LOCUS       sequence-193634-        1438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Pim1 kinase domain in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Lee SJ, Han BG, Cho JW, Choi JS, Lee J, Song HJ, Koh JS, Lee BI
  TITLE     Crystal structure of pim1 kinase in complex with a 
            pyrido[4,3-d]pyrimidine derivative suggests a unique binding mode.
  JOURNAL   PLoS One. 2013 Jul 31;8(7):e70358. doi: 
            10.1371/journal.pone.0070358. Print 2013.
  PUBMED    23936194
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-31"; volume: "8(7):e70358. doi"; pages: " 
            10.1371/journal.pone.0070358. Print 2013"
COMMENT     Sequence Label: pHis-hPim1
FEATURES             Location/Qualifiers
     source          1..1438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             929..952
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(1015..1033)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1029..1076
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gagtaaacgg ttcattccct ctagatttaa ttaactttaa gaaggagata tacatatgaa
       61 ggagaaggag cccctggagt cgcagtacca ggtgggcccg ctactgggca gcggcggctt
      121 cggctcggtc tactcaggca tccgcgtctc cgacaacttg ccggtggcca tcaaacacgt
      181 ggagaaggac cggatttccg actggggaga gctgcctaat ggcactcgag tgcccatgga
      241 agtggtcctg ctgaagaagg tgagctcggg tttctccggc gtcattaggc tcctggactg
      301 gttcgagagg cccgacagtt tcgtcctgat cctggagagg cccgagccgg tgcaagatct
      361 cttcgacttc atcacggaaa ggggagccct gcaagaggag ctggcccgca gcttcttctg
      421 gcaggtgctg gaggccgtgc ggcactgcca caactgcggg gtgctccacc gcgacatcaa
      481 ggacgaaaac atccttatcg acctcaatcg cggcgagctc aagctcatcg acttcgggtc
      541 gggggcgctg ctcaaggaca ccgtctacac ggacttcgat gggacccgag tgtatagccc
      601 tccagagtgg atccgctacc atcgctacca tggcaggtcg gcggcagtct ggtccctggg
      661 gatcctgctg tatgatatgg tgtgtggaga tattcctttc gagcatgacg aagagatcat
      721 caggggccag gttttcttca ggcagagggt ctcttcagaa tgtcagcatc tcattagatg
      781 gtgcttggcc ctgagaccat cagataggcc aaccttcgaa gaaatccaga accatccatg
      841 gatgcaagat gttctcctgc cccaggaaac tgctgagatc cacctccaca gcctgtcgcc
      901 ggggcccagc aaagcggccg cactcgagca ccaccaccac caccaccacc actaattgat
      961 taatacctag gctgctaaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1021 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     1081 aaggaggact atatccggat tgcgaatggg acccccctgt accgcgcatt aacgcggcgg
     1141 gtgtgtggtt acgcgagcgt gaccgttacc ttgcaagggc ctaaggcccg ttctttcctt
     1201 tcttccttct tttccgccag ttcccgggtt tcccggcaag tctaaatcgg gggtcccttt
     1261 agggtccgat ttaggcttta cggccctcgc cccaaaaact ggttagggga aggtccctat
     1321 ggggccaccc ccctaaaacg gttttcgcct ttgagtggaa ccacgtcttt tagagggact
     1381 ttgtcaaagg gaaacccaac ccttccgggg tcttttttat aaagagattc ccaactcc
//
