LOCUS       sequence-194244-        3696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea TRF2-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: TRF2-2
FEATURES             Location/Qualifiers
     source          1..3696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(575..593)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(597..615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      629..676
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1083..1877
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1137..1156)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1747..1766
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1895..1963,1964..2755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2113..2132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2926..3514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3415..3434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3668..3685
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct tttgtgatgc
      121 ctacacctca gttacaaaat attgtttgca ctgtaaattt gggatgtaaa ttagatttga
      181 aatcaattgc tcttcatgcc agaaacgctg aatacaatcc aaagcgtttt gcagccgtta
      241 ttatgagaat aagaacacct cgaactaccg ctcttatatt ttcatcgggt aaaatggttt
      301 gtactggcgc aaaaagtgaa gatgaagcta aattagcagc gagaaagttt gccagaatta
      361 ttcaaaaatt agattttgat gcaaaattta tgaattttaa aattcaaaac atggttggtt
      421 cttgtgacgt caaatttcca attcgtttgg agggtatttg tctttcacac acccagtttg
      481 ctacatatga accagaattg tttcctggat taatttatcg aatgggtaaa ccaaaaattg
      541 ttcttttgat tttcgtttcg ggaaaaaagg tctcccttta gtgagggtta attctcccta
      601 tagtgagtcg tattaggatc cggcgcgcct agcataaccc cttggggcct ctaaacgggt
      661 cttgaggggt tttttgacgc gtcgatttcg gcctattggt taaaaaatga gctgatttaa
      721 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
      781 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
      841 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
      901 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
      961 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     1021 caatgatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     1081 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     1141 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     1201 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     1261 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     1321 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     1381 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     1441 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     1501 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     1561 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     1621 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     1681 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     1741 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     1801 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     1861 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1921 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1981 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2041 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2101 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2161 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2221 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2281 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2341 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2401 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2461 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2521 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2581 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2641 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagccagg caactatgga
     2701 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2761 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2821 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3061 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3601 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3661 cgagcgcagc gagtcagtga gcgaggaagc acgcgt
//
