LOCUS       sequence-194096-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro expression of sfGFP from the T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante TC, 
            Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq
            D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, 
            Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, 
            Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen
            B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, 
            Donghia N, Pardee K, Collins JJ, Jewett MC
  TITLE     BioBits Bright: A fluorescent synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. 
            eCollection 2018 Aug.
  PUBMED    30083609
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5107. doi"; pages: " 
            10.1126/sciadv.aat5107. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1-sfGFP
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             649..696
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             697..720
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgagcaaag gtgaagaact gtttaccggc gttgtgccga ttctggtgga actggatggc
       61 gatgtgaacg gtcacaaatt cagcgtgcgt ggtgaaggtg aaggcgatgc cacgattggc
      121 aaactgacgc tgaaatttat ctgcaccacc ggcaaactgc cggtgccgtg gccgacgctg
      181 gtgaccaccc tgacctatgg cgttcagtgt tttagtcgct atccggatca catgaaacgt
      241 cacgatttct ttaaatctgc aatgccggaa ggctatgtgc aggaacgtac gattagcttt
      301 aaagatgatg gcaaatataa aacgcgcgcc gttgtgaaat ttgaaggcga taccctggtg
      361 aaccgcattg aactgaaagg cacggatttt aaagaagatg gcaatatcct gggccataaa
      421 ctggaataca actttaatag ccataatgtt tatattacgg cggataaaca gaaaaatggc
      481 atcaaagcga attttaccgt tcgccataac gttgaagatg gcagtgtgca gctggcagat
      541 cattatcagc agaatacccc gattggtgat ggtccggtgc tgctgccgga taatcattat
      601 ctgagcacgc agaccgttct gtctaaagat ccgaacgaaa aaggcacgcg ggaccacatg
      661 gttctgcacg aatatgtgaa tgcggcaggt attacgtgga gccatccgca gttcgaaaaa
      721 taa
//
