LOCUS       sequence-193607-       11107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of human SPDEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF
  TITLE     SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the 
            Transcriptional Targets of beta-catenin.
  JOURNAL   Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 
            10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5.
  PUBMED    28390865
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2017.03.048"; journalName: "Gastroenterology"; 
            date: "2017-07"; volume: "153"; issue: "1"; pages: "205-218.e8"
COMMENT     Sequence Label: pINDUCER20-Flag-hSPDEF WT
FEATURES             Location/Qualifiers
     source          1..11107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        178..216
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     213..237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    346..370
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             376..441
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1469..1493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1546..1572
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1658..2869
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2706..2725
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2928..3545,3546..3551,3552..3587,3588..3590,3591..3626,
                     3627..3629,3630..3665,3666..3674)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3819..4281
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3875..3892)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4102..4121
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4297..5091
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4297..5091
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4351..4370)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4961..4980
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5268..5856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5321..5341)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5739..5750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5931..6164
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6283..6301)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6369..6473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6474..6542,6543..7334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6692..6711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7505..8093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7994..8013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8247..8264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8504..8525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8540..8545,8546..8563,8564..8570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8578..8594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8583..8605
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8602..8618
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8602..8618
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8668..8687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8879..9512
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9559..9684
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10181..10414
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10599..10643
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10936..11053
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
      181 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      241 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      301 cgcggccccg aactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
      361 aaagcaggct tcatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat
      421 tacaaggatg acgatgacaa gcttgcggcc gcgaattcca tgggcagcgc cagcccgggt
      481 ctgagcagcg tatcccccag ccacctcctg ctgccccccg acacggtgtc gcggacaggc
      541 ttggagaagg cggcagcggg ggcagtgggt ctcgagagac gggactggag tcccagtcca
      601 cccgccacgc ccgagcaggg cctgtccgcc ttctacctct cctactttga catgctgtac
      661 cctgaggaca gcagctgggc agccaaggcc cctggggcca gcagtcggga ggagccacct
      721 gaggagcctg agcagtgccc ggtcattgac agccaagccc cagcgggcag cctggacttg
      781 gtgcccggcg ggctgacctt ggaggagcac tcgctggagc aggtgcagtc catggtggtg
      841 ggcgaagtgc tcaaggacat cgagacggcc tgcaagctgc tcaacatcac cgcagatccc
      901 atggactgga gccccagcaa tgtgcagaag tggctcctgt ggacagagca ccaataccgg
      961 ctgcccccca tgggcaaggc cttccaggag ctggcgggta aggagctgtg cgccatgtcg
     1021 gaggagcagt tccgccagcg ctcgcccctg ggtggggatg tgctgcacgc ccacctggac
     1081 atctggaagt cagcggcctg gatgaaagag cggacttcac ctggggcgat tcactactgt
     1141 gcctcgacca gtgaggagag ctggaccgac agcgaggtgg actcatcatg ctccgggcag
     1201 cccatccacc tgtggcagtt cctcaaggag ttgctactca agccccacag ctatggccgc
     1261 ttcattaggt ggctcaacaa ggagaagggc atcttcaaaa ttgaggactc agcccaggtg
     1321 gcccggctgt ggggcatccg caagaaccgt cccgccatga actacgacaa gctgagccgc
     1381 tccatccgcc agtattacaa gaagggcatc atccggaagc cagacatctc ccagcgcctc
     1441 gtctaccagt tcgtgcaccc catctgagac ccagctttct tgtacaaagt ggttgatatc
     1501 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaataccc atacgacgtc
     1561 ccagactacg cttagtaatg attaattaaa ctagaaattc taccgggtag gggaggcgct
     1621 tttcccaagg cagtctggag taacccaccc aagatctggc ctccgcgccg ggttttggcg
     1681 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     1741 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     1801 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     1861 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     1921 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     1981 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     2041 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     2101 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     2161 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     2221 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     2281 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     2341 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     2401 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     2461 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     2521 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     2581 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     2641 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     2701 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     2761 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     2821 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttggta
     2881 ccgagctcgg atctccaccc cgtaccggtc ctgcagtcga attcaccatg tctagactgg
     2941 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     3001 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     3061 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     3121 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     3181 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     3241 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     3301 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     3361 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     3421 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     3481 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     3541 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     3601 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     3661 tgctccccgg gtaactaagt aaggatccgc ggccgcacta gaggaattcc gcccctctcc
     3721 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3781 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3841 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3901 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3961 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4021 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     4081 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     4141 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     4201 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4261 ctttgaaaaa cacgatgata agcttaccgg tccaccatga ttgaacaaga tggattgcac
     4321 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4381 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4441 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     4501 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4561 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4621 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4681 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4741 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4801 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4861 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4921 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4981 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5041 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg atgtacaagt
     5101 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5161 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5221 tgtttagtcc ctcccaattc gatatcaagc ttatcgcgat agatcctaat caacctctgg
     5281 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5341 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5401 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5461 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5521 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5581 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5641 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5701 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5761 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5821 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     5881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5941 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6181 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
     6241 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     6301 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6361 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6421 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6481 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6541 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6601 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6661 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     6721 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6781 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6841 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6901 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6961 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7021 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7081 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7141 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7201 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7261 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7321 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7381 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7621 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8101 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8161 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8221 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8281 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
     8341 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8401 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     8461 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8521 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8581 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
     8641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8701 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     8761 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     8821 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     8881 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     8941 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     9001 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     9061 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     9121 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     9181 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     9241 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     9301 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     9361 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9421 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9481 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9541 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9601 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9661 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9721 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9781 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9841 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9901 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9961 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    10021 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    10081 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    10141 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    10201 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    10261 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    10321 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    10381 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    10441 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    10501 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    10561 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    10621 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    10681 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    10741 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
    10801 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
    10861 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
    10921 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    10981 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    11041 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctaggatcct
    11101 ttaccac
//
