LOCUS       sequence-19406-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-2T-Rac1-Q61L
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(31..207,208..210,211..606)
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(665..687)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 caatcggatc tggttccgcg tggatccccg caggccatca agtgtgtggt ggtgggagac
       61 ggagctgtag gtaaaacttg cctactgatc agttacacaa ccaatgcatt tcctggagaa
      121 tatatcccta ctgtctttga caattattct gccaatgtta tggtagatgg aaaaccggtg
      181 aatctgggct tatgggatac agctggacta gaagattatg acagattacg ccccctatcc
      241 tatccgcaaa cagatgtgtt cttaatttgc ttttcccttg tgagtcctgc atcatttgaa
      301 aatgtccgtg caaagtggta tcctgaggtg cggcaccact gtcccaacac tcccatcatc
      361 ctagtgggaa ctaaacttga tcttagggat gataaagaca cgatcgagaa actgaaggag
      421 aagaagctga ctcccatcac ctatccgcag ggtctagcca tggctaagga gattggtgcc
      481 gtaaaatacc tggagtgctc ggcgctcaca cagcgaggcc tcaagacagt gtttgacgaa
      541 gcgatccgag cagtcctctg cccgcctccc gtgaagaaga ggaagagaaa atgcctgctg
      601 ttgtaagaat tcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg
      661 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      721 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcg
//
