LOCUS       sequence-194304-       14258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14258)
  AUTHORS   Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen
            PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, 
            Granter SR, Turley SJ, Fisher DE, Novina CD
  TITLE     Intronic miR-211 assumes the tumor suppressive function of its host 
            gene in melanoma.
  JOURNAL   Mol Cell. 2010 Dec 10. 40(5):841-9.
  PUBMED    21109473
REFERENCE   2  (bases 1 to 14258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Dec 10. 40(5):841-9."
COMMENT     Sequence Label: PLKO-mcherry-luc-puro-TRPM1 cDNA
FEATURES             Location/Qualifiers
     source          1..14258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..87
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    204..225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(240..245,246..263,264..270)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    278..294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     283..305
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     302..318
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     302..318
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     337..357
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        339..357
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        385..611
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             612..792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    839..964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1457..1690
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1875..1919
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2094..2604
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     misc_feature    7291..7408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7457..7967
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     regulatory      7976..7985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9641..9700
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(9852..9870)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             10430..10483
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             11161..11394
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11466..11587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11503..11522)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11557..11576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11627..11762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11782..11801)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11783..11801)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11811..11828)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11811..11827)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11820..11842)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11969..12424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12056..12075)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12266..12287
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12450..12554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12555..12623,12624..13415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12773..12792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13586..14174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14075..14094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
       61 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      121 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      181 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      241 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      301 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      361 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
      421 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
      481 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
      541 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
      601 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      781 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      841 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      901 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      961 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     1021 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     1081 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     1141 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     1201 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     1261 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     1321 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1381 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1441 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1501 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1561 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1621 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1681 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1741 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1801 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1861 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1921 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1981 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     2041 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccggggttg
     2101 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     2161 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     2221 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     2281 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     2341 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     2401 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     2461 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     2521 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     2581 cgaatcaccg acctctctcc ccagggaccg gtatgtatat ccgtgtatcc tataacacca
     2641 agccagactc actgctccat ctcatggtga aagattggca gctggaactc cccaagctct
     2701 taatatctgt gcatggaggc ctccagaact ttgagatgca gcccaagctg aaacaagtct
     2761 ttgggaaagg cctgatcaag gctgctatga ccaccggggc ctggatcttc accgggggtg
     2821 tcagcacagg tgttatcagc cacgtagggg atgccttgaa agaccactcc tccaagtcca
     2881 gaggccgggt ttgtgctata ggaattgctc catggggcat cgtggagaat aaggaagacc
     2941 tggttggaaa ggatgtaaca agagtgtacc agaccatgtc caaccctcta agtaagctct
     3001 ctgtgctcaa caactcccac acccacttca tcctggctga caatggcacc ctgggcaagt
     3061 atggcgccga ggtgaagctg cgaaggctgc tggaaaagca catctccctg cagaagatca
     3121 acacaagact ggggcagggc gtgcccctcg tgggtctcgt ggtggagggg ggccctaacg
     3181 tggtgtccat cgtcttggaa tacctgcaag aagagcctcc catccctgtg gtgatttgtg
     3241 atggcagcgg acgtgcctcg gacatcctgt cctttgcgca caagtactgt gaagaaggcg
     3301 gaataataaa tgagtccctc agggagcagc ttctagttac cattcagaaa acatttaatt
     3361 ataataaggc acaatcacat cagctgtttg caattataat ggagtgcatg aagaagaaag
     3421 aactcgtcac tgtgttcaga atgggttctg agggccagca ggacatcgag atggcaattt
     3481 taactgccct gctgaaagga acaaacgtat ctgctccaga tcagctgagc ttggcactgg
     3541 cttggaaccg cgtggacata gcacgaagcc agatctttgt ctttgggccc cactggccgc
     3601 ccctgggaag cctggcaccc ccgacggaca gcaaagccac agagaaggag aagaagccac
     3661 ccatggccac caccaaggga ggaagaggaa aagggaaagg caagaagaaa gggaaagtga
     3721 aagaggaagt ggaggaagaa actgaccccc ggaagataga gctgctgaac tgggtgaatg
     3781 ctttggagca agcgatgcta gatgctttag tcttagatcg tgtcgacttt gtgaagctcc
     3841 tgattgaaaa cggagtgaac atgcaacact ttctgaccat tccgaggctg gaggagcttt
     3901 ataacacaag actgggtcca ccaaacacac ttcatctgct ggtgagggat gtgaaaaaga
     3961 gcaaccttcc gcctgattac cacatcagcc tcatagacat cgggctcgtg ctggagtacc
     4021 tcatgggagg agcctaccgc tgcaactaca ctcggaaaaa ctttcggacc ctttacaaca
     4081 acttgtttgg accaaagagg cctaaagctc ttaaacttct gggaatggaa gatgatgagc
     4141 ctccagctaa agggaagaaa aagaaaaaga agaaaaagga ggaagagatc gacattgatg
     4201 tggacgaccc tgccgtgagt cggttccagt atcccttcca cgagctgatg gtgtgggcag
     4261 tgctgatgaa acgccagaaa atggcagtgt tcctctggca gcgaggggaa gagagcatgg
     4321 ccaaggccct ggtggcctgc aagctctaca aggccatggc ccacgagtcc tccgagagtg
     4381 atctggtgga tgacatctcc caggacttgg ataacaattc caaagacttc ggccagcttg
     4441 ctttggagtt attagaccag tcctataagc atgacgagca gatcgctatg aaactcctga
     4501 cctacgagct gaaaaactgg agcaactcga cctgcctcaa actggccgtg gcagccaaac
     4561 accgggactt cattgctcac acctgcagcc agatgctgct gaccgatatg tggatgggaa
     4621 gactgcggat gcggaagaac cccggcctga aggttatcat ggggattctt ctacccccca
     4681 ccatcttgtt tttggaattt cgcacatatg atgatttctc gtatcaaaca tccaaggaaa
     4741 acgaggatgg caaagaaaaa gaagaggaaa atacggatgc aaatgcagat gctggctcaa
     4801 gaaaggggga tgaggagaac gagcacaaaa aacagagaag tattcccatc ggaacaaaga
     4861 tctgtgagtt ctataacgcg cccattggaa caaagatctg tgagttctat aacgcgccca
     4921 ttgtcaagtt ctggttttac acaatatcat acttgggcta cctgctgctg tttaactacg
     4981 tcatcctggt gcggatggat ggctggccgt ccctccagga gtggatcgtc atctcctaca
     5041 tcgtgagcct ggcgttagag aagatacgag agatcctcat gtcagaacca ggcaaactca
     5101 gccagaaaat caaagtttgg cttcaggagt actggaacat cacagatctc gtggccattt
     5161 ccacattcat gattggagca attcttcgcc tacagaacca gccctacatg ggctatggcc
     5221 gggtgatcta ctgtgtggat atcatcttct ggtacatccg tgtcctggac atctttggtg
     5281 tcaacaagta tctggggcca tacgtgatga tgattggaaa gatgatgatc gacatgctgt
     5341 actttgtggt catcatgctg gtcgtgctca tgagtttcgg agtagcccgt caagccattc
     5401 tgcatccaga ggagaagccc tcttggaaac tggcccgaaa catcttctac atgccctact
     5461 ggatgatcta tggagaggtg tttgcagacc agatagacct ctacgccatg gaaattaatc
     5521 ctccttgtgg tgagaaccta tatgatgagg agggcaagcg gcttcctccc tgtatccccg
     5581 gcgcctggct cactccagca ctcatggcgt gctatctact ggtcgccaac atcctgctgg
     5641 tgaacctgct gattgctgtg ttcaacaata ccttctttga agtaaaatca atatccaacc
     5701 aggtgtggaa gttccagcga tatcagctga ttatgacatt tcatgacagg ccagtcctgc
     5761 ccccaccgat gatcatttta agccacatct acatcatcat tatgcgtctc agcggccgct
     5821 gcaggaaaaa gagagaaggg gaccaagagg aacgggatcg tggattgaag ctcttcctta
     5881 gcgacgagga gctaaagagg ctgcatgagt tcgaggagca gtgcgtgcag gagcacttcc
     5941 gggagaagga ggatgagcag cagtcgtcca gcgacgagcg catccgggtc acttctgaaa
     6001 gagttgaaaa tatgtcaatg gggttggaag aaatcaatga aagagaaact tttatgaaaa
     6061 cttccctgca gactgttgac cttcgacttg ctcagctaga agaattatct aacagaatgg
     6121 tgaatgctct tgaaaatctt gcgggaatcg acaggtctga cctgatccag gcacggtccc
     6181 gggcttcttc tgaatgtgag gcaacgtatc ttctccggca aagcagcatc aatagcgctg
     6241 atggctacag cttgtatcga tatcatttta acggagaaga gttattattt gaggatacat
     6301 ctctctccac gtcaccaggg acaggagtca ggaaaaaaac ctgttccttc cgtataaagg
     6361 aagagaagga cgtgaaaacg cacctagtcc cagaatgtca gaacagtctt cacctttcac
     6421 tgggcacaag cacatcagca accccagatg gcagtcacct tgcagtagat gacttaaaga
     6481 acgctgaaga gtcaaaatta ggtccagata ttgggatttc aaaggaagat gatgaaagac
     6541 agacagactc taaaaaagaa gaaactattt ccccaagttt aaataaaaca gatgtgatac
     6601 atggacagga caaatcagat gttcaaaaca ctcagctaac agtggaaacg acaaatatag
     6661 aaggcactat ttcctatccc ctggaagaaa ccaaaattac acgctatttc cccgatgaaa
     6721 cgatcaatgc ttgtaaaaca atgaagtcca gaagcttcgt ctattcccgg ggaagaaagc
     6781 tggtcggtgg ggttaaccag gatgtagagt acagttcaat cacggaccag caattgacga
     6841 cggaatggca atgccaagtt caaaagatca cgcgctctca tagcacagat attccttaca
     6901 ttgtgtcgga agctgcagtg caagctgagc ataaagagca gtttgcagat atgcaagatg
     6961 aacaccatgt cgctgaagca attcctcgaa tccctcgctt gtccctaacc attactgaca
     7021 gaaatgggat ggaaaactta ctgtctgtga agccagatca aactttggga ttcccatctc
     7081 tcaggtcaaa aagtttacat ggacatccta ggaatgtgaa atccattcag ggaaagttag
     7141 acagatctgg acatgccagt agtgtaagca gcttagtaat tgtgtctgga atgacagcag
     7201 aagaaaaaaa ggttaagaaa gagaaagctt ccacagaaac tgaatgctag aattctcgac
     7261 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     7321 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     7381 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     7441 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     7501 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     7561 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     7621 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     7681 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     7741 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     7801 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     7861 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     7921 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccgccac
     7981 catggaagat gccaagaaca tcaagaaagg ccctgccccc ttctaccccc tggaagatgg
     8041 cacagccggc gagcagctgc acaaggccat gaagagatac gccctcgtcc ctggaacaat
     8101 cgccttcacc gacgcccaca tcgaggtgga catcacctac gccgagtact tcgagatgag
     8161 cgtgcggctg gccgaggcca tgaaacgcta cggcctgaac accaaccacc ggatcgtggt
     8221 gtgcagcgag aacagcctgc agttcttcat gcccgtgctg ggcgccctgt tcatcggcgt
     8281 ggccgtggcc cctgccaacg acatctacaa cgagcgggag ctgctgaaca gcatgggcat
     8341 cagccagccc accgtggtgt tcgtgagcaa gaagggcctg cagaaaatcc tgaacgtgca
     8401 gaagaagctg cccatcatcc agaaaatcat catcatggac agcaagaccg actaccaggg
     8461 cttccagagc atgtacacct tcgtgaccag ccacctgccc cctggcttca acgagtacga
     8521 cttcgtgccc gagagcttcg acagggacaa gaccatcgcc ctgatcatga acagcagcgg
     8581 cagcaccggc ctgcctaaag gcgtggccct gcctcaccgg accgcctgcg tgcggttcag
     8641 ccacgcccgg gaccccatct tcggcaacca gatcatcccc gacaccgcca tcctgagcgt
     8701 ggtgcccttc caccacggct tcggcatgtt caccaccctg ggctacctga tctgcggctt
     8761 ccgggtggtg ctgatgtatc ggttcgagga agagctgttc ctgcggagcc tgcaggacta
     8821 caagatccag agcgccctgc tggtgcccac cctgttcagc tttttcgcca agagcaccct
     8881 gatcgacaag tacgacctga gcaacctgca cgagatcgcc agcggcggag cccccctgag
     8941 caaagaagtg ggcgaagccg tcgccaagcg gttccacctg cccggcatcc ggcagggcta
     9001 tggcctgacc gagaccacaa gcgccattct gatcaccccc gagggcgacg acaagcctgg
     9061 cgccgtgggc aaggtggtgc ctttcttcga ggccaaggtg gtggacctgg acaccggcaa
     9121 gaccctgggc gtgaaccagc ggggcgagct gtgcgtgagg ggccccatga tcatgagcgg
     9181 ctacgtgaac aaccccgagg ccaccaacgc cctgattgac aaggacggct ggctgcacag
     9241 cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag
     9301 cctgatcaag tacaagggct accaggtggc cccagccgag ctggaaagca tcctgctgca
     9361 gcaccccaac atcttcgacg ccggggtggc cggcctgccc gacgatgacg ccggcgagct
     9421 gcctgccgcc gtggtggtcc tcgaacatgg caaaaccatg accgagaaag aaatcgtgga
     9481 ctacgtggcc agccaggtga ccaccgccaa gaagctgaga ggcggcgtcg tgtttgtgga
     9541 cgaggtgccc aagggcctga ccggcaagct ggacgcccgg aagatccggg agatcctgat
     9601 taaggccaag aagggcggca agatcgccgt gggctccggc cagtgcacca actacgccct
     9661 gctgaagctg gccggcgacg tggagagcaa ccctggccct ttcgaaatgg tgtccaaggg
     9721 cgaagaggac aacatggcca tcatcaaaga gtttatgcgg ttcaaggtgc acatggaagg
     9781 cagcgtgaac ggccacgagt tcgagatcga gggcgagggc gaaggcaggc cctacgaggg
     9841 cacccagacc gccaagctga aggtgaccaa gggcggacct ctgcccttcg cctgggacat
     9901 cctgagcccc cagttcatgt acggcagcaa ggcctacgtg aagcaccccg ccgacatccc
     9961 cgactacctg aagctgtcct tccccgaggg cttcaagtgg gagcgggtga tgaacttcga
    10021 ggacggcggg gtggtgaccg tgacccagga tagctccctg caggacggcg agttcatcta
    10081 caaggtgaaa ctgcggggca ccaacttccc cagcgacggc cccgtgatgc agaaaaagac
    10141 aatgggctgg gaggccagca gcgagcggat gtaccccgag gatggcgccc tgaagggcga
    10201 gatcaagcag cggctgaagc tgaaggacgg cggccactac gacgccgagg tgaaaaccac
    10261 ctacaaggcc aaaaagcccg tgcagctgcc tggcgcctac aacgtgaaca tcaagctgga
    10321 tatcaccagc cacaacgagg actacaccat cgtggagcag tacgagcggg ccgagggcag
    10381 acacagcacc ggcggcatgg acgagctgta caagctcagc ggcagcggcg agggcagagg
    10441 cagcctgctg acctgcggcg atgtggagga aaacccaggc ccaatgaccg agtacaagcc
    10501 taccgtgagg ctggccaccc gggacgacgt gcccagagcc gtgaggacac tggccgccgc
    10561 cttcgccgac taccccgcca cccggcacac cgtggacccc gaccggcaca tcgagcgggt
    10621 gaccgagctg caggaactgt tcctgaccag agtgggcctg gatatcggca aagtgtgggt
    10681 ggccgacgac ggagccgccg tggccgtgtg gaccaccccc gagtccgtgg aggccggagc
    10741 cgtgtttgcc gagatcggcc ccaggatggc cgagctgtcc ggcagcaggc tggccgccca
    10801 gcagcagatg gaaggcctgc tggcccctca ccggcccaaa gagcccgcct ggttcctggc
    10861 caccgtgggc gtgagccccg accaccaggg caagggcctg ggcagcgccg tggtgctgcc
    10921 aggcgtggaa gccgccgaga gggccggagt gcccgccttc ctggaaacca gcgcccccag
    10981 gaacctgccc ttctacgagc ggctgggctt taccgtgacc gccgacgtgg aggtgccaga
    11041 gggccccagg acctggtgca tgacccggaa gccaggcgcc tgagaattgg gtacctttaa
    11101 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11161 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    11221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    11281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    11341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
    11401 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
    11461 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    11521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    11581 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
    11641 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    11701 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11761 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    11821 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    11881 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    11941 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
    12001 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    12061 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    12121 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    12181 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    12241 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    12301 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    12361 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    12421 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    12481 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    12541 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    12601 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    12661 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    12721 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    12781 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    12841 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    12901 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    12961 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    13021 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    13081 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    13141 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    13201 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    13261 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    13321 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    13381 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    13441 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    13501 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    13561 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    13621 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    13681 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    13741 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    13801 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    13861 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    13921 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    13981 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    14041 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    14101 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    14161 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    14221 cttttgctca catgttcttt cctgcgttat cccctgat
//
