LOCUS       sequence-193263-        8972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric SOX2-repressor fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN25
FEATURES             Location/Qualifiers
     source          1..8972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2839..3427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2892..2912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3310..3321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3580..3760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3832..3953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3869..3888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3923..3942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3993..4128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4148..4167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4149..4167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4177..4194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4177..4193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4186..4208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4335..4790
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4422..4441)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4632..4653
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4816..4920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4921..4989,4990..5781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5139..5158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5952..6540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6441..6460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6694..6711
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6828..6849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6864..6869,6870..6887,6888..6894)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6902..6918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6907..6929
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6926..6942
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6926..6942
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6961..6981
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6963..6981
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7009..7235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7236..7416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7463..7588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8081..8314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8499..8543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8810..8927
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat gtacaacatg
     1321 atggagacgg agctgaagcc gccgggcccg cagcaaactt cggggggcgg cggcggcaac
     1381 tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc cggaccgcgt caagcggccc
     1441 atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca agatggccca ggagaacccc
     1501 aagatgcaca actcggagat cagcaagcgc ctgggcgccg agtggaaact tttgtcggag
     1561 acggagaagc ggccgttcat cgacgaggct aagcggctgc gagcgctgca catgaaggag
     1621 cacccggatt ataaataccg gccccggcgg aaaaccaaga cgctcatgaa gaaggataag
     1681 tacacgctgc ccggcgggct gctggccccc ggcggcaata gcatggcgag cggggtcggg
     1741 gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg acagttacgc gcacatgaac
     1801 ggctggagca acggcagcta cagcatgatg caggaccagc tgggctaccc gcagcacccg
     1861 ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc accgctacga cgtgagcgcc
     1921 ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc cacctacagc
     1981 atgtcctact cgcagcaggg cacccctggc atggctcttg gctccatggg ttcggtggtc
     2041 aagtccgagg ccagctccag cccccctgtg gttacctctt cctcccactc cagggcgccc
     2101 tgccaggccg gggacctccg ggacatgatc agcatgtatc tccccggcgc cgaggtgccg
     2161 gaacccgccg cccccagcag acttcacatg tcccagcact accagagcgg cccggtgccc
     2221 ggcacggcca ttaacggcac actgcccctc tcacacatgg gaaagaaaac caagcggaca
     2281 gctgacagtt cttcttcaga ggatgaggag gagtatgtcg tggagaaggt gctagacagg
     2341 cgcgtggtta agggacaagt ggaatatcta ctgaagtgga aaggcttttc tgaggagcac
     2401 aatacttggg aacctgagaa aaacttggat tgccctgagc taatttctga atttatgaaa
     2461 aagtataaga agatgaagga gggtgaaaat aataaaccca gggagaagtc agaaagtaac
     2521 aagaggaaat ccaatttctc aaacagtgcc gatgacatca aatctaaaaa aaagagagag
     2581 cagagcaatg atatcgctcg gggctttgag agaggactgg aaccagaaaa gatcattggg
     2641 gcaacagatt cctgtggtga tttaatgttc ctaatgaaat ggaaagacac agatgaagct
     2701 gacctggttc ttgcaaaaga agctaatgtg aaatgtccac aaattgtgat agcattttat
     2761 gaagagagac tgacatggca tgcatatcct gaggatgcgg aaaacaaaga gaaagaaaca
     2821 gcaaagagct aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3241 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3361 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3421 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     3481 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3541 gacaagatat cctgctagct ccggatccgg acctgtactg ggtctctctg gttagaccag
     3601 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3661 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3721 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     3781 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3961 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4021 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4081 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     4141 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     4201 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4261 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4321 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4381 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4441 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4501 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4561 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4621 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4681 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4741 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4801 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4861 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4921 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4981 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5041 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5101 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5161 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5221 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5281 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5341 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5401 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5461 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5521 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5581 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5641 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5701 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5761 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5821 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5881 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6061 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6121 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6601 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6661 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6721 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6781 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6841 ctcactcatt aggcacccca ggttttacac tttatgcttc cggctcgtat gttgtgtgga
     6901 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6961 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     7021 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     7081 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     7141 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     7201 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     7261 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7321 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7381 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7441 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7501 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7561 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7621 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7681 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7741 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7801 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7861 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7921 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7981 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8041 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8101 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8161 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8221 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8281 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8341 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8401 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8461 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8521 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8581 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8641 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8701 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8761 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     8821 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     8881 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc gatcacgaga
     8941 ctagcctcga gaagcttgat atcgaattcc ac
//
