LOCUS       sequence-194392-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP under WT T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pACYC-GFPM2
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     512..532
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      583..630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      633..680
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc gcgtatggtc
       61 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      121 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      181 cacgtgctga agtcaaattt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      241 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      301 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      361 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      421 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      481 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      541 ggattacaca tggcatggat gaactataca aatagaatta gactagcata accccttggg
      601 gcctctaaac gggtcttgag gggttttttg cactagcata accccttggg gcctctaaac
      661 gggtcttgag gggttttttg cgaattcgat ctcaattgga tattccactc gagtctggta
      721 aagaaaccgc tgctgcgaaa tttgaacgcc agcac
//
