LOCUS       sequence-193209-        8730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Sun Q, Zhang J, Fan W, Wong KN, Ding X, Chen S, Zhong Q
  TITLE     The RUN domain of rubicon is important for hvps34 binding, lipid 
            kinase inhibition, and autophagy suppression.
  JOURNAL   J Biol Chem. 2011 Jan 7. 286(1):185-91.
  PUBMED    21062745
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jan 7. 286(1):185-91."
COMMENT     Sequence Label: EGFP-Rubicon
FEATURES             Location/Qualifiers
     source          1..8730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        110..489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        490..693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     644..664
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    695..713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    716..734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     744..768
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(859..861,862..864,865..1575)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(859..861,862..864,865..1575)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(904..925)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1165..1184)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1512..1533
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4507..4572
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4613..4630)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4619..4843
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4889..5317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4976..4995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5186..5207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5326..5346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5331..5660
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5511..5646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5573..5592
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5708..5755
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5774..6148
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6278..6399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6315..6334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6369..6388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6448..6464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6448..6464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6461..6483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6472..6488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6496..6502,6503..6520,6521..6526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6541..6562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6679..6696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6850..7438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6930..6949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7609..8400,8401..8469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8232..8251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8470..8574)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8649..8668)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
       61 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      121 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      181 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      361 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      421 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      481 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      541 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      601 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      661 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
      721 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      781 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
      841 gcgtttaaac ttaagcttat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      901 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      961 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1021 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1081 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1141 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1201 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1261 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1321 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1381 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1441 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1501 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1561 gacgagctgt acaagggatc catgcggccg gagggcgcgg gaatggagct cggaggcggc
     1621 gaggagcgcc tgcctgagga gagcaggagg gagcactggc agttgctggg taatttgaag
     1681 acgacggtgg agggtttggt atcaaccaac agccccaacg tctggtctaa gtatggtggc
     1741 ttggagcggc tttgcaggga catgcagagc atcctctatc acgggcttat ccgtgaccag
     1801 gcgtgccgcc gccagacgga ttactggcag ttcgtgaaag acatccggtg gctcagtccc
     1861 cactcagccc ttcacgtgga gaagttcatc agcgtgcacg agaacgacca gagcagtgct
     1921 gatggtgcca gtgaacgtgc tgttgccgag ctgtggctgc agcacagcct gcagtaccac
     1981 tgcctctcag cccagctccg gcccctgctc ggggatagac agtatatcag aaaattctac
     2041 acagatgctg ccttcctgct aagtgacgct catgtcacgg ccatgctgca gtgcctggaa
     2101 gccgtcgagc aaaataatcc ccgcctcctg gctcagatcg atgcgtccat gtttgccaga
     2161 aagcacgaga gcccgctcct ggtgacaaag agccagagcc tgacagccct gcccagttcc
     2221 acatacaccc ctccaaacag ctatgctcag cattcctact ttgggtcctt ctctagcctc
     2281 caccaatccg tgcccaacaa tggctcagag agaagatcta cttcctttcc actctctggc
     2341 cctccccgga aacctcaaga aagcagaggg cacgtctcac cagcagagga tcaaaccatc
     2401 caagcccccc cagtttcagt ctctgcacta gccagggatt cccctttgac cccaaatgaa
     2461 atgagctcca gtactctgac cagccccata gaggcatcct gggtcagcag ccagaatgat
     2521 tccccaggtg atgccagtga ggggcctgag tacctggcca ttggcaactt ggacccccga
     2581 ggccggactg ccagctgtca gagtcacagc agcaatgccg agagcagcag ttccaatttg
     2641 ttctcctcca gcagctccca gaagccagat tctgctgcct cttccttagg ggaccaggaa
     2701 ggaggtgggg agagccagct gtccagtgtc ctccgcaggt ccagcttctc agaggggcag
     2761 acactcactg tcaccagtgg ggcaaagaaa agccacattc gctcccattc ggataccagc
     2821 attgcctcca ggggagctcc agaatcctgc aatgataagg cgaagttgag aggccctttg
     2881 ccctactctg gtcaaagcag tgaagtcagc acacccagct ctctgtacat ggaatatgaa
     2941 ggtggtcggt acctgtgctc aggggaaggc atgttccgaa gaccatcaga aggacagtcc
     3001 ctcatcagct acctctctga gcaagacttc ggcagctgtg ccgacctgga aaaggagaat
     3061 gcccacttca gcatctcaga gtccttaatt gctgccatcg agctaatgaa gtgcaacatg
     3121 atgagccagt gcctagagga ggaggaagtg gaagaggaag acagtgatag agagatccag
     3181 gagctgaagc agaagatccg ccttcggcgc cagcaaatcc gcaccaagaa cctgctcccc
     3241 atgtaccagg aggctgagca cggaagcttt cgggtcacct ccagcagctc ccagttcagc
     3301 tcacgtgatt cggcacagct ctctgactct ggctctgctg atgaggttga tgaatttgaa
     3361 atccaagatg ctgacatcag aaggaacaca gcctcaagca gcaaatcctt cgtttcctcc
     3421 cagtccttct cccactgctt cctgcactcc acgtctgctg aggcggtggc catggggctc
     3481 ctgaagcagt ttgaggggat gcagcttcca gccgcctcgg agctggagtg gcttgtcccg
     3541 gagcatgatg cccctcagaa gctcctgccc attcctgact cactgcccat ctcaccggat
     3601 gacgggcagc acgctgacat ctacaagctg cggattcgtg ttcgtggcaa cttggagtgg
     3661 gccccgcccc ggcctcagat aatttttaat gttcatccag ccccaacgag gaaaattgcc
     3721 gtggccaagc agaattaccg ctgtgcagga tgtggcatcc ggactgaccc tgattacatc
     3781 aagcgactgc ggtactgtga gtacctgggc aagtacttct gccagtgctg ccacgagaat
     3841 gcccagatgg ccatccccag ccgggttctg cgcaagtggg acttcagcaa gtactacgtc
     3901 agcaacttct ccaaggacct gctcattaag atctggaatg atcctctctt caacgtgcag
     3961 gacataaaca gtgccctcta taggaaggtc aagctgctca atcaagtccg gctgctgcgg
     4021 gtccagctgt gtcacatgaa gaacatgttc aagacttgcc gactggccaa ggagcttctg
     4081 gattcctttg acacagtccc aggccacctg acagaggacc tccacctgta ctcactgaat
     4141 gacctgactg cgaccaggaa gggggagctg gggccccggc ttgctgagct caccagggca
     4201 ggggctaccc atgtggagag atgcatgctc tgccaagcca aaggcttcat ctgtgagttc
     4261 tgtcagaatg aggatgacat catctttccc tttgagctcc ataagtgccg gacctgtgaa
     4321 gagtgtaaag cgtgttacca taaagcctgc ttcaagtctg gaagctgtcc gcgctgcgag
     4381 cggctgcagg cccggcggga ggcactggcc aggcagagcc tggagtctta cctgtcagac
     4441 tacgaggagg agcccgcgga agcgctggcc ctggaagccg ccgtcctgga ggccaccggc
     4501 ggccgcgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4561 gacgatgaca agtgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     4621 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4681 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4741 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4801 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4861 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4921 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4981 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5041 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5101 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5161 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5221 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5281 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5341 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5401 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5461 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5521 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5581 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5641 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5701 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     5761 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     5821 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     5881 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     5941 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     6001 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     6061 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     6121 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     6181 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6241 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6421 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8581 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8641 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8701 gtgttggagg tcgctgagta gtgcgcgagc
//
