LOCUS       sequence-194754-       13229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-Kif26b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13229)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 13229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pEF5-FRT-V5-DEST-GFP-Kif26b
FEATURES             Location/Qualifiers
     source          1..13229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        353..1531
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          584..1522
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1479..1499
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1548..1566
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1645..1669
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1697..1699,1700..1702,1703..2413)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1697..1699,1700..1702,1703..2413)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1742..1763)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2003..2022)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2350..2371
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8802..8936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9026..9050)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9103..9144
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(9182..9199)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9188..9412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9545..9564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9695..9742
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9750..10772
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9750..10772
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10902..11023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11072..11088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11072..11088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11085..11107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11096..11112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11120..11126,11127..11144,11145..11150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11165..11186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11303..11320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11474..12062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11554..11573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12233..13024,13025..13093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12856..12875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13094..13198)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgcctcgt gaggctccgg
      361 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      421 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      481 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      541 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      601 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      661 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      721 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      781 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      841 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
      901 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
      961 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1021 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1081 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1141 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1201 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1261 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1321 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1381 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1441 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1501 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1561 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
     1621 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac
     1681 caattcagtc gacaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1741 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1801 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1861 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1921 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1981 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2041 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2101 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2161 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2221 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2281 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2341 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2401 cgagctgtac aagggccggc ctaccatgaa ttcggtagcc ggaaataaag agaggcttgc
     2461 ggtctccacc aggggcaaga aatacggggt gaacgaaatg tgctcgccca ccaagccctc
     2521 agcgccctgc tctcctgaga gctggtaccg caaggcttac gaggagtcgc gcgccggcag
     2581 ccggcccacc ccggagggag caggctctgc gctgggctca tcgggcaccc cgtccccggg
     2641 ctcgggcacc tcgtccccga gctccttcac cggctctccg ggacctgcat ctccgggcat
     2701 tggtaccagc tcgcccggct ctctgggcgg ctcacccggc ttcggcaccg gctcccctgg
     2761 ctcgggcagc ggcggcggct cctcccccgg ctccgaccgc ggcgtctggt gcgagaactg
     2821 taacgcccgc ttggtggagc tgaagaggca ggctctcaag ctgctgctgc cgggacccct
     2881 cccgggaaag gaccctgctt tctctgctgt gattcacgac aaactccagg ttcccaatac
     2941 tatcaggaag tcgtggaatg accgagacaa ccgctgtgac atttgtgcca cacacctgaa
     3001 ccagctaaag caggaagcca tccaaatggt gctgaccttg gagcaggcag ctggcagcga
     3061 gcactatgac acctctctgg gctcaccccc tccgatctcc agcattccca cactggtggg
     3121 gtctcgccac atgggtgggc tccagcagcc cagggagtgg gcctttgtgc ctgcccccta
     3181 tgccacttct acctacacag gccttgtcaa caaacacagc ggcaaaccca acagccttgg
     3241 ggtcagcaac ggagcggaaa agaagagtgg ctccccaacc caccaggcca aggtcagcct
     3301 ccagatggcc accagtccca gcaatggaaa catcctcaac tcggtggcca ttcaggctca
     3361 tcaatatcta gacggcacct ggtccctttc aagaaccaat ggggttaccc tgtaccccta
     3421 ccagatctcc cagctgatga cagagaccgg ccgcgaggga ctgacagagt cagcgctcaa
     3481 ccgctacaat gctgacaagc ctgcagccag cagtgtccca gccccacagg gttcctgtgt
     3541 ggccagtgag acttccacag ggacctcagt ggccgcttcc ttcttcgcac gagctgccca
     3601 gaagttaaat ctgtcatcta agaagaagaa acaccggcct tcgactccat ctgttgcgga
     3661 ggctcccctc tttgcgacca gcttcagtgg gatcctgcag acctctcctc ccccagcccc
     3721 accctgtctc ctgagggctg tcaacaaggt gaaggacact cctggcatgg gcaaggtgaa
     3781 agtcatgctg cgtatctgtt ccacatcagc tcgagatacc tcagaatcca gctctttcct
     3841 gaaggtggac ccacggaaga agcagatcac cttgtatgat cccctgactt gcggaggtca
     3901 aaatgccttc cagaaaagaa gcagccaggt tcctccgaag atgtttgcct tcgatgctgt
     3961 tttcccacaa gatgcttctc aggctgaagt ttgtgctgga actgtggccg aagtcatcca
     4021 gtctgtggtg aacggggcag atggctgtgt gttctgtttt ggccatgcca agctagggaa
     4081 atcctacacc atgatcggaa gggacgactc tatgcagaac ctgggcatta ttccatgtgc
     4141 tatttcttgg ctgttcaagc ttatcaatga gcgcaaagag aagacggggg cccgattctc
     4201 agtccgcatc tcagccgttg aggtgtgggg aaaagaggag aaccttcgag acctgttgtc
     4261 tgaggtggcc acgggcagcc tacaggacgg gcagtcccca ggcgtgtacc tctgtgaaga
     4321 ccccatctgt ggcacacagc tgcagaacca gagtgagctc cgggccccca ccgcagagaa
     4381 agctgccttc ttcctggacg ccgccattgc ctcgaggcga agcaaccaac aggattgtga
     4441 cgaggatgac caccgcaact cccatatgct cttcaccctg cacatctacc agtaccggat
     4501 ggagaagagc gggaagggtg gaatgtctgg aggtcgaagc cgcctgcatc tcatcgacct
     4561 cgggagctgt gtgaaagctc tcagcaaaaa ccgagaagga ggctcggggc tgtgcctctc
     4621 actgtctgct ctgggcaacg tcatcctggc tttggtcaac gggagcaagc acatcccata
     4681 caaggagagc aagctcacca tgcttctgag ggagtctctg gggaatgtga actgtcgcac
     4741 caccatgatc gcacacatct cggccgcagc cagcagctac gcggagaccc tgtccaccat
     4801 ccagatcgcg tcgagagtct taaggatgaa gaagaagaag accaagtaca catcaagctc
     4861 ctctggggga gaaagctcct gtgaggaagg cagaatgcgc aggcccaccc agctgagacc
     4921 cttccatgcc cgggctccgg tggatccaga attccccctt gccccactgt ccagtgatcc
     4981 tgactactcc tccagcagtg agcagtcctg cgacaccgtc atctacatcg ggcccaatgg
     5041 cacagccctg tctgacaagg aactcactga caatgagggg cccccagact ttgtgcccat
     5101 tgtgccagcc ctacagaaga ccagaggtga cagccggccg ggggaggcag cagaggctgc
     5161 agccagcaag tcagaaaggg actgtctgaa gtgcaatacg tttgctgagc tgcaggaaag
     5221 actagattgc atagatggca gcgaagaacc cagtaagttt cccttcgaag agctgcccat
     5281 ccagttcggg ccggagcaag ctggcagatg tgctccttta agccaagcag tggggccaga
     5341 cacaccctct gagtctgaaa aggaagataa cggctcagac aatggccaat tgaccgagag
     5401 ggaaggcaca gagcttccag cctccaaggc acagcggaat cggtcccctg cccctactgt
     5461 gacacgcagc agcagcccca gtccagcctc accacggagc atcccgggca gcagtagcca
     5521 gcacagtacc tcccagctca cacagagccc cagtctccag agcagccggg agagtctgaa
     5581 ctcatgtggc ttcgtggagg gcaagcccag acccatgggc tccccgaggc taggcattgc
     5641 cagtctgtcc aagacctcag agtacaaacc tcccagttct ccctctcaga ggtgcaaagt
     5701 ctacacccag aagggggtcc tgccctcctc tgccccacca ccttccctga gcaaggactc
     5761 tggcatggtc tccagtgaat ccttgcttca gcccgacgtg cgcactcccc cagttggaat
     5821 gagtccccag gttttgaaga aatccatgtc tgccgggagc gaagggtttc ccggaactct
     5881 agttgacggt gaggaccagg aaggccctcc agcggactct aagaaggaga ttctgagcac
     5941 cacaatggtg actgtgcaac agcccctgga gctgaatggt gaggatgagt tggtcttcac
     6001 actggtggag gagctgacca tcagcggggt gctcgacagt ggacgcccta ccagcatcat
     6061 cagcttcaac agtgactgct ccgtgcaggc cctggcctca ggctcccggc ccgtcagcat
     6121 catcagcagc atcagcgagg atctggagtg ctactccagc atggcccccg tctccgaggt
     6181 gagcatcacg cagttcctgc ctctcccaaa gctgggcctg gatgagaagg ctcgggacgc
     6241 gggcagccgg cgttcctcca tcagctcctg gctgagcgag atgagcgcgg gcagtgatgg
     6301 cgagcagtca tgccacagct tcatagccca gacgtgcttt gggcatgggg aagccatggc
     6361 agaaccccca gcctcggagt ttgtcagcag catccagaac acggcagtgg tgtgcagaga
     6421 gaagcccgag gtgggccctg acaacctgct catcttatct gagatggggg aagagtctgg
     6481 caacaaagcc gctcccatca aaggctgcaa aatctcaacg gtgggcaagg ccatggtcac
     6541 catctccaac acagccagcc tgagcagctg cgaagggtac atccccatga agaccaacat
     6601 caccgtttac ccctgcatcg ccatgagccc ccgaaacgtg caagagcctg agtcctccac
     6661 tgccacccca aaggtgagcc ccaaagcatc ccaggcccag gaaagcaagg aacccagtac
     6721 aaggagagag atgaaatttg aggatccttg gcttaaacga gaagaagagg tgaaaaggga
     6781 gaacgcttac tccagcgagg aaggggttaa gtgtgagccg ctcccaagct ccctgaagac
     6841 tgaaggtaag tcagagcagg aactggatgg caggcccagc tcaggcaaca ggctgagcag
     6901 cagcagctca gaggcagccg cctttcaagg gactgataac gttaggagag tcgtggatgg
     6961 ctgtgagatg gccctgcctg gcttggtggc ccaaagtccc ctccatgtca accgaaacct
     7021 aaagtcaagc agcctcccaa gggcatttca gaaagctgac aggcacgagg agctggacag
     7081 cttctaccat tgcctggcag acagcaatgg cttcagtgct gcttcgggca tcccatcctc
     7141 gaagacaacc ctagagagga aagttgcttc acccaagcat tgcgtcctgg caaggcccaa
     7201 agggaccccg cctctgcctc ctgtccgcaa gtctagcttg gaccagaaga acagggccag
     7261 ccctcaacac ggtggtggca gcagcaacac cagcagcccc ctgaaccagc cagctacctt
     7321 cttggcttgc ttccctgatg agtccaacgg caagactaag gatgtcagta gcagcagtaa
     7381 gctcttcagc gccaagctgg aacagctggc cagcagaagc aactctcttg gcaggacaac
     7441 agtcagccac tacgaatgct tgtcgttgga gagggccgaa agcctgtcgt cggtgagctc
     7501 ccgcatgcat gccggcaaag atagtaccat gccccgcact gggaggagcc tgggtcgcag
     7561 cactggcgcc tcaccaccca gctgtggcat cactcagtcc acaggggcct ctcccaaggc
     7621 cagccagtct aagatctctg cagtgagcaa gctcctgctg gccagtccta aatcccgcag
     7681 cagcctgtcc acctccacca ccaaaaccct cagcttctcc accaagtccc tgccacagtc
     7741 tgtgggccag agctccaacc taccgcccag cgggaaacac atgtcctggt ccacccagtc
     7801 gctcagcagg aaccggggct ctgggctggc ttccaaactg ccgctaagag cggtcaacgg
     7861 gcgcatttcg gagctgctcc agggcagtgc gggcccgagg agtgcacagc tgcgggcaga
     7921 ggcggaagag cgcagtgggg ctccctcgga ggacaagccc gcagccgccc acctgctgcc
     7981 ctcgccctac agcaagatca cccctccgag gaagccgcac cgctgcagca gcgggcacgg
     8041 tagtgataac agcagtgtcc tgagtgggga gctaccacct gccatgggga agactgccct
     8101 cttctaccac agcggaggca gcagtggcta cgagagcatg atgagagaca gcgaggccac
     8161 cggcagtgca tcctcagccc aggactccat gagcgagaac agcagctccg tgggagggag
     8221 atgcaggagc ctcaaaaacc aaaagaaaag gtccaattca ggttcccaga gacggaggct
     8281 cattccagca ctgtctctcg ataccccctc acctgtgagg aaaactgcca gcagcacagg
     8341 ggtccgctgg gtggacggcc ccttgaggag cacccagagg agccttgggg aaccattcga
     8401 aattaaagtc tatgagattg atgacgtgga gcgcctgcag cgacgtcgag gagctacaag
     8461 caaggaggtc atgtgcttca atgccaagct gaaaatccta gaacatcggc agcagagaat
     8521 cgctgaagtc cgagccaagt acgagtggct gatgaaggag ctagaagcga ccaagcagta
     8581 cctgatgctg gaccccaaca agtggcttcg tgaatttgac ttggagcagg tcctgcagct
     8641 ggattccctg gagtacctgg aggcactgga gggtgtgaca gagcgcttgg agagccgcgt
     8701 caacttctgc aaggcccatc tcatgatgat cacctgcttt gacatcacct ccaggcgccg
     8761 ataaggcgcg cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat
     8821 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     8881 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     8941 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
     9001 tcgcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt tgatatccag
     9061 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     9121 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttaaac ccgctgatca
     9181 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     9301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     9361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9421 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     9481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9601 gctctaaatc gggggtccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9661 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     9721 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     9781 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     9841 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     9901 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     9961 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
    10021 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
    10081 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
    10141 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
    10201 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
    10261 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
    10321 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
    10381 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
    10441 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
    10501 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
    10561 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
    10621 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
    10681 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
    10741 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
    10801 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10861 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    11041 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    11101 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    11161 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    11221 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    11281 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11881 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    11941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    12001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    12061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    12121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    12181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    12241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    12301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    12361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    12421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    13021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    13081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    13141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    13201 catttccccg aaaagtgcca cctgacgtc
//
