LOCUS       sequence-193399-        6008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6008)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DPAGT1 (DPAGT1A-c005)
FEATURES             Location/Qualifiers
     source          1..6008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1614..1748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1651..1670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1705..1724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1777..1942
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2126..2580
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2213..2232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2422..2443
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2606..2710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2711..2779,2780..3571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2929..2948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3742..4330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4231..4250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4484..4501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4635..4859
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4926..5459)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5629..5634,5635..5651,5652..5657))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgt gggccttctc ggaattgccc
      241 atgccgctgc tgatcaattt gatcgtctcg ctgctgggat ttgtggccac agtcaccctc
      301 atcccggcct tccggggcca cttcattgct gcgcgcctct gtggtcagga cctcaacaaa
      361 accagccgac agcagatccc agaatcccag ggagtgatca gcggtgctgt tttccttatc
      421 atcctcttct gcttcatccc tttccccttc ctgaactgct ttgtgaagga gcagtgtaag
      481 gcattccccc accatgaatt tgtggccctg ataggtgccc tccttgccat ctgctgcatg
      541 atcttcctgg gctttgcgga tgatgtactg aatctgcgct ggcgccataa gctgctgcta
      601 cctacagctg cctcactacc tctcctcatg gtctatttca ccaactttgg caacacgacc
      661 attgtggtgc ccaagccctt ccgcccgata cttggcctgc atctggactt gggaatcctg
      721 tactatgtct acatggggct gctggcagtg ttctgtacca atgccatcaa tatcctagca
      781 ggaattaacg gcctagaggc tggccagtca ctagtcattt ctgcttccat cattgtcttc
      841 aacctggtag agttggaagg tgattgtcgg gatgatcatg tcttttccct ctacttcatg
      901 ataccctttt ttttcaccac tttgggattg ctctaccaca actggtaccc atcacgggtg
      961 tttgtgggag ataccttctg ttactttgct ggcatgacct ttgccggggt gggcatcttg
     1021 ggacacttca gcaagaccat gctactattc ttcatgcccc aggtgttcaa cttcctctac
     1081 tcactgcctc agctcctgca tatcatcccc tgccctcgcc accgcatacc cagactcaat
     1141 atcaagacag gcaaactgga gatgagctat tccaagttca agaccaagag cctctctttc
     1201 ttgggcacct ttattttaaa ggtggcagag agcctccagc tggtgacagt acaccagagt
     1261 gagactgaag atggtgaatt cactgaatgt aacaacatga ccctcatcaa cttgctactt
     1321 aaagtccttg ggcccataca tgagagaaac ctcacattgc tcctgctgct gctgcagatc
     1381 ctgggcagtg ccatcacctt ctccattcga tatcagctcg ttcgactctt ctatgatgtc
     1441 tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt gtcgagaagt
     1501 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1561 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1741 tctggatctg atcactgata tcgcctagga gatccgaacc agataagtga aatctagttc
     1801 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     1861 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     1921 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
     1981 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
     2041 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
     2101 atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2161 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2221 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2281 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2341 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2401 ttggagtcca cgttcttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2461 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2521 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     2581 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2641 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2941 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3241 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3421 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4441 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4501 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4561 ccgcatagac cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc
     4621 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
     4681 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
     4741 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
     4801 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg
     4861 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg
     4921 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
     4981 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
     5041 taagcaccaa gcgcgttggc ctcatgcttg agcagattga tgagcgcggt ggcaatgccc
     5101 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc
     5161 tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca
     5221 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga
     5281 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc
     5341 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca
     5401 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     5461 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     5521 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     5581 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     5641 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     5701 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     5761 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     5821 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     5881 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     5941 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat actccggaat
     6001 attaatag
//
