LOCUS       sequence-195167-       11655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SOX10 gRNA used for targeted demethylation is inserted into the WPXL
            lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V,
            Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, 
            Huangfu D
  TITLE     TET proteins safeguard bivalent promoters from de novo methylation 
            in human embryonic stem cells.
  JOURNAL   Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. 
            Epub 2017 Dec 4.
  PUBMED    29203910
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-017-0002-y"; journalName: "Nat Genet"; date: 
            "2018-01"; volume: "50"; issue: "1"; pages: "83-95"
COMMENT     Sequence Label: WPXL SOX10 gRNA
FEATURES             Location/Qualifiers
     source          1..11655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3352..3399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3418..4443
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3418..4443
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4557..4678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4707..4824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4914..4938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4955..5203
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4955..4975
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5126..5145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5225..5300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5418..5442)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5486..6074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5539..5559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5957..5968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6218..6251)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6271..6451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6476..6494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6477..6494)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6784..6803)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7017..7036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7136..7158
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7196..7214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7282..7386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7387..7455,7456..8247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7605..7624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8418..9006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8907..8926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9160..9177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9247..9267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9252..9581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9432..9567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10715..10780
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10910..10930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11355..11489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tgttgacaat
     3361 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     3421 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     3481 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     3541 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     3601 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     3661 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     3721 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     3781 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     3841 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     3901 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     3961 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     4021 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     4081 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     4141 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     4201 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     4261 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     4321 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     4381 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     4441 tagtctagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4501 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatt
     4681 aattaatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     4741 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4801 caaattacaa aaattcaaaa ttttatcgat cacgagacta gcctcgaggt ttaaactacg
     4861 ggatccaggc ctaagcttac gcgtcccagt gtggtggaat tctgcagata tcaacaagtt
     4921 tgtacaaaaa agcaggctcc gcggtggcgg ccgcgagggc ctatttccca tgattccttc
     4981 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     5041 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     5101 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     5161 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcacagt aagagagact
     5221 tctcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     5281 aagtggcacc gagtcggtgc ttttttgttt tagagctaga aatagcaagt taaaataagg
     5341 ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa tggctctaga ggtacgaatt
     5401 caagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     5461 tcgacgataa aactagtcat atgataatca acctctggat tacaaaattt gtgaaagatt
     5521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6001 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6061 ggccgcctcc ccgcatcggt acgtatggcc aggtaccttt aagaccaatg acttacaagg
     6121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6181 cccaacgaag acaagatggg atcaattcac catgggaata acttcgtata gcatacatta
     6241 tacgaagtta tgctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6421 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     6481 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     6541 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     6601 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     6661 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     6721 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     6781 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     6841 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     6901 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     6961 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     7021 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7081 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7141 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     7201 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7261 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7321 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7381 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7441 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7501 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7561 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7621 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7681 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7741 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7801 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7861 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7921 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7981 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8041 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8101 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8161 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8221 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8281 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8341 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8461 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8521 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8581 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8641 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8701 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8761 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8821 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8881 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8941 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9001 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9061 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9181 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9241 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9301 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     9601 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     9661 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     9721 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     9781 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     9841 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     9901 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     9961 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
    10021 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
    10081 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
    10141 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
    10201 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
    10261 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
    10321 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
    10381 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
    10441 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
    10501 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
    10561 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
    10621 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
    10681 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
    10741 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
    10801 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
    10861 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
    10921 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
    10981 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
    11041 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
    11101 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
    11161 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
    11221 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
    11281 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    11341 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11461 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    11521 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    11581 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    11641 ctacaaactt agtag
//
