LOCUS       sequence-193279-        6384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 1-450 of HCF-1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Wilson AC, Freiman RN, Goto H, Nishimoto T, Herr W
  TITLE     VP16 targets an amino-terminal domain of HCF involved in cell cycle 
            progression.
  JOURNAL   Mol Cell Biol. 1997 Oct;17(10):6139-46.
  PUBMED    9315674
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-10"; volume: "17(10)"; pages: "6139-46"
COMMENT     Sequence Label: pCGN-HCF-1-N450
FEATURES             Location/Qualifiers
     source          1..6384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          2082..2654
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2786..2805)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2851..2906
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2905..2924)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3260..3395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3501..3518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3672..4260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3752..3771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4431..5222,5223..5291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5054..5073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5292..5396)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5464..5482
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5520..5542)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5642..5661
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5728..6183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5865..5886)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6077..6096
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6309..6331
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6323..6340
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6324..6340
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6347..6366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6347..6365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(6384,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agagcttcgg
      721 ccgtgtcgcc cgccaacttg ccagcggtgc ttctgcagcc ccgctggaag cgagtggtgg
      781 gctggtcggg tccggtgcca cggccccgcc acggccaccg cgccgtggcc atcaaggagc
      841 tcatcgtggt gtttggcggc ggcaacgagg gaatagtgga cgaactgcac gtgtacaaca
      901 cggcaaccaa ccagtggttc atcccagccg tgagggggga cattccccct gggtgtgcag
      961 cctatggctt cgtgtgtgac gggactcgcc tcctggtgtt tggtgggatg gtggagtatg
     1021 ggaaatacag caatgacctc tacgaactcc aggcgagccg gtgggagtgg aagagactca
     1081 aagcaaagac gcccaaaaac gggccccctc cgtgtcctcg actcgggcac agcttctccc
     1141 ttgtgggcaa caaatgctac ctgtttgggg gtctggccaa tgatagcgag gacccaaaga
     1201 acaacattcc aaggtacctg aatgacttat atatcctgga attacggcca ggctctggag
     1261 tggtagcctg ggacattccc atcacttacg gggtcctacc accaccccgg gagtcacata
     1321 ctgccgtggt ctacaccgaa aaagacaata agaagtccaa gctggtgatc tacggcggga
     1381 tgagtggctg caggctgggg gacctgtgga ccctagatat tgacaccctg acgtggaata
     1441 agcccagtct cagcggggtg gcgcctcttc ctcgcagtct ccactcggca accaccatcg
     1501 gaaataaaat gtacgtgttt ggtggctggg tgcctctcgt catggatgac gtcaaagtgg
     1561 ccacacacga gaaggagtgg aagtgtacca acacgctggc ttgtctcaac ctggatacca
     1621 tggcctggga gaccatcctg atggatacac tggaggacaa catcccccgt gctcgggctg
     1681 gccactgcgc agtcgccatc aacacccgcc tgtacatttg gagtgggcgt gacggctacc
     1741 gcaaggcctg gaacaaccag gtctgctgca aggacctctg gtacctagag acagaaaagc
     1801 caccaccccc agcccgagta caactggtac gcgccaacac caactccctg gaggtgagct
     1861 ggggggcagt ggcaacagcc gacagctacc ttctccagct ccagaaatat gacattcctg
     1921 ccacggctgc tactgccacc tcccctacac ccaatccggt cccatctgtg cctgccaacc
     1981 ctcccaagag ccctgcccca gcagcagccg cacctgctgt gcagccgctg acccaagtag
     2041 gcatcacgct cctgccccag taggatcctg agaacttcag ggtgagtttg gggacccttg
     2101 attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca
     2161 gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt
     2221 gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt
     2281 ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg
     2341 tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta
     2401 tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta
     2461 gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact
     2521 acaccctggt catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga
     2581 tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct
     2641 tctctttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca
     2701 aagaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     2761 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     2821 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     2881 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     2941 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     3001 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     3061 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     3121 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     3181 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgagg
     3241 agctttttgc aaaagcctag gcctccaaaa aagcctcttc actacttctg gaatagctca
     3301 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     3361 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     3421 gttgctgact aattgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3481 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3541 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4081 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5401 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5461 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5521 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5581 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5641 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5701 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     5761 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5821 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5881 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5941 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6001 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6061 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6121 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6181 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6241 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6301 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     6361 atagggcgaa ttcgagctcg cccc
//
