LOCUS       sequence-194612-         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SpyCatcher002 with a peptide tag enabling 
            site-specific biotinylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil 
            Anuar INA
  TITLE     Evolving accelerated amidation by SpyTag/SpyCatcher to analyze 
            membrane dynamics.
  JOURNAL   Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623.
  PUBMED    29024296
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623."
COMMENT     Sequence Label: pDEST14-Avitag-SpyCatcher002
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..81
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             106..126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             133..471
                     /codon_start=1
                     /product="improved SpyCatcher variant that shows rapid 
                     reaction with SpyTag002 (Keeble et al., 2017)"
                     /label=SpyCatcher002
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     EATKGDAHT"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ggcagcggcc tgaacgatat ttttgaagcg
       61 cagaaaattg aatggcatga aggcgattac gacatcccaa cgaccgaaaa cctgtatttt
      121 cagggcgcca tggtaaccac cttatcaggt ttatcaggtg agcaaggtcc gtccggtgat
      181 atgacaactg aagaagatag tgctacccat attaaattct caaaacgtga tgaggacggc
      241 cgtgagttag ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca
      301 tggatttcag atggacatgt gaaggatttc tacctgtatc caggaaaata tacatttgtc
      361 gaaaccgcag caccagacgg ttatgaggta gcaactgcta ttacctttac agttaatgag
      421 caaggtcagg ttactgtaaa tggcgaagca actaaaggtg acgctcatac tggatccagt
      481 ggtagctaa
//
