LOCUS       sequence-194737-       13412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs 
            targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes 
            PGK-puro selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 13412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: GG-EBNA-OMKSL-PP
FEATURES             Location/Qualifiers
     source          1..13412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(190..209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     309..331
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(369..387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        455..559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(560..628,629..1420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(778..797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1591..2179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2080..2099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2467..2488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2503..2508,2509..2526,2527..2533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2541..2557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2546..2568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2565..2581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2600..2619
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2600..2618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2685..2701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2702..2950
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2971..3046
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3078..3094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3095..3343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3365..3440
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3452..3468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3472..3488
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3489..3737
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3759..3834
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3866..3882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3883..4131
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4153..4228
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4260..4276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4277..4525
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4547..4622
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4780..4813
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5155..5171
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5172..5420
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5442..5517
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     5549..5565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5566..5814
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5836..5911
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(5923..5939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5943..5959
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5960..6208
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6230..6305
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     6337..6353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6354..6602
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6624..6699
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     6731..6747
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6748..6996
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        7018..7093
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7251..7284
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7345..7842
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     7775..7794
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7882..8481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7882..7901)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8378..8398
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8930..10855
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    11023..11056
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      11243..13032
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
       61 ggcatcgatc ctctagtaag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
      181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      241 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      301 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      421 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      781 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      961 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1021 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1081 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1141 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1201 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1261 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1321 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1381 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1441 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2221 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2281 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2341 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2401 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2461 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2521 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2581 ccatgattac gccaagctct aatacgactc actataggga gacaagcttg catgcctgca
     2641 ggtcgactct agaggatctc gagctcaagc ttcgaattcc ccctgtaaaa cgacggccag
     2701 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2761 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2821 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2881 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2941 acgaaacacc ggggggagaa actgaggcga gttttagagc tagaaatagc aagttaaaat
     3001 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttcatggtc
     3061 atagctgttt cctcatggta aaacgacggc cagtgagggc ctatttccca tgattccttc
     3121 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     3181 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     3241 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     3301 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcccttt ataatgcgag
     3361 ggtcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     3421 aagtggcacc gagtcggtgc tttttttcat ggtcatagct gtttcctgga cgtaaaacga
     3481 cggccagtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     3541 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     3601 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     3661 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3721 ggaaaggacg aaacaccggc tgccatagca acgatggagt tttagagcta gaaatagcaa
     3781 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     3841 tcatggtcat agctgtttcc tccaggtaaa acgacggcca gtgagggcct atttcccatg
     3901 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     3961 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     4021 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     4081 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggcacagt
     4141 gccagaggtc tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     4201 acttgaaaaa gtggcaccga gtcggtgctt tttttcatgg tcatagctgt ttccttgttg
     4261 taaaacgacg gccagtgagg gcctatttcc catgattcct tcatatttgc atatacgata
     4321 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
     4381 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     4441 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     4501 tatcttgtgg aaaggacgaa acaccgtctg tgggggacct gcactggttt tagagctaga
     4561 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     4621 gctttttttc atggtcatag ctgtttccta acgagcggcc gcaggaaccc ctagtgatgg
     4681 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4741 cccgacgccc aattccgatc atattcaata acccttaata taacttcgta taatgtatgc
     4801 tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagcttag gatctcgacc
     4861 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4921 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4981 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5041 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5101 tggaagtagc acgtctcact agaggatctc gagctcaagc ttcgaattcc ccctgtaaaa
     5161 cgacggccag tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     5221 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     5281 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     5341 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     5401 tgtggaaagg acgaaacacc ggtgtcagag accggagttg tgttttagag ctagaaatag
     5461 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     5521 ttttcatggt catagctgtt tcctcatggt aaaacgacgg ccagtgaggg cctatttccc
     5581 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     5641 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     5701 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     5761 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggtggc
     5821 tggcaggctg gctctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     5881 tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc tgtttcctgg
     5941 acgtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     6001 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     6061 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     6121 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     6181 atatatcttg tggaaaggac gaaacaccgc ggggtactca agtcttctag ttttagagct
     6241 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     6301 ggtgcttttt ttcatggtca tagctgtttc ctccaggtaa aacgacggcc agtgagggcc
     6361 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     6421 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     6481 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     6541 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     6601 ccgtaattat ctgcccgggg ggtgttttag agctagaaat agcaagttaa aataaggcta
     6661 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcatg gtcatagctg
     6721 tttccttgtt gtaaaacgac ggccagtgag ggcctatttc ccatgattcc ttcatatttg
     6781 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     6841 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     6901 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     6961 ttggctttat atatcttgtg gaaaggacga aacaccgcaa aacccggcag cgaggctgtt
     7021 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     7081 accgagtcgg tgcttttttt catggtcata gctgtttcct aacgagcggc cgcaggaacc
     7141 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     7201 accaaaggtc gcccgacgcc caattccgat catattcaat aacccttaat ataacttcgt
     7261 ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca gccaagctta
     7321 ggatctcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     7381 catgcgcttt agcagccccg ctggcacttg gcgctacaca agtggcctct ggcctcgcac
     7441 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     7501 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     7561 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     7621 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     7681 ctgggctcag cagctgggaa gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     7741 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     7801 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat
     7861 ctcgagcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7921 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7981 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8041 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8101 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8161 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8221 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8281 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8341 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     8401 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8461 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     8521 gcacgacccc atgcatcgat aagctgatcc tcacaggccg cacccagctt ttcttccgtt
     8581 gccccagtag catctctgtc tggtgacctt gaagaggaag aggaggggtc ccgagaatcc
     8641 ccatccctac cgtccagcaa aaagggggac gaggaatttg aggcctggct tgaggctcag
     8701 gacgcaaatc ttgaggatgt tcagcgggag ttttccgggc tgcgagtaat tggtgatgag
     8761 gacgaggatg gttcggagga tggggaattt tcagacctgg atctgtctga cagcgaccat
     8821 gaaggggatg agggtggggg ggctgttgga gggggcagga gtctgcactc cctgtattca
     8881 ctgagcgtcg tctaataaag atgtctattg atctctttta gtgtgaatca tgtctgacga
     8941 ggggccaggt acaggacctg gaaatggcct aggagagaag ggagacacat ctggaccaga
     9001 aggctccggc ggcagtggac ctcaaagaag agggggtgat aaccatggac gaggacgggg
     9061 aagaggacga ggacgaggag gcggaagacc aggagccccg ggcggctcag gatcagggcc
     9121 aagacataga gatggtgtcc ggagacccca aaaacgtcca agttgcattg gctgcaaagg
     9181 gacccacggt ggaacaggag caggagcagg agcgggaggg gcaggagcag gaggggcagg
     9241 agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggaggggc
     9301 aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc
     9361 aggaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg
     9421 aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc
     9481 aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc
     9541 aggaggggca ggaggggcag gaggggcagg agcaggaggg gcaggagcag gaggaggggc
     9601 aggaggggca ggaggggcag gagcaggagg ggcaggagca ggaggggcag gagcaggagg
     9661 ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg
     9721 aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc
     9781 aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg
     9841 agcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag gggcaggagc
     9901 aggaggtgga ggccggggtc gaggaggcag tggaggccgg ggtcgaggag gtagtggagg
     9961 ccggggtcga ggaggtagtg gaggccgccg gggtagagga cgtgaaagag ccaggggggg
    10021 aagtcgtgaa agagccaggg ggagaggtcg tggacgtgga gaaaagaggc ccaggagtcc
    10081 cagtagtcag tcatcatcat ccgggtctcc accgcgcagg ccccctccag gtagaaggcc
    10141 atttttccac cctgtagggg aagccgatta ttttgaatac caccaagaag gtggcccaga
    10201 tggtgagcct gacgtgcccc cgggagcgat agagcagggc cccgcagatg acccaggaga
    10261 aggcccaagc actggacccc ggggtcaggg tgatggaggc aggcgcaaaa aaggagggtg
    10321 gtttggaaag catcgtggtc aaggaggttc caacccgaaa tttgagaaca ttgcagaagg
    10381 tttaagagct ctcctggcta ggagtcacgt agaaaggact accgacgaag gaacttgggt
    10441 cgccggtgtg ttcgtatatg gaggtagtaa gacctccctt tacaacctaa ggcgaggaac
    10501 tgcccttgct attccacaat gtcgtcttac accattgagt cgtctcccct ttggaatggc
    10561 ccctggaccc ggcccacaac ctggcccgct aagggagtcc attgtctgtt atttcatggt
    10621 ctttttacaa actcatatat ttgctgaggt tttgaaggat gcgattaagg accttgttat
    10681 gacaaagccc gctcctacct gcaatatcag ggtgactgtg tgcagctttg acgatggagt
    10741 agatttgcct ccctggtttc cacctatggt ggaaggggct gccgcggagg gtgatgacgg
    10801 agatgacgga gatgaaggag gtgatggaga tgagggtgag gaagggcagg agtgatgtaa
    10861 cttgttagga gacgccctca atcgtattaa aagccgtgta ttcccccgca ctaaagaata
    10921 aatccccagt agacatcatg cgtgctgttg gtgtatttct ggccatctgt cttgtcacca
    10981 ttttcgtcct cccaacatgg ggcaattgcc ggaaccctta atataacttc gtataatgta
    11041 tgctatacga agttattagg tccctcgaag aggttcacta gcggatctca attgggcata
    11101 cccatgttgt cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag
    11161 cgacatttac ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc
    11221 acctaagaat gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg
    11281 ggaggtggcg gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg
    11341 actgtatatg catgaggata gcatatgcta cccggataca gattaggata gcatatacta
    11401 cccagatata gattaggata gcatatgcta cccagatata gattaggata gcctatgcta
    11461 cccagatata aattaggata gcatatacta cccagatata gattaggata gcatatgcta
    11521 cccagatata gattaggata gcctatgcta cccagatata gattaggata gcatatgcta
    11581 cccagatata gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca
    11641 gatataaatt aggatagcat atactaccct aatctctatt aggatagcat atgctacccg
    11701 gatacagatt aggatagcat atactaccca gatatagatt aggatagcat atgctaccca
    11761 gatatagatt aggatagcct atgctaccca gatataaatt aggatagcat atactaccca
    11821 gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca
    11881 gatatagatt aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc
    11941 aacattagcc caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct
    12001 tggcgctcag gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat
    12061 cttggcccgc ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg
    12121 caagtggttt gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat
    12181 tttacagtcc aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat
    12241 ttcaaaaaaa agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt
    12301 ttaattttcg ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc
    12361 tttccccttg ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg
    12421 gacacatctt aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat
    12481 aaattcgtgt gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat
    12541 tgttaaagat attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt
    12601 gagggttata ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt
    12661 ctgggggcgt cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa
    12721 ctcagcagtt attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga
    12781 gagttcactg cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta
    12841 ccctcgtgga atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata
    12901 tcgctgttcc ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg
    12961 taacatatgc tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat
    13021 atgctacccg tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg
    13081 agggtccgct tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt
    13141 agtcttcctg ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca
    13201 agagttacac ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg
    13261 atgaaagcca ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt
    13321 cagagaaccc ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg
    13381 caagttgtac caaccaactg aagggattac at
//
