LOCUS       sequence-193281-        6763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 450-1011 of HCF-1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Wilson AC, Freiman RN, Goto H, Nishimoto T, Herr W
  TITLE     VP16 targets an amino-terminal domain of HCF involved in cell cycle 
            progression.
  JOURNAL   Mol Cell Biol. 1997 Oct;17(10):6139-46.
  PUBMED    9315674
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-10"; volume: "17(10)"; pages: "6139-46"
COMMENT     Sequence Label: pCGN-HCF-1-N450-1011
FEATURES             Location/Qualifiers
     source          1..6763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             1784..1801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          2461..3033
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3165..3184)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3230..3285
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3284..3303)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3639..3774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3880..3897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4051..4639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4131..4150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4810..5601,5602..5670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5433..5452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5671..5775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5843..5861
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5899..5921)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6021..6040
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6107..6562)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6244..6265)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6456..6475
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6688..6710
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6702..6719
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6703..6719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6726..6745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6726..6744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(6763,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agatcacgtc
      721 gtgcatctgg tacgtacgga cgcgcccagg ctgcccccgc acccccgacc accaccacca
      781 tccaggtctt gccaacggtg cctggcagct ccatttctgt gcccaccgca gccaggactc
      841 aaggtgtccc tgctgttctc aaagtgaccg gtcctcaggc tacaacagga actccattgg
      901 tcaccatgcg acctgccagc caggctggga aagcccctgt caccgtgacc tcccttcccg
      961 ccggagtgcg gatggttgtg ccaacacaga gtgcccaggg aacggtgatt ggcagtagcc
     1021 cacagatgag tgggatggcc gcactggccg ctgcggccgc tgccacccag aagatccccc
     1081 cttcctcggc acccacggtg ctgagtgtcc cagcgggtac caccatcgtg aagaccatgg
     1141 ctgtgacacc tggcactacc accctcccag ccactgtgaa ggtggcctcc tcgccagtca
     1201 tggtgagcaa ccctgccact cgcatgctga agactgcagc cgcccaggtg gggacatcgg
     1261 tttcctccgc caccaacacg tctacccgcc ctatcatcac agtgcacaag tcaggcactg
     1321 tgacagtggc ccagcaagcc caggtggtga ccacagttgt gggcggggtc accaagacca
     1381 tcaccctggt gaagagcccc atctctgtcc caggaggcag tgctctgatt tccaatctgg
     1441 gcaaagtgat gtcggtggtc cagaccaaac cagttcagac ttcagcagtc acaggccagg
     1501 cgtccacggg tcctgtgact cagatcatcc agaccaaagg gcccctgcca gcgggaacaa
     1561 tcctgaagct ggtgacctca gcagatggca agcccaccac catcatcact accacgcagg
     1621 ccagtggggc ggggaccaag cccaccatcc tgggcatcag cagcgtctcc cccagtacca
     1681 ccaagcccgg cacgaccacc atcatcaaaa ccatccccat gtcggccatc atcacccagg
     1741 cgggcgccac gggtgtgacc agcagtcctg gcatcaagtc ccccatcacc atcatcacca
     1801 ccaaggtgat gacttcagga actggagcac ctgcgaaaat catcactgct gtccccaaaa
     1861 ttgccactgg ccacgggcag cagggagtga cccaggtggt gcttaagggg gccccgggac
     1921 agccaggcac catcctccgc actgtgccca tggggggtgt tcgcctggtc acacccgtca
     1981 ccgtctccgc cgtcaagcca gccgtcacca cgttggttgt gaaaggcacc acaggtgtca
     2041 cgaccctagg cacagtgaca ggcaccgtct ccaccagcct tgccggggcg gggggccaca
     2101 gcactagtgc ttccctggcc acgcccatca ccaccttggg caccattgcc accctctcaa
     2161 gccaggtgat caaccccact gccatcactg tgtcggccgc acagaccacg ctgacagcgg
     2221 caggcgggct cacaacccca accatcacca tgcagcccgt gtcccagccc acccaggtaa
     2281 ctctgatcac ggcacctagt ggggtggagg cccagcctgt gcatgacctc cctgtgtcca
     2341 ttctggcctc cccgactaca gaacagccca ccgccacagt taccatcgcc gactcaggcc
     2401 agggtgatgt gcagcctggc actgtcacct tgagatcttg aggatcctga gaacttcagg
     2461 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
     2521 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
     2581 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
     2641 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     2701 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     2761 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     2821 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     2881 cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa
     2941 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     3001 aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg
     3061 ctgtctcatc attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg
     3121 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     3181 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     3241 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     3301 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     3361 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     3421 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     3481 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     3541 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     3601 ttatggagat ccctcgagga gctttttgca aaagcctagg cctccaaaaa agcctcttca
     3661 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     3721 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     3781 agttaggggc gggactatgg ttgctgacta attgctgcat taatgaatcg gccaacgcgc
     3841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     5821 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     5881 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     5941 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     6001 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6061 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     6121 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6181 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6241 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     6301 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     6361 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     6421 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6481 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6541 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     6601 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     6661 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     6721 aattgtaata cgactcacta tagggcgaat tcgagctcgc ccc
//
