttttgtaa aagagagaat gtttaaaagt tttgttactt tatagaagaa attttgagtt
     6421 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     6481 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     6541 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
     6601 aatatgatta tctttctagg gttaaataat agtttctaat ttttttatta ttcagcctgc
     6661 tgtcgtgaat accgtatatc tcaacgctgt ctgtgagatt gtcgtattct agccttttta
     6721 gtttttcgct catcgacttg atattgtccg acacattttc gtcgatttgc gttttgatca
     6781 aagacttgag cagagacacg ttaatcaact gttcaaattg atccatatta acgatatcaa
     6841 cccgatgcgt atatggtgcg taaaatatat tttttaaccc tcttatactt tgcactctgc
     6901 gttaatacgc gttcgtgtac agacgtaatc atgttttctt ttttggataa aactcctact
     6961 gagtttgacc tcatattaga ccctcacaag ttgcaaaacg tggcattttt taccaatgaa
     7021 gaatttaaag ttattttaaa aaatttcatc acagatttaa agaagaacca aaaattaaat
     7081 tatttcaaca gtttaatcga ccagttaatc aacgtgtaca cagacgcgtc ggcaaaaaac
     7141 acgcagcccg acgtgttggc taaaattatt aaatcaactt gtgttatagt cacggatttg
     7201 ccgtccaacg tgttcctcaa aaagttgaag accaacaagt ttacggacac tattaattat
     7261 ttgattttgc cccacttcat tttgtgggat cacaattttg ttatattttt aaacaaagct
     7321 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7381 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7441 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     7501 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7561 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7621 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7681 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7741 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7801 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7861 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7921 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7981 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8041 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8101 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8161 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8221 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8281 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8341 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8401 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8461 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8521 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8581 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8641 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8701 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8761 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8821 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8881 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8941 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9001 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9061 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9121 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9181 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9241 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9301 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9361 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9421 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9481 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9541 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9601 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9661 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9721 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9781 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9841 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9901 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattcgagct
     9961 cggtacccgg ggatcctcta gagtcgacgc tcgcgcgact tggtttgcca ttctttagcg
    10021 cgcgtcgcgt cacacagctt ggccacaatg tggtttttgt caaacgaaga ttctatgacg
    10081 tgtttaaagt ttaggtcgag taaagcgcaa atctttttta accctagaaa gatagtctgc
    10141 gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg
    10201 tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca
    10261 atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga
    10321 ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt
    10381 tctacttacg tgataactta ttatatatat attttcttgt tatagatatc gtgactaata
    10441 tataataaaa tgggtagttc tttagacgat gagcatatcc tctctgctct tctgcaaagc
    10501 gatgacgagc ttgttggtga ggattctgac agtgaaatat cagatcacgt aagtgaagat
    10561 gacgtccaga gcgatacaga agaagcgttt atagatgagg tacatgaagt gcagccaacg
    10621 tcaagcggta gtgaaatatt agacgaacaa aatgttattg aacaaccagg ttcttcattg
    10681 gcttctaaca gaatcttgac cttgccacag aggactatta gaggtaagaa taaacattgt
    10741 tggtcaactt caaagtccac gaggcgtagc cgagtctctg cactgaacat tgtcagatcc
    10801 gagatcggcc ggcctagaag ctttaagata cattgatgag tttggacaaa ccacaactag
    10861 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
    10921 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
    10981 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
    11041 tgattatgat ctagagtcct acaggaacag gtggtggcgg ccctcggtgc gctcgtactg
    11101 ctccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggagt ccacgtagta
    11161 gtagccgggc agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta
    11221 gtggccgccg tccttcagct tcagggcctt gtggatctcg cccttcagca cgccgtcgcg
    11281 ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc
    11341 gtcggagggg aagttcacgc cgatgaactt caccttgtag atgaagcagc cgtcctgcag
    11401 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
    11461 cttgaagccc tcggggaagg acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac
    11521 gtacaccttg gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg
    11581 gccgcccttg gtcaccttca gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc
    11641 ctcgccctcg atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg
    11701 catgaactcc ttgatgacgt tcttggagga gcgcaccatc accagagaca ggttgcggcg
    11761 gcggttggat ggcgtgggcg cgttggcgtt gttggaccgg ctcatgttgt gtcgctgtaa
    11821 cagatgctgt tcaactgtgt ttaccagatc gttgcgggct gtatttatag gcgcgataag
    11881 cgggacgggc gcctcgtgtc cggtcacgcg catgagataa cgcgcggctg atatggaggc
    11941 gcgtcctgtt ccgataagga gttgcgtccg gctgcggtta gcaacacagg aagctggcgt
    12001 cctgtcacga taagacaaca ctcgtccggt ccgataatgt gattcgtacg tgacaggacg
    12061 cgacccgata aggccggcct acgtgactgc cgacacgtac ttttttgcac tgcaaaaagg
    12121 ttcaatgtgt ggtagtgtat ttggagcgta tacaacggtg tagactattt atgtaaaata
    12181 gtctacgaaa cgtagagttt gtactatgta tgggcccgcg tgcaaaagcg tgtttttttg
    12241 cagtgcaaaa aagttggtgg tggggaggcc accgagtatg ggcgcgcccc ggccgttaac
    12301 tcgaatcgct atccaagcca gctccattgt cggcatcgtg ctcattctat tgacgctggc
    12361 agatttggtt ttggcgctat gggacccgtt cggttacaac aacatgtttc cgcgcgagtt
    12421 tcccgacgac atgtcgcgca cgttcctgac tgcgtacttt gagagtttcg acaacaccac
    12481 gtccagagaa atcatagagt ttatgcccga gttcttttcg gaaatggtcg aaacggacga
    12541 tgacgccacg tttgaatctc tatttcattt attagattat gtggcatctt tagaagttaa
    12601 ttccgacggc caaatgttaa acttggagga gggtgatgaa attgaggatt ttgacgaatc
    12661 tactttggtg gggcaagcgt tagccactag ctcgctatac actcgcatgg agtttatgca
    12721 gtacacgttt aggcaaaaca cactattgtc tatgaacaaa gaaaacaaca attttaatca
    12781 aataatcatg ggtttatttg caacaaacac aattgtggcg tttacagcat ttgttataca
    12841 cacagaactc atatttttta tatttttcgt aatcttccta atgatcacat tttattacat
    12901 aatcaaagaa tcgtacgaat attataaaac aattgatttg ttattttaaa aacgattcat
    12961 tctagttaat taattacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
    13021 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
    13081 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
    13141 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
//
