LOCUS       sequence-194365-         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Reverse Transcriptase gene (3'-5' exonuclease 
            deficient mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Ellefson JW, Gollihar J, Shroff R, Shivram H, Iyer VR, Ellington AD
  TITLE     Synthetic evolutionary origin of a proofreading reverse 
            transcriptase.
  JOURNAL   Science. 2016 Jun 24;352(6293):1590-3. doi: 10.1126/science.aaf5409.
  PUBMED    27339990
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-24- 24"; volume: "352"; 
            issue: "6293"; pages: "1590-3"
COMMENT     Sequence Label: pET_RTX_(exo-)
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             171..179
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             578..595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgttcgtga caaagaagaa atacgcggta attgacgagg aaggcaagat cacaactcgt
       61 ggcttggaaa ttgttcgtcg cgattggagc gagatcgcaa aggagaccca agctcgtgtg
      121 ttggaggccc tcctgaagga tggtgacgtc gaaaaagccg tacgcatcgt taaggaggtt
      181 acagagaagc ttagcaagta tgaggtccca ccagagaaac ttgttattca taaacaaatc
      241 actcgcgacc ttaaagacta taaggccact ggtccacacg tcgccgtagc aaagcggctt
      301 gcggctcggg gcgtcaagat tcggccaggc acggttatta gttacatcgt cctcaaaggc
      361 tcaggccgga ttgttgatcg cgcgattcca tttgatgaat ttgatccgac gaagcataaa
      421 tatgatgcgg aatattacat tgaaaaacag gttctgccgg cggtggagcg catcttacgt
      481 gcgttcggct atcgcaagga ggatttgcgg taccagaaaa ctcgtcaagt cggtttgagt
      541 gcctggctga agccgaaagg tacctgataa ctccagccat caccatcacc atcactgata
      601 agcggccgca ttgatccggc tgc
//
