LOCUS       sequence-194472-        8936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EIF3M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7137 pHAGE-P-CMVt-N-HA-GAW-EIF3M
FEATURES             Location/Qualifiers
     source          1..8936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1034..1055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1070..1075,1076..1093,1094..1100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1108..1124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1113..1135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1132..1148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1132..1148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1198..1217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1409..2042
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2089..2214
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2711..2944
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3129..3173
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3466..3583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3635..3938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3939..4142
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4093..4113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4144..4162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4165..4183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4193..4217
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4304..4313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4316..4342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4358..4382
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5505..5529)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5548..6047
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5583..5606)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5980..5999
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6068..6667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6068..6087)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6564..6584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6734..7322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6787..6807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7205..7216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7397..7630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7749..7767)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7835..7939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7940..8008,8009..8800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8158..8177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
      901 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      961 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
     1021 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     1081 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1141 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1201 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     1261 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     1321 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     1381 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     1441 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     1501 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     1561 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     1621 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     1681 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     1741 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     1801 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     1861 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     1921 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     1981 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2041 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2101 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2161 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2221 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2281 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2341 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     2401 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     2461 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     2521 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     2581 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     2641 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     2701 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     2761 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     2821 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     2881 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     2941 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     3001 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     3061 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     3121 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     3181 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     3241 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     3301 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     3361 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     3421 ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg
     3481 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3541 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     3601 cagcagagat ccagtttgga ctagtggagt tccgcgttac ataacttacg gtaaatggcc
     3661 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3721 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3781 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3841 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3901 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3961 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4021 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4081 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4141 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
     4201 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     4261 accgggaccg atccagcctc cggactctag cgtttaaacg ctagccacca tggcgtaccc
     4321 atacgatgtt ccagattacg ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg
     4381 catgagcgtc ccggccttca tcgacatcag tgaagaagat caggctgctg agcttcgtgc
     4441 ttatctgaaa tctaaaggag ctgagatttc agaagagaac tcggaaggtg gacttcatgt
     4501 tgatttagct caaattattg aagcctgtga tgtgtgtctg aaggaggatg ataaagatgt
     4561 tgaaagtgtg atgaacagtg tggtatccct actcttgatc ctggaaccag acaagcaaga
     4621 agctttgatt gaaagcctat gtgaaaagct ggtcaaattt cgcgaaggtg aacgcccgtc
     4681 tctgagactg cagttgttaa gcaacctttt ccacgggatg gataagaata ctcctgtaag
     4741 atacacagtg tattgcagcc ttattaaagt ggcagcatct tgtggggcca tccagtacat
     4801 cccaactgag ctggatcaag ttagaaaatg gatttctgac tggaatctca ccactgaaaa
     4861 aaagcacacc cttttaagac tactttatga ggcacttgtg gattgtaaga agagtgatgc
     4921 tgcttcaaaa gtcatggtgg aattgctcgg aagttacaca gaggacaatg cttcccaggc
     4981 tcgagttgat gcccacaggt gtattgtacg agcattgaaa gatccaaatg catttctttt
     5041 tgaccacctt cttactttaa aaccagtcaa gtttttggaa ggcgagctta ttcatgatct
     5101 tttaaccatt tttgtgagtg ctaaattggc atcatatgtc aagttttatc agaataataa
     5161 agacttcatt gattcacttg gcctgttaca tgaacagaat atggcaaaaa tgagactact
     5221 tacttttatg ggaatggcag tagaaaataa ggaaatttct tttgacacaa tgcagcaaga
     5281 acttcagatt ggagctgatg atgttgaagc atttgttatt gacgccgtaa gaactaaaat
     5341 ggtctactgc aaaattgatc agacccagag aaaagtagtt gtcagtcata gcacacatcg
     5401 gacatttgga aaacagcagt ggcaacaact gtatgacaca cttaatgcct ggaaacaaaa
     5461 tctgaacaaa gtgaaaaaca gccttttgag tctttctgat acctgcccaa ctttcttgta
     5521 caaagtggtt cgataacgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     5581 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     5641 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     5701 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     5761 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     5821 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     5881 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     5941 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     6001 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     6061 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6121 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6181 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6241 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6301 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6361 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6421 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6481 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6541 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6601 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6661 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     6721 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7141 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7321 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7381 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7441 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7621 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7681 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
     7741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7801 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8161 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgag
//
