LOCUS       sequence-194321-        2646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chimeric spike resembling fungal ITS region to be used in 
            metagenomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2646)
  TITLE     metagenomic_spikes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSPIKE-F
FEATURES             Location/Qualifiers
     source          1..2646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     223..245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     237..254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     238..254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     265..284
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        265..283
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(734..751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(844..1432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(924..943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1603..2394,2395..2463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2226..2245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2464..2568)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
       61 accgagatag ggttgagtgg ccgctacagg gcgctcccat tcgccattca ggctgcgcaa
      121 ctgttgggaa gggcgtttcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      181 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      241 aaacgacggc cagtgagcgc gacgtaatac gactcactat agggcgaatt ggcggaaggc
      301 cgtcaaggcc acgtgtcttg tccagagctc cttggtcatt tagaggaagt aatcccttgt
      361 ctcctaaaaa acaaagattt actatgcaca gaggaacgtc tatctaacgg ttggtatctt
      421 gaatgctcgg tcccttttgt cattccggat taattcattt ccctcattca caagcttgcg
      481 aagtctatat tgatatatga atgcaatcta gaagagggca cttaaaatta gcagtagtta
      541 atattttaaa ctccatttgg tttattcgtt acgagactga ttgcatcgat gaagaacgca
      601 gcggtacctg gagcacaaga ctggcctcat gggccttccg ctcactgccc gctttccagt
      661 cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg
      721 cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg
      781 tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1741 gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2581 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2641 taaatc
//
