LOCUS       sequence-193509-        9678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Jones JA, Vernacchio VR, Collins SM, Shirke AN, Xiu Y, Englaender 
            JA, Cress BF, McCutcheon CC, Linhardt RJ, Gross RA, Koffas MAG
  TITLE     Complete Biosynthesis of Anthocyanins Using E. coli Polycultures.
  JOURNAL   MBio. 2017 Jun 6;8(3). pii: e00621-17. doi: 10.1128/mBio.00621-17.
  PUBMED    28588129
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2017-06-6"; volume: "8(3). pii: e00621-17. doi"; pages: " 
            10.1128/mBio.00621-17"
COMMENT     Sequence Label: pETM6-RgTALsyn-HpaBC
FEATURES             Location/Qualifiers
     source          1..9678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     970..989
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1009..1086)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1292..1311
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        1402..1420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1421..1445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1460..1482
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      3621..3668
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3702..3720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3721..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3760..3782
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      5401..5448
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5482..5500
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5501..5525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5540..5562
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      6131..6178
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6222..6677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6519..6540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(6792..7583,7584..7652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7415..7434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7653..7745)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7826..8414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8315..8334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8568..8585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8600..8739
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8665..8684)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8825..8847
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8841..9032)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9604..9678,1..1008))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
       61 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
      121 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
      181 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
      241 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
      301 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
      361 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
      421 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
      481 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
      541 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
      601 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
      661 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
      721 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
      781 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
      841 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
      901 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
      961 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     1021 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     1081 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     1141 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     1201 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     1261 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     1321 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagcct aggatcgaga
     1381 tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     1441 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tggcgcctcg
     1501 cccgacttcg caaagccagg cccgcacttg cccgacgacg caggttaccc aagttgatat
     1561 cgttgagaaa atgttggcgg ctcctactga tagcacgctg gagctggacg gttatagcct
     1621 gaatctgggt gatgtcgtga gcgctgcgcg taagggtcgt cctgtccgtg tcaaagatag
     1681 cgatgaaatc cgcagcaaaa tcgacaagag cgttgaattc ctgcgcagcc aactgagcat
     1741 gtcggtttac ggtgtgacga ccggctttgg cggctccgcg gacacgcgca cggaggacgc
     1801 aattagcctg caaaaggcgt tgctggaaca ccagctgtgt ggtgtgttgc cgagcagctt
     1861 cgacagcttt cgcttgggtc gtggtctgga gaatagcctg ccgttggaag tcgttcgcgg
     1921 tgcaatgacc attcgtgtga attcgctgac ccgtggccat agcgctgttc gtctggttgt
     1981 tctggaagca ctgacgaact ttctgaacca cggtattacc ccgattgttc cgctgcgcgg
     2041 tacgatctcc gcgagcggcg atctgtctcc actgtcgtac attgcagcgg cgattagcgg
     2101 tcacccggat agcaaagttc acgtggtcca tgaaggcaaa gagaagatcc tgtacgcgcg
     2161 cgaagcgatg gcgctgttta acctggagcc ggtggttttg ggtccgaagg agggcctggg
     2221 tctggtgaat ggtacggcag tctccgcgag catggcaacg ctggcactgc acgacgcgca
     2281 catgttgagc ctgttgagcc aatcgctgac cgcgatgacc gtggaggcga tggtcggtca
     2341 cgcgggcagc ttccatccat tcctgcacga tgttacgcgt ccgcacccga cgcaaatcga
     2401 ggtcgcgggt aacattcgca aactgctgga gggctcgcgc ttcgcggtcc accacgagga
     2461 agaggttaag gtcaaggatg atgaaggcat tttgcgtcag gatcgttatc cgttgcgcac
     2521 gagcccgcaa tggttgggtc cgctggtgtc cgacctgatt cacgctcatg ccgtcttgac
     2581 gatcgaagcg ggtcaaagca ccaccgataa cccactgatc gatgttgaga ataagaccag
     2641 ccatcacggt ggcaactttc aagcggcagc ggttgccaac acgatggaaa agacccgtct
     2701 gggcttggcc caaatcggta aactgaattt cacccagctg acggagatgc tgaacgcggg
     2761 catgaatcgt ggcttgccga gctgcctggc ggctgaagac ccatccctga gctatcattg
     2821 caaaggtctg gacattgcgg cggctgcata tacgagcgaa ctgggccacc tggctaaccc
     2881 ggtcaccacc cacgtccaac cggctgaaat ggcaaaccag gcggtgaata gcttggcgtt
     2941 gattagcgca cgtcgtacca cggaatctaa cgacgttctg tccctgctgc tggcaacgca
     3001 cctgtactgc gtgctgcagg cgatcgacct gcgtgcgatt gagttcgagt tcaagaaaca
     3061 gtttggtcct gccattgtta gcctgatcga ccaacacttt ggtagcgcga tgacgggtag
     3121 caatctgcgt gatgagctgg ttgaaaaggt caataagact ctggccaagc gtttggagca
     3181 aaccaatagc tacgatctgg ttccgcgctg gcacgacgct tttagcttcg ctgcaggcac
     3241 tgttgtcgag gttctgtcca gcacgagcct gagcttggcg gccgtgaacg catggaaggt
     3301 tgcggcagcc gagagcgcga tctccttgac gcgccaggtc cgtgaaacgt tttggtccgc
     3361 tgcaagcacc tccagcccgg cgttgtctta cttgagcccg cgcacgcaga tcctgtacgc
     3421 atttgtgcgt gaggaactgg gtgtcaaagc ccgccgtggt gacgtcttct tgggtaaaca
     3481 agaagttacc atcggcagca acgttagcaa gatttacgaa gccatcaaga gcggccgtat
     3541 caacaatgtt ctgctgaaga tgctggcata aactagtcgc agcttaatta acctaaactg
     3601 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     3661 gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg
     3721 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
     3781 gatatacata tgaaaccaga agatttccgc gccagtaccc aacgtccttt caccggggaa
     3841 gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa
     3901 gacgtcacca ctcatccggc atttcgtaat gcggcagcgt ctgttgccca gctgtacgac
     3961 gcactgcaca aaccggagat gcaggactct ctgtgttgga acaccgacac cggcagcggc
     4021 ggctataccc ataaattctt ccgcgtggcg aaaagtgccg acgacctgcg ccagcaacgc
     4081 gacgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac
     4141 aaagccgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag
     4201 aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt
     4261 gttaacccac cgatcgatcg tcatttgccg accgataaag tgaaagacgt ttacatcaag
     4321 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
     4381 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
     4441 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa attaatctcc
     4501 cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta cccgctctcc
     4561 agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat tccatgggaa
     4621 aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga aggcggtttt
     4681 gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga cttcattacg
     4741 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtgcaggcc
     4801 gatctcggtg aagtggtagc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
     4861 tcagaagcaa cgccgtgggt caacggggct tatttaccgg atcatgccgc actgcaaacc
     4921 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt
     4981 accagtggcc tgatctatct cccttccagt gcccgtgacc tgaataatcc gcagatcgac
     5041 cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca gcgcatcaag
     5101 atcctcaaac tgatgtggga tgctattggc agcgaatttg gtggtcgtca cgaactgtat
     5161 gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg ccaggcacaa
     5221 aactccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
     5281 caggacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat gctggataag
     5341 ctgctgaaat aactagtcgc agcttaatta acctaaactg ctgccaccgc tgagcaataa
     5401 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct aggatcgaga
     5461 tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     5521 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaattaga
     5581 tgaacaacgc ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat
     5641 caccaccgag ggcgacgccg gacaatgcgg gattacggca acggccgtct gctcggtcac
     5701 ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt
     5761 tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg
     5821 ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca
     5881 aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggtgagat
     5941 ccgcgatgtg caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat
     6001 cctcagtgca gaaggtcatg gacttatcta ctttaaacgc cgtttccatc cggtgatgct
     6061 ggaaatggaa gctgcgattt aactagtcgc agcttaatta acctaaactg ctgccaccgc
     6121 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     6181 agcgaaagga ggagtcgact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
     6241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6661 attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag gatcttcacc
     6721 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6781 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6841 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6901 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6961 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7021 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7081 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7141 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7201 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7261 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7321 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7381 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7441 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7501 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7561 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7621 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc atgattgaag
     7681 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7741 acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7801 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7861 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7921 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7981 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8041 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8101 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8161 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8221 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8281 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8341 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8401 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8461 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8521 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8581 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacacatctg tgcggtattt
     8641 cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8701 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     8761 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8821 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     8881 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     8941 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     9001 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     9061 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     9121 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     9181 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     9241 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     9301 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatattgtt
     9361 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     9421 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     9481 acgatcatgc tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     9541 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     9601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9661 caacgcgcgg ggagaggc
//
