LOCUS       sequence-194142-        3755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea PAM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: PAM
FEATURES             Location/Qualifiers
     source          1..3755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(634..652)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(635..652)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(656..674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      688..735
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(747..764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(918..1506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(998..1017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1677..2468,2469..2537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2300..2319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2555..3349)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2666..2685)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3276..3295
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt agacggccat
      121 ttaccaacag gtagtcattg aaccagatca catcgggtgg atttcttttc atttagaaaa
      181 tgcaaatcaa attgaaaatg gtattaatat tggttttcat gtcgttccag atgaagattt
      241 gcatgcaagt gctgcaaatg gaatggcaaa tgataaaatt agagtaagag cagtaaaggt
      301 tctggatgct tcggaatcag tttctactac ttactataca gaggatgatc aattgaagtt
      361 gagatatgat ctgttgaaag aagaaacttt gaaatctttc attgaaatga caaatcatat
      421 cttttacaat tgttttacaa cctcatatca gaacagaata gaaaagaaag agcttcaaga
      481 taatcatgac atcgaaagta aaagtcttca tgattttctt gttaacattt tgtttgacac
      541 tggattcaaa tcggaagtca tttcgtcaag attgagtagc ttgcaacgaa gggtttaatc
      601 tcatatcatt actcaaatta atgtaaggtc acttctatag tgtcacctaa atctccctat
      661 agtgagtcgt attaggatcc ggcgcgccta gcataacccc ttggggcctc taaacgggtc
      721 ttgaggggtt ttttgacgcg tgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1741 gcctggctcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2521 aaatgttgaa tactcatact cttccttttt caattcagaa gaactcgtca agaaggcgat
     2581 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     2641 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     2701 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     2761 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     2821 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     2881 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     2941 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     3001 ccatgatgga tactttctcg gcaggagcaa ggtgggatga caggagatcc tgccccggca
     3061 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     3121 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     3181 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     3241 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     3301 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     3361 ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcatt ggcggcaaga
     3421 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     3481 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac
     3541 tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     3601 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc
     3661 tttagcagcc cttgcgccct gaattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3721 ctcatttttt aaccaatagg ccgaaatcga cgcgt
//
