LOCUS       sequence-19452-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 362 pCS Cherry DEST
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..739
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(177..195)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     445..464
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     639..662
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    752..876
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccatcgattc gaattcaagg ctagcgccac catggtgagc aagggcgagg aggacaacat
       61 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggccg
      121 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      181 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      241 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      301 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      361 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      421 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      481 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
      541 gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca aggccaagaa
      601 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ctggacatca cctcccacaa
      661 cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgg
      721 catggacgag ctgtacaaga gatctcgaat cacaagtttg tacaaaaaag ctgaacgaga
      781 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      841 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcatta
//
