LOCUS       sequence-19318-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf241
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..442
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             452..469
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(492..509)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    498..705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtacccttca ccgaattcca gctgaccacc atgtacaact catcaattca tacgccggaa
       61 tatgatgtta taatccacgt aattgagcat ctcaagcatc acaaacaatg cgttcagact
      121 gttactagtg ggatggtctt cacctcccca gtaagtagca gcatatgcac aaagtccgat
      181 gatggccgaa atctctctga tggttttttg ctcatccggt atataacaac ggatgatttc
      241 tgcacaatct ttgacatcat cccacggcat attttttacc agctcgcgaa tgtcgatgaa
      301 cactaagcaa ttcccgggga tccgaataaa gggtcaagac aattctgcag atatccagca
      361 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
      421 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
      481 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      541 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      601 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      661 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcg
//
