LOCUS       sequence-19321-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf249
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             342..383
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             393..410
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(433..450)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    439..663
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgagctcgg atccagtacc cttcaccgaa ttccagctga ccaccattga gatggattcg
       61 ctgagaccgg tagtggtcgt taactggata cagattaatt tccacatcga tatagttaaa
      121 ggtattactg ggtacgggtt cgcatttatc tgcggaagag acggtgtgag aatatgttcc
      181 gagaccacac ggagaacaga tgacgtctcc ggatactccg tatcctattc cacattttgt
      241 ttgggaagca attcccgggg atccgaataa agggtcaaga caattctgca gatatccagc
      301 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
      361 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa
      421 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
      481 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
      541 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
      601 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
      661 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
      721 gcggcgcatt aagcgcggcg ggtgtgggtg gtacgcgcag cgtgaccgct acacttgcca
      781 gcgccctagc gcccgctcct t
//
