LOCUS       sequence-19405-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-2T-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(28..72,73..75,76..603)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="QAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     CDS             28..603
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="QAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(662..684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcggatctgg ttccgcgtgg atccccgcag gccatcaagt gtgtggtggt gggagacgga
       61 gctgtaggta aaaattgcct actgatcagt tacacaacca atgcatttcc tggagaatat
      121 atccctactg tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat
      181 ctgggcttat gggatacagc tggacaagaa gattatgaca gattacgccc cctatcctat
      241 ccgcaaacag atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat
      301 gtccgtgcaa agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta
      361 gtgggaacta aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag
      421 aagctgactc ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgccgta
      481 aaatacctgg agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg
      541 atccgagcag tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg
      601 taagaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa
      661 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      781 cccagtcacg tagcgatag
//
