LOCUS       sequence-195155-       11636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of IKZF3 with Q147H mutation and FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1000
                     /label=chicken beta-actin promoter
     intron          1001..2009
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1933..1955
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2017..2036
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2125..2149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3677..3701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3721..3744
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3779..4328
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3923..3940)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4150..4169
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4331..4333,4334..4336,4337..5050)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4331..4333,4334..4336,4337..5047)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4376..4397)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4637..4656)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4984..5005
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5127..5715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5180..5200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5598..5609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5718..5734)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5719..5735)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5924..6157
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6229..6350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6266..6285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6320..6339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6390..6525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6545..6564)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6546..6564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6587..6945)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6627..6648)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6839..6858
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6972..7076
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7078..7435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7078..7269
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7101..7121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7286..7421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7470..8264
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7470..8264
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7524..7543)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8134..8153
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8452..8471)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8496..8543
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8872..9460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9361..9380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9635..9938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9940..10138
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10093..10113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10156..10336
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10383..10508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11005..11238
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11423..11467
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc
     1021 gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     1081 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     1141 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg
     1201 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     1261 ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1321 tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1381 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1441 tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac
     1501 ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc
     1561 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1621 tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1681 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1741 ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg
     1801 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1861 ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt cgggggggac
     1921 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1981 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     2041 ctcatcattt tggcaaagaa ttcccctagc cgcggaatct agactgccgg atcgaattgt
     2101 cgacgaattc ggatctggcc aatcacaagt ttgtacaaaa aagttggcat ggaagatata
     2161 caaacaaatg cggaactgaa aagcactcag gagcagtctg tgcccgcaga aagtgcagcg
     2221 gttttgaatg actacagttt aaccaaatct catgaaatgg aaaatgtgga cagtggagaa
     2281 ggcccagcca atgaagatga agacatagga gatgattcaa tgaaagtgaa agatgaatac
     2341 agtgaaagag atgagaatgt tttaaagtca gaacccatgg gaaatgcaga agagcctgaa
     2401 atcccttaca gctattcaag agaatataat gaatatgaaa acattaagtt ggagagacat
     2461 gttgtctcat tcgatagtag caggccaacc agtggaaaga tgaactgcga tgtgtgtgga
     2521 ttatcctgca tcagcttcaa tgtcttaatg gttcataagc gaagccatac tggtgaacgc
     2581 ccattccact gtaatcagtg tggggcatct tttactcaga aaggtaacct cctccgccac
     2641 attaaactgc acacagggga aaaacctttt aagtgtcacc tctgcaacta tgcatgccaa
     2701 agaagagatg cgctcacggg gcatcttagg acacattctg tggagaaacc ctacaaatgt
     2761 gagttttgtg gaaggagtta caagcagaga agttcccttg aggagcacaa ggagcgctgc
     2821 cgtacatttc ttcagagcac tgacccaggg gacactgcaa gtgcggaggc aagacacatc
     2881 aaagcagaga tgggaagtga aagagctctc gtactggaca gattagcaag caatgtggca
     2941 aaacgaaaaa gctcaatgcc tcagaaattc attggtgaga agcgccactg ctttgatgtc
     3001 aactataatt caagttacat gtatgagaaa gagagtgagc tcatacagac ccgcatgatg
     3061 gaccaagcca tcaataacgc catcagctat cttggcgccg aagccctgcg ccccttggtc
     3121 cagacaccgc ctgctcccac ctcggagatg gttccagtta tcagcagcat gtatcccata
     3181 gccctcaccc gggctgagat gtcaaacggt gcccctcaag agctggaaaa gaaaagcatc
     3241 caccttccag agaagagcgt gccttctgag agaggcctct ctcccaacaa tagtggccac
     3301 gactccacgg acactgacag caaccatgaa gaacgccaga atcacatcta tcagcaaaat
     3361 cacatggtcc tgtctcgggc ccgcaatggg atgccacttc tgaaggaggt tccccgctct
     3421 tacgaactcc tcaagccccc gcccatctgc ccaagagact ccgtcaaagt gatcaacaag
     3481 gaaggggagg tgatggatgt gtatcggtgt gaccactgcc gcgtcctctt cctggactat
     3541 gtgatgttca cgattcacat gggctgccac ggcttccgtg accctttcga gtgtaacatg
     3601 tgtggatatc gaagccatga tcggtatgag ttctcgtctc acatagccag aggagaacac
     3661 agagccctgc tgaagtgccc aactttcttg tacaaagtgg tgggaggagg tggatctggc
     3721 gactacaagg acgacgatga caagtgactc gagcgggatc aattccgccc cccccctaac
     3781 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3841 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3901 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3961 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4021 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4081 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4141 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4201 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4261 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4321 cgataatacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4381 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4441 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4501 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     4561 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4621 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4681 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4741 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     4801 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     4861 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4921 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4981 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5041 gtacaagtaa agcggccgcg actctagagt cgacctgcag gcatgcaagc ttcaggtagc
     5101 cggctaacgt tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5161 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5221 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5281 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5341 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5401 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5461 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5521 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5581 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5641 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5701 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
     5761 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5821 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5881 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5941 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6121 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6181 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6361 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6541 ttcgccctat agtgagtcgt attagcatgt gagcaaaagg ccagcataaa tcaaaagaat
     6601 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6661 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6721 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6781 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6841 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6901 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     6961 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7021 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     7081 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7201 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7261 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7321 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7381 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     7441 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7501 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7561 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7621 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     7681 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7741 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7801 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7861 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7921 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7981 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     8041 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8101 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8161 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8221 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8281 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8341 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8401 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     8461 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     8521 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     8581 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     8641 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     8701 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     8761 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8821 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8881 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8941 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9001 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9061 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9121 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9181 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9241 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9301 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9361 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9421 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9481 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9541 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     9601 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     9661 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     9721 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     9781 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     9841 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     9901 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     9961 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
    10021 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
    10081 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
    10141 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    10201 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    10261 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10321 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    10381 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    10441 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    10501 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    10561 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    10621 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    10681 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    10741 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    10801 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
    10861 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    10921 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    10981 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    11041 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    11101 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    11161 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    11221 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    11281 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    11341 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    11401 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    11461 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    11521 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    11581 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggg
//
