LOCUS       sequence-194785-        9912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9912)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..9912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     RBS             2961..2969
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6640..6761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6677..6696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6731..6750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6768..7223)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6905..6926)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7117..7136
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7250..7354
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7356..7713
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7356..7547
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7379..7399)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7564..7699
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7748..8542
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7748..8542
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7802..7821)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8412..8431
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8730..8749)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8774..8821
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9150..9738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9639..9658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tccggaagtg ctgggagcgc aagcggaagc ggaggctccg gtgaggaaac agctatatgg
     1321 gaacaacata cagtgacgct tcacagggct cctggatttg gatttggaat tgcaatatct
     1381 ggtggacgag ataatcctca ttttcagagt ggggaaacgt caatagtgat ttcagatgtg
     1441 ctgaaaggag gaccagctga aggacagcta caggaaaatg accgagttgc aatggttaac
     1501 ggagtttcaa tggataatgt tgaacatgct tttgctgttc agcaactaag gaaaagtggg
     1561 aaaaatgcaa aaattacaat tagaaggaag aagaaagttc aaataccagt aagtcgtcct
     1621 gatcctgaac cagtatctga taatgaagaa gatagttatg atgaggaaat acatgatcca
     1681 agaagtggcc ggagtggtgt ggttaacaga aggagtgaga agatttggcc gagggataga
     1741 agtgcaagta gagagaggag cttgtccccg cggtcagaca ggcggtcagt ggcttccagc
     1801 cagcctgcta aacctactaa agtcacactg gtgaaatccc ggaaaaatga agaatatggt
     1861 cttcgattgg caagccatat atttgttaag gaaatttcac aagatagttt ggcagcaaga
     1921 gatggcaata ttcaagaagg tgatgttgta ttgaagataa atggtactgt gacagaaaat
     1981 atgtcattga cagatgcaaa gacattgata gaaaggtcta aaggcaaatt aaaaatggta
     2041 gttcaaagag atgaacgggc tacgctattg aatgtccctg atctttctga cagcatccac
     2101 tctgctaatg cctctgagag agacgacatt tcagaaattc agtcactggc atcagatcat
     2161 tctggtcgat cacacgatag gcctccccgc cgcagccggt cacgatctcc tgaccagcgg
     2221 tcagagcctt ctgatcattc caggcactcg ccgcagcagc caagcaatgg cagtctgcgg
     2281 agtagagatg aagagagaat ttctaaacct ggggctgtct caactcctgt aaagcatgct
     2341 gatgatcaca cacctaaaac agtggaagaa gttacagttg aaagaaatga gaaacaaaca
     2401 ccttctcttc cagaaccaaa gcctgtgtat gcccaagttg gcaaccagat gtggatttac
     2461 ctgtcagtcc atctgatggt gtcctaccta attcaactca tgaagatggg atttcttcgg
     2521 cccagcatga aattggtaaa attcagaaaa ggagatagtg tgggtttgcg gctggctggt
     2581 ggaaatgatg ttggaatatt tgtagctggc gttctagaag atagccctgc agccaaggaa
     2641 ggcttagagg aaggtgatca aattctcagg gtaaacaacg tagattttac aaatatcata
     2701 agagaagaag ccgtcctttt cctgcttgac ctccctaaag gagaagaagt gaccatattg
     2761 gctcagaaga agaaggatgt ttatcgtcgc attgtagaat cagatgtagg agattctttc
     2821 tatattagaa cccattttga atatgaaaag gaatctccct atggacttag ttttaacaaa
     2881 ggagaggtgt tccgtgctgt ggataccttg tacaatggaa aactgggctc ttggcttgct
     2941 attcgaattg gtaaaaatca taaggaggta gaacgaggca tcatccctaa taagaacaga
     3001 gctgagcagc tcgccagtgt acagtataca cttccaaaaa cagcaggcgg agaccgtgct
     3061 gacttctgga gattcagagg tcttcgcagc tccaagagaa atcttcgaaa aagcagagag
     3121 gatttgtccg ctcagcctgt tcaaacaaag tttccagctt atgaaagagt ggttcttcga
     3181 gaagctggat ttctgaggcc tgtaaccatt tttggaccaa tagctgatgt tgccagagaa
     3241 aagctggcaa gagaagaacc agatatttat caaattgcaa agagtgaacc acgagacgct
     3301 ggaactgacc aacgtagctc tggctatatt cgcctgcata caataaagca aatcatagat
     3361 caagacaaac atgctttatt agatgtaaca ccaaatgcag ttgatcgtct taactatgcc
     3421 cagtggtatc caattgttgt atttcttaac cctgattcta agcaaggagt aaaaacaatg
     3481 agaatgaggt tatgtccaga atctcggaaa agtgccagga agttatacga gcgatctcat
     3541 aaacttgcta aaaataatca ccatcttttt acaactacaa ttaacttaaa ttcaatgaat
     3601 gatggttggt atggtgcgct gaaagaagca gttcaacaac agcaaaacca gctggtatgg
     3661 gtttccgagg gaaaggcgga tggtgctaca agtgatgacc ttgatttgca tgatgatcgt
     3721 ctgtcctacc tgtcagctcc aggtagtgaa tactcaatgt atagcacgga cagtagacac
     3781 acttctgact atgaagacac agacacagaa ggcggggcct acactgatca agaactagat
     3841 gaaactctta atgatgaggt tgggactcca ccggagtctg ccattacacg gtcctctgag
     3901 cctgtaagag aggactcctc tggaatgcat catgaaaacc aaacatatcc tccttactca
     3961 ccacaagcgc agccacaacc aattcataga atagactccc ctggatttaa gccagcctct
     4021 caacagaaag cagaagcttc atctccagtc ccttaccttt cgcctgaaac aaacccagca
     4081 tcatcaacct ctgctgttaa tcataatgta aatttaacta atgtcagact ggaggagccc
     4141 accccagctc cttccacctc ttactcacca caagctgatt ctttaagaac accaagtact
     4201 gaggcagctc acataatgct aagagatcaa gaaccatcat tgtcgtcgca tgtagatcca
     4261 acaaaggtgt atagaaagga cccatatccc gaggaaatga tgaggcagaa ccatgttttg
     4321 aaacagccag ccgttagtca cccagggcac aggccagaca aagagcctaa tctgacctat
     4381 gaaccccaac tcccatacgt agagaaacaa gccagcagag acctggagca gcccacatac
     4441 agatacgagt cctcaagcta tacggaccag ttttctcgaa actatgaaca tcgtctgcga
     4501 tacgaagatc gcgtccccat gtatgaagaa cagtggtcat attatgatga caaacagccc
     4561 tacccatctc ggccaccttt tgataatcag cactctcaag accttgactc cagacagcat
     4621 cccgaagagt cctcagaacg agggtacttt ccacgttttg aagagccagc ccctctgtct
     4681 tacgacagca gaccacgtta cgaacaggca cctagagcat ccgccctgcg gcacgaagag
     4741 cagccagctc ctgggtatga cacacatggt agactcagac cggaagccca gccccaccct
     4801 tcagcagggc ccaagcctgc agagtccaag cagtattttg agcaatattc acgcagttac
     4861 gagcaagtac caccccaagg atttacctct agagcaggtc attttgagcc tctccatggt
     4921 gctgcagctg tccctccgct gataccttca tctcagcata agccagaagc tctgccttca
     4981 aacaccaaac cactgcctcc acccccaact caaaccgaag aagaggaaga tccagcaatg
     5041 aagccacagt ctgtactcac cagagttaag atgtttgaaa acaaaaggtc tgcatcctta
     5101 gagaccaaga aggatgtaaa tgacactggc agttttaagc ctccagaagt agcatctaaa
     5161 ccttcaggtg ctcccatcat tggtcccaaa cccacttctc agaatcaatt cagtgaacat
     5221 gacaaaactc tgtacaggat tccagaacct caaaaacctc aactgaagcc acctgaagat
     5281 attgttcggt ccaatcatta tgaccctgaa gaagatgaag aatattatcg aaaacagctg
     5341 tcatactttg accgaagaag ttttgagaat aagcctcctg cacacattgc tgccagccat
     5401 ctctccgagc ctgcaaagcc agctcattct cagaatcaat caaatttttc tagttattct
     5461 tcaaagggaa agcctcctga agctgatggt gtggatagat catttggcga gaaacgctat
     5521 gaacccatcc aggccactcc ccctcctcct ccattgccct cgcagtatgc ccagccatct
     5581 cagcctgtca ccagcgcgtc tctccacata cattctaagg gagcacatgg tgaaggtaat
     5641 tcagtgtcat tggattttca gaattcctta gtgtccaaac cagacccacc tccatctcag
     5701 aataagccag caactttcag accaccaaac cgagaagata ctgctcaggc agctttctat
     5761 ccccagaaaa gttttccaga taaagcccca gttaatggaa ctgaacagac tcagaaaaca
     5821 gtcactccag catacaatcg attcacacca aaaccatata caagttctgc ccgaccattt
     5881 gaacgcaagt ttgaaagtcc taaattcaat cacaatcttc tgccaagtga aactgcacat
     5941 aaacctgact tgtcttcaaa aactcccact tctccaaaaa ctcttgtgaa atcgcacagt
     6001 ttggcacagc ctcctgagtt tgacagtgga gttgaaactt tctctatcca tgcagagaag
     6061 cctaaatatc aaataaataa tatcagcaca gtgcctaaag ctattcctgt gagtccttca
     6121 gctgtggaag aggatgaaga tgaagatggt catactgtgg tggccacagc ccgaggcata
     6181 tttaacagca atgggggcgt gctgagttcc atagaaactg gtgttagtat aattatccct
     6241 caaggagcca ttcccgaagg agttgagcag gaaatctatt tcaaggtctg ccgggacaac
     6301 agcatccttc cacctttaga taaagagaaa ggtgaaacac tgctgagtcc tttggtgatg
     6361 tgtggtcccc atggcctcaa gttcctgaag cctgtggagc tgcgcttacc acactgtgat
     6421 cctaaaacct ggcaaaacaa gtgtcttccc ggagatccaa attatctcgt tggagcaaac
     6481 tgtgtttctg tccttattga ccacttttaa ggatccaccg gatctagata actgatcata
     6541 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     6601 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     6661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6721 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     6781 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6841 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6901 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6961 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7021 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7081 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7141 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7201 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7321 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     7381 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7441 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7501 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7561 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7621 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7681 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     7741 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7801 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7861 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7921 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7981 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     8041 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     8101 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     8161 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     8221 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     8281 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     8341 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     8401 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     8461 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     8521 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     8581 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8641 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8701 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     8761 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     8821 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     8881 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     8941 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     9001 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9061 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9781 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9841 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     9901 tatggagttc cg
//
