LOCUS       sequence-19408-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..79
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(92..139,140..142,143..670)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     CDS             92..670
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     regulatory      690..699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcccaggtcc aactgcacct cggttctatc gattgaattg gccaccatgg agcagaagct
       61 gatctccgag gaggacctgg gatccggtac catgcaggcc atcaagtgtg tggtggtggg
      121 agacggagct gtaggtaaaa attgcctact gatcagttac acaaccaatg catttcctgg
      181 agaatatatc cctactgtct ttgacaatta ttctgccaat gttatggtag atggaaaacc
      241 ggtgaatctg ggcttatggg atacagctgg acaagaagat tatgacagat tacgccccct
      301 atcctatccg caaacagatg tgttcttaat ttgcttttcc cttgtgagtc ctgcatcatt
      361 tgaaaatgtc cgtgcaaagt ggtatcctga ggtgcggcac cactgtccca acactcccat
      421 catcctagtg ggaactaaac ttgatcttag ggatgataaa gacacgatcg agaaactgaa
      481 ggagaagaag ctgactccca tcacctatcc gcagggtcta gccatggcta aggagattgg
      541 tgccgtaaaa tacctggagt gctcggcgct cacacagcga ggcctcaaga cagtgtttga
      601 cgaagcgatc cgagcagtcc tctgcccgcc tcccgtgaag aagaggaaga gaaaatgcct
      661 gctgttgtaa gaattcctgc agaagcttgg ccgccatggc ccaacttgtt tattgcagct
      721 tataat
//
