LOCUS       sequence-193670-       10043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA expression construction in a 3rd generation lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-sgRNA-Lib
FEATURES             Location/Qualifiers
     source          1..10043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1865..1909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2404..2652
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2404..2424
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2575..2594
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     2676..2695
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2676..2694
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2724..2848
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3277..3582)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3379..3398)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3902..4585)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     4500..4519
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4586..4688)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4866..4990)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_RNA        5023..5098
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     5108..5124
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5166..5199)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5315..5618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5619..5822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5861..5870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5867..5869,5870..5872,5873..6586)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5867..5869,5870..5872,5873..6583)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5912..5933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6173..6192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6520..6541
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6606..6639)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6695..7283
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6748..6768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7166..7177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7286..7302)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7812..7992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8054..8642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8134..8153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8813..9604,9605..9673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9436..9455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9674..9778)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9853..9872)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gaggtaccaa ggtctggcag
     2401 gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     2461 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     2521 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     2581 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2641 ggacgaaaca ccgagagacg ctggcttatc gaaattaata cgactcacta tagggagacc
     2701 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     2761 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2821 gtaaaacaca acatatccag tcactatgaa tcaactactt agatggtatt agtgacctgt
     2881 agtcgaccga cagccttcca aatgttcttc gggtgatgct gccaacttag tcgaccgaca
     2941 gccttccaaa tgttcttctc aaacggaatc gtcgtatcca gcctactcgc tattgtcctc
     3001 aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc ttttttgtgt
     3061 gacaaaataa aaacatctac ctattcatat acgctagtgt catagtcctg aaaatcatct
     3121 gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc ggcttcatct
     3181 ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc tactgtatcg
     3241 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
     3301 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     3361 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     3421 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
     3481 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     3541 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
     3601 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
     3661 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     3721 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     3781 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata
     3841 tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct
     3901 tttagtaagc cggatccacg cggcgtttac gccccccctg ccactcatcg cagtactgtt
     3961 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     4021 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     4081 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4141 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     4201 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4261 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4321 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     4381 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4441 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4501 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     4561 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     4621 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     4681 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     4741 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     4801 gtgcgtcggg tgatgctgcc aacttagtcg actacaggtc actaatacca tctaagtagt
     4861 tgattcatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     4921 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     4981 acaaagtggt tgatctagag ggcccgcggt tcgaacgtct ctgttttaga gctagaaata
     5041 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     5101 tttttctcga ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg
     5161 acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag
     5221 cggccgcgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     5281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     5341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     5401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     5461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     5521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     5581 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5641 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     5701 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     5761 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     5821 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
     5881 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5941 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     6001 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     6061 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     6121 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     6181 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     6241 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     6301 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     6361 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     6421 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     6481 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     6541 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaggaat tcgtcgaggg
     6601 acctaataac ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt
     6661 ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     6721 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6781 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6841 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6901 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6961 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7021 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7081 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7141 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7201 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7261 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     7321 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7381 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7441 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7501 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7561 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7621 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7681 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7741 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7801 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7861 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7921 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7981 aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8041 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8101 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8161 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8221 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8281 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8341 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8401 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8461 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8521 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8581 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8641 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8701 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8761 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8821 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8881 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8941 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9001 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9061 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9121 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9181 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9241 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9301 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9361 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9421 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9481 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9541 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9601 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9661 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9721 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9781 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9841 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9901 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9961 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    10021 tgtacgggcc agatatacgc gtt
//
