LOCUS       sequence-194936-        3608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the fluorescent protein meffCFP for constitutive expression 
            in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, 
            Hughes TE, Drobizhev M
  TITLE     Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than 
            Enhanced Green Fluorescent Protein under Two-Photon Excitation.
  JOURNAL   J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 
            10.1021/acs.jpclett.7b00960. Epub 2017 May 25.
  PUBMED    28530831
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpclett.7b00960"; journalName: "J Phys Chem Lett"; 
            date: "2017-06-15- 15"; volume: "8"; issue: "12"; pages: "2548-2554"
COMMENT     Sequence Label: pRSM12
FEATURES             Location/Qualifiers
     source          1..3608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..82)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      78..125
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      222..677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(309..328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     519..540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        703..807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(808..876,877..1668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1026..1045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1839..2427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2328..2347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2581..2598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2683..2702
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2683..2701
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2785..2807
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2827..2844
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2848..2880
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(2884..2892,2893..2907)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ggaattcgaa gcttgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
       61 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      121 ttttgctgaa aggaggaact atatccggat ctggcgtaat agcgaagagg ccagcaccga
      181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      661 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      721 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      781 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      841 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      901 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      961 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1021 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1081 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1141 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1201 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1261 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1321 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1381 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1441 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1501 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1561 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1621 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1681 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1741 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1921 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1981 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2041 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2101 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2161 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2221 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2281 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2341 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2401 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2461 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2521 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2581 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2641 cgttggccga ttcattaatg caggatctcg atcccgcgaa attaatacga ctcactatag
     2701 ggagaccaca acggtttccc tctagaatca ccgagtttat tcttgacacc tgatgcgatg
     2761 aatgatataa taggaaagta ctgttttgtt taactttaag aaggagatat acatatgcgg
     2821 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
     2881 cgggatctgt acgacgatga cgataaggat ccggcactta gcaagcaatc cctgcccagc
     2941 gatatgaagc tgatctatca catggatggg aacgttaatg ggcactcttt cgtgatcaaa
     3001 ggggagggag agggcaagcc atacgaaggc acacacacta ttaagctgca ggtcgtggaa
     3061 ggtagtcctc tgcctttctc cgccgatatc ctgagcaccg tcttccagta cggcaaccgg
     3121 tgctttacga agtacccacc caacatcgtg gactacttta agaatagctg cagcgggggc
     3181 ggatacaagt ttggccggag ctttctgtat gaggacggtg ccgtgtgcac cgcgtcagga
     3241 gatataacac tgtctgccga caaaaagtca ttcgaacaca aatctaaatt cctgggtgtg
     3301 aactttcccg cagacggccc cgtgatgaag aaagaaacta ccaattggga gccatcctgt
     3361 gagaaaatga ccccgaacgg catgaccttg attggagacg taaccggatt cctcctcaaa
     3421 gaggacggca agcggtataa gtgtcagttt cacacattcc atgatgccaa ggacaagagc
     3481 aaaaagatgc ctatgcccga cttccacttt gtgcagcata agatcgagag aaaagatctg
     3541 cccgggagta tgcagacctg gcggctgacc gagcatgctg ctgcctgtaa aacatgcttc
     3601 actgaata
//
