LOCUS       sequence-194781-       10857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd6
FEATURES             Location/Qualifiers
     source          1..10857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3542..3676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3766..3790)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3798..3839
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3890..4478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3943..3963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4361..4372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4550..4783
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4861..4982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5022..5157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5178..5196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5206..5223)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5206..5222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5215..5237)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5364..5819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5451..5470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5661..5682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5845..5949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5950..6018,6019..6810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6168..6187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6981..7569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7470..7489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7723..7740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7857..7878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7893..7898,7899..7916,7917..7923)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7931..7947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7936..7958
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7955..7971
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7955..7971
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7990..8010
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7992..8010
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8038..8264
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8265..8445
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8492..8617
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9110..9343
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9528..9572
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9803..10013
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9825..10021
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9872..10007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10053..10655
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10053..10072)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10549..10569
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10698..10815
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggaa
     1381 aggtccccgt ttctgttggc gtgcattctt ctgcccctcg taagaggaca cagccttttc
     1441 acctgtgagc caatcaccgt tcccagatgt atgaaaatga cttacaacat gacgttcttc
     1501 cctaacctga tgggtcatta tgaccagggg atcgctgctg tggaaatggg gcactttctg
     1561 catcttgcaa atctagaatg ttcaccaaac attgaaatgt tcctttgcca agcttttata
     1621 ccaacctgca cagagcaaat tcatgtagtt ctaccctgtc ggaaattgtg tgagaaaata
     1681 gtttctgatt gcaaaaaact aatggacact tttggcatcc gatggcctga agaacttgaa
     1741 tgtaacagat tgccacactg tgatgacact gttcctgtaa cttctcatcc acacacagag
     1801 ctttctgggc cacagaagaa atcagatcaa gtcccaagag acattggatt ttggtgtcca
     1861 aagcacctta ggacttccgg ggaccaaggc tataggtttc tgggaattga acagtgtgcc
     1921 cctccgtgcc ccaatatgta ttttaaaagt gatgaactag actttgccaa aagtttcata
     1981 ggaatagttt caatattttg tctttgtgca actctgttca cgttccttac atttttaatt
     2041 gacgttagac gattcagata cccagagaga ccaattatct attactctgt ctgctacagc
     2101 attgtctctc tcatgtactt cgtggggttt ttgctgggca atagcacggc ttgtaataag
     2161 gcagacgaga agctggagct cggggacacc gttgtcctag ggtcaaagaa taaggcttgc
     2221 agtgtggtat ttatgtttct gtattttttt acaatggctg gcaccgtgtg gtgggtgatt
     2281 ctcaccatta cgtggttctt agctgccggg agaaaatgga gttgcgaagc tattgaacaa
     2341 aaagcagtgt ggttccatgc cgttgcctgg ggggcgcccg ggttcctgac cgtcatgctg
     2401 ctcgctatga ataaggttga aggagacaac attagcggcg tttgcttcgt tggcctgtat
     2461 gacctggacg cctctcgcta cttcgtcctt ctgcctctgt gcctctgcgt atttgttggg
     2521 ctgtctctcc tcttagccgg catcatctcc ttgaatcatg tccgacaagt catacagcat
     2581 gatggccgga accaagagaa gctaaagaaa ttcatgattc gcatcggagt cttcagtggc
     2641 ctgtatcttg tgcccttagt gacacttctc ggttgctatg tctatgagct agtgaacagg
     2701 atcacctggg agatgacatg gttctctgat cattgtcacc agtaccgcat cccgtgccct
     2761 taccaggcaa atccaaaagc tcgaccagaa ttggctttat ttatgataaa atatctgatg
     2821 acattaattg ttggtatctc tgcggtcttc tgggttggaa gcaaaaagac gtgcacagaa
     2881 tgggccgggt tctttaagcg aaaccgcaag cgagacccca tcagtgagag ccgccgagtg
     2941 ctgcaagagt cctgtgagtt cttcctgaag cacaactcta aagtgaagca caagaagaag
     3001 catggcgcac cagggcctca taggctgaag gtcatttcca agtccatggg aactagcaca
     3061 ggagcgacca caaatcatgg cacctctgcc atggcaatcg ctgaccatga ttacttaggg
     3121 caagaaactt caacagaagt ccacacctcc ccagaagcat ccgtcaaaga gggacgagca
     3181 gaccgagcaa acactcccag cgccaaagat cgggactgtg gggaatctgc agggcccagt
     3241 tccaagctct ctgggaaccg gaacggcagg gaaagccgag cgggcggcct gaaggagaga
     3301 agcaatggat cagagggggc tccaagtgaa ggaagggtaa gtccaaagag cagcgttcct
     3361 gagactggcc tgatagactg cagcacttca caggccgcca gttctccaga accaaccagc
     3421 ctcaagggct ccacatctct gcctgttcac tcagcttcca gagctaggaa agagcagggt
     3481 gctggcagcc attccgacgc ttgaggcgcg cctctagaac tatagtgagt cgtattacgt
     3541 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3601 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3661 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3721 ggtgtgggag gttttttaat tcgcggccgc actcgagata tctagaccca gctttcttgt
     3781 acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     3841 agtaatgaac tagtaccggt taagtcgaca atcaacgcgt taagtcgaca atcaacctct
     3901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4021 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4081 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4141 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4201 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4261 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4321 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4381 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4441 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     4501 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     4561 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     4621 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4681 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4741 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     4801 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     4861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4981 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5041 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5161 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     5221 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5281 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5341 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5401 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5461 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5521 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5581 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5641 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5701 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5761 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     5821 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5881 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5941 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6001 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6061 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6121 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6181 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6241 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6301 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6361 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6421 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6481 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6541 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6601 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6661 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6721 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6781 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7621 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7681 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7741 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7801 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7861 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7921 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7981 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     8041 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8101 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8161 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     8221 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     8281 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8341 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8401 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8461 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8521 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8581 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8641 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8701 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8761 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8821 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8881 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8941 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9001 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9061 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     9121 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     9181 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     9241 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9301 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     9361 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     9421 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9481 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9541 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9601 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     9661 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     9721 cccacctccc aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg
     9781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc
     9841 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9901 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9961 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    10021 aaagctttct agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
    10081 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
    10141 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
    10201 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
    10261 gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
    10321 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
    10381 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
    10441 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
    10501 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
    10561 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
    10621 cctggtgcat gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg
    10681 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
    10741 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
    10801 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccgg
//
