LOCUS       sequence-194734-       18116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for CAG promoter constitutive expression of 
            dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53
            shRNA expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 18116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: pCXLE-dCas9VPP300-T2A-GFP-shP53
FEATURES             Location/Qualifiers
     source          1..18116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1752..1778
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1782..1802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1812..1835,1836..1838,1839..4394,4395..4397,
                     4398..5912)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1812..1835,1836..1838,1839..4325,4326..4328,
                     4329..5912)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5916..5936
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5973..6083
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6135..6245
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6297..6407
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6459..6569
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6618..8468
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      8232..8241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8484..8537
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8544..8546,8547..8549,8550..9263)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8544..8546,8547..8549,8550..9260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8850..8869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9197..9218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9301..9889
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9354..9374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9772..9783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9979..9998)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10044..10099
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10098..10117)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10460..10476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10460..10476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10473..10495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10484..10500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10508..10514,10515..10532,10533..10538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10553..10574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    10663..10696
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             11157..13082
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    13250..13283
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      13470..15259
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        15730..16043
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     15962..15982
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(16194..16211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16365..16953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16445..16464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17124..17915,17916..17984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17747..17766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17985..18089)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaccg
     1741 gtgccaccat gtacccatac gatgttccag attacgcttc gccgaagaaa aagcgcaagg
     1801 tcgaagcgtc cgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct
     1861 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     1921 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     1981 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     2041 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     2101 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     2161 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     2221 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     2281 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     2341 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     2401 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     2461 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     2521 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     2581 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     2641 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     2701 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     2761 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     2821 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     2881 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     2941 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     3001 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     3061 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     3121 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     3181 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     3241 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     3301 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     3361 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     3421 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     3481 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     3541 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     3601 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     3661 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     3721 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     3781 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     3841 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     3901 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     3961 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     4021 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     4081 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     4141 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     4201 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     4261 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     4321 tcgacgccat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     4381 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     4441 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     4501 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     4561 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     4621 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     4681 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     4741 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     4801 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     4861 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     4921 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     4981 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     5041 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     5101 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     5161 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     5221 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     5281 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     5341 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     5401 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     5461 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     5521 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     5581 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     5641 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     5701 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     5761 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     5821 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     5881 cacggatcga cctgtctcag ctgggaggcg acagccccaa gaagaagaga aaggtggagg
     5941 ccagcctcga gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca
     6001 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     6061 acgattttga ccttgacatg ctccccgggt cactacccag cgctagcgtc gagtgcgaaa
     6121 gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     6181 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     6241 tgctccccgg gtcactaccc agcgctagcg tcgagtgcga aagcggcggg ccggccgacg
     6301 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     6361 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtcactac
     6421 ccagcgctag cgtcgagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact
     6481 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     6541 ctcttgacga ttttgacctt gacatgctcc ccgggtcact acccagcgct agcgtcgagt
     6601 tcgaaggcag cggcgggatt ttcaaaccag aagaactacg acaggcactg atgccaactt
     6661 tggaggcact ttaccgtcag gatccagaat cccttccctt tcgtcaacct gtggaccctc
     6721 agcttttagg aatccctgat tactttgata ttgtgaagag ccccatggat ctttctacca
     6781 ttaagaggaa gttagacact ggacagtatc aggagccctg gcagtatgtc gatgatattt
     6841 ggcttatgtt caataatgcc tggttatata accggaaaac atcacgggta tacaaatact
     6901 gctccaagct ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa agccttggat
     6961 actgttgtgg cagaaagttg gagttctctc cacagacact gtgttgctac ggcaaacagt
     7021 tgtgcacaat acctcgtgat gccacttatt acagttacca gaacaggtat catttctgtg
     7081 agaagtgttt caatgagatc caaggggaga gcgtttcttt gggggatgac ccttcccagc
     7141 ctcaaactac aataaataaa gaacaatttt ccaagagaaa aaatgacaca ctggatcctg
     7201 aactgtttgt tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt gtccttcacc
     7261 atgagatcat ctggcctgct ggattcgtct gtgatggctg tttaaagaaa agtgcacgaa
     7321 ctaggaaaga aaataagttt tctgctaaaa ggttgccatc taccagactt ggcacctttc
     7381 tagagaatcg tgtgaatgac tttctgaggc gacagaatca ccctgagtca ggagaggtca
     7441 ctgttagagt agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc atgaaagcaa
     7501 ggtttgtgga cagtggagag atggcagaat cctttccata ccgaaccaaa gccctctttg
     7561 cctttgaaga aattgatggt gttgacctgt gcttctttgg catgcatgtt caagagtatg
     7621 gctctgactg ccctccaccc aaccagagga gagtatacat atcttacctc gatagtgttc
     7681 atttcttccg tcctaaatgc ttgaggactg cagtctatca tgaaatccta attggatatt
     7741 tagaatatgt caagaaatta ggttacacaa cagggcatat ttgggcatgt ccaccaagtg
     7801 agggagatga ttatatcttc cattgccatc ctcctgacca gaagataccc aagcccaagc
     7861 gactgcagga atggtacaaa aaaatgcttg acaaggctgt atcagagcgt attgtccatg
     7921 actacaagga tatttttaaa caagctactg aagatagatt aacaagtgca aaggaattgc
     7981 cttatttcga gggtgatttc tggcccaatg ttctggaaga aagcattaag gaactggaac
     8041 aggaggaaga agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca gatgtgacca
     8101 agggagacag caaaaatgct aaaaagaaga ataataagaa aaccagcaaa aataagagca
     8161 gcctgagtag gggcaacaag aagaaacccg ggatgcccaa tgtatctaac gacctctcac
     8221 agaaactata tgccaccatg gagaagcata aagaggtctt ctttgtgatc cgcctcattg
     8281 ctggccctgc tgccaactcc ctgcctccca ttgttgatcc tgatcctctc atcccctgcg
     8341 atctgatgga tggtcgggat gcgtttctca cgctggcaag ggacaagcac ctggagttct
     8401 cttcactccg aagagcccag tggtccacca tgtgcatgct ggtggagctg cacacgcaga
     8461 gccaggacgt cgacggctcc ggagagggca gaggaagtct gctaacatgc ggtgacgtcg
     8521 aggagaatcc tggcccaatc gatatggtga gcaagggcga ggagctgttc accggggtgg
     8581 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     8641 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     8701 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     8761 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     8821 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     8881 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     8941 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     9001 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     9061 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     9121 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     9181 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     9241 gcatggacga gctgtacaag taatttaaag aattcaagct tcggggacta gtcatatgat
     9301 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9361 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9421 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9481 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9541 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9601 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9661 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     9721 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9781 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9841 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcggtaaatt
     9901 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     9961 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
    10021 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
    10081 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
    10141 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
    10201 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
    10261 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
    10321 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
    10381 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
    10441 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    10501 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    10561 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    10621 gccagcggat ctcaattccg atcatattca ataaccctta atataacttc gtataatgta
    10681 tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct taggatcaat
    10741 tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac ccagcttttc
    10801 ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg
    10861 agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg cctggcttga
    10921 ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc gagtaattgg
    10981 tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc tgtctgacag
    11041 cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc tgcactccct
    11101 gtattcactg agcgtcgtct aataaagatg tctattgatc tcttttagtg tgaatcatgt
    11161 ctgacgaggg gccaggtaca ggacctggaa atggcctagg agagaaggga gacacatctg
    11221 gaccagaagg ctccggcggc agtggacctc aaagaagagg gggtgataac catggacgag
    11281 gacggggaag aggacgagga cgaggaggcg gaagaccagg agccccgggc ggctcaggat
    11341 cagggccaag acatagagat ggtgtccgga gaccccaaaa acgtccaagt tgcattggct
    11401 gcaaagggac ccacggtgga acaggagcag gagcaggagc gggaggggca ggagcaggag
    11461 gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag
    11521 gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
    11581 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
    11641 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag
    11701 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
    11761 caggagcagg aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag
    11821 gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
    11881 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag
    11941 gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag
    12001 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
    12061 gggcaggagc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggagggg
    12121 caggagcagg aggtggaggc cggggtcgag gaggcagtgg aggccggggt cgaggaggta
    12181 gtggaggccg gggtcgagga ggtagtggag gccgccgggg tagaggacgt gaaagagcca
    12241 gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg acgtggagaa aagaggccca
    12301 ggagtcccag tagtcagtca tcatcatccg ggtctccacc gcgcaggccc cctccaggta
    12361 gaaggccatt tttccaccct gtaggggaag ccgattattt tgaataccac caagaaggtg
    12421 gcccagatgg tgagcctgac gtgcccccgg gagcgataga gcagggcccc gcagatgacc
    12481 caggagaagg cccaagcact ggaccccggg gtcagggtga tggaggcagg cgcaaaaaag
    12541 gagggtggtt tggaaagcat cgtggtcaag gaggttccaa cccgaaattt gagaacattg
    12601 cagaaggttt aagagctctc ctggctagga gtcacgtaga aaggactacc gacgaaggaa
    12661 cttgggtcgc cggtgtgttc gtatatggag gtagtaagac ctccctttac aacctaaggc
    12721 gaggaactgc ccttgctatt ccacaatgtc gtcttacacc attgagtcgt ctcccctttg
    12781 gaatggcccc tggacccggc ccacaacctg gcccgctaag ggagtccatt gtctgttatt
    12841 tcatggtctt tttacaaact catatatttg ctgaggtttt gaaggatgcg attaaggacc
    12901 ttgttatgac aaagcccgct cctacctgca atatcagggt gactgtgtgc agctttgacg
    12961 atggagtaga tttgcctccc tggtttccac ctatggtgga aggggctgcc gcggagggtg
    13021 atgacggaga tgacggagat gaaggaggtg atggagatga gggtgaggaa gggcaggagt
    13081 gatgtaactt gttaggagac gccctcaatc gtattaaaag ccgtgtattc ccccgcacta
    13141 aagaataaat ccccagtaga catcatgcgt gctgttggtg tatttctggc catctgtctt
    13201 gtcaccattt tcgtcctccc aacatggggc aattgccgga acccttaata taacttcgta
    13261 taatgtatgc tatacgaagt tattaggtcc ctcgaagagg ttcactagcg gatctcaatt
    13321 gggcataccc atgttgtcac gtcactcagc tccgcgctca acaccttctc gcgttggaaa
    13381 acattagcga catttacctg gtgagcaatc agacatgcga cggctttagc ctggcctcct
    13441 taaattcacc taagaatggg agcaaccagc aggaaaagga caagcagcga aaattcacgc
    13501 ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta ctgggtatca
    13561 tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat taggatagca
    13621 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
    13681 tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
    13741 tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
    13801 tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg
    13861 ctacccagat ataaattagg atagcatata ctaccctaat ctctattagg atagcatatg
    13921 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
    13981 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
    14041 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
    14101 ctacccagat atagattagg atagcatatg ctatccagat atttgggtag tatatgctac
    14161 ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg accaacaacc
    14221 ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc agcaatcgcg
    14281 cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc attagtggtt
    14341 ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca agttattaca
    14401 cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg tgcccccact
    14461 ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca ttggcgtgga
    14521 gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc tgtcggcgtc
    14581 cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg tcttggtccc
    14641 tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat gtgttgccca
    14701 tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc accccatgga
    14761 tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta ttgcccaagg
    14821 ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc cccgtccaaa
    14881 ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata caccttactg
    14941 ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag caggcgaaga
    15001 ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg actaaaatgg
    15061 ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc tcatggggtg
    15121 ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc atatgcttcc
    15181 cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac tactacccgg
    15241 gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta ttgctaatgc
    15301 cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg ttggtgtagc
    15361 ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg tgtaagagct
    15421 tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact gcaaagtctg
    15481 ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata aggatgtcaa
    15541 ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc
    15601 cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg
    15661 gcagagatgt cgtagtcagg tttagttcgt ccggggcggg gcatcgatcc tctagagtcg
    15721 acgctagcgg atccgacgcc gccatctcta ggcccgcgcc ggccccctcg cacagacttg
    15781 tgggagaagc tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac
    15841 tatagaggct taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat
    15901 tttacatgat aggcttggat ttctataaga gatacaaata ctaaattatt attttaaaaa
    15961 acagcacaaa aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataaat
    16021 atcccttgga gaaaagcctt gtttgggccg actccagtgg taatctactt caagagagta
    16081 gattaccact ggagtctttt ttggaattcc tgcagcccgg gggatccgct gcattaatga
    16141 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    16201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    16261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    16321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    16381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    16441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    16501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    16561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    16621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    16681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    16741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    16801 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    16861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    16921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    16981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    17041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    17101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    17161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    17221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    17281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    17341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    17401 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    17461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    17521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    17581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    17641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    17701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    17761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    17821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    17881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    17941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    18001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    18061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
