LOCUS       sequence-195211-        4191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-B5JU35
FEATURES             Location/Qualifiers
     source          1..4191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaccaaga actatatcag cccaattgcc atcgacctgg gggcgaaatt cacaggcgtc
       61 gctctctatc aatatctgga aggcgcagat tgcacccagg aagtcgccaa gggcctcctc
      121 gtggatgacc ggggtaacgt cacctggtcc caagagggaa gacgcggaaa gcggcaccag
      181 gtccgaggat ataagagacg gaagatggcc aagcgcctgc tgtggctcat attggacagc
      241 gaatacggca taaagagaga ggaagtgacc gagcccctcc tcaaatttat caatggcctc
      301 ttgaaccggc gcgggtacac ttatatatcc gaggaagtgg acgaagaatc tatgaatgtc
      361 tcccccctcc ccttctccga gatgatgcca gattacttta attcttctgc ccccttgctg
      421 gagcagcttg ccaaactcct gagcgacaag aacaaacttg tccggtttcg ggccgaaggc
      481 aagatcccta gtaataagaa cgagttcaag aaactgctcg acaccgccct ggacgggaag
      541 tacaaagatg agaaaaaaga gctgtccgag gcctggggca acatactgat cgcttccgaa
      601 aatgtcctca aatctactgt cgatggccac aagtctagaa gcgagtatct ggcaaacatc
      661 aaagaggaca tcaagagcaa tgaagaactt gagaagcaga taagtagtaa ggagatcgac
      721 ggattttata atctggtcgg acacctgtca aacttccaac ttaggttgct ccggaagtat
      781 ttcaatgacc ccaatatgag tggcgtgtct tattgggatg agaagcggct ggaaaaatac
      841 ttctaccagt gggtacaggg atggcacact aaaggaggta ccgacgaggc tgaaaagaaa
      901 aatataatcc tgaagaccaa aggggctcct ctgctgaaaa ccctcaagag tctgtctgcc
      961 gacctgacta taccccccta cgaggatcaa aataaccggc gaccgccgaa atgccagagc
     1021 gtgctgctga gtgatgagaa actcaccatg cattacccca agtggaaaga atgggtggga
     1081 cagttggtga agcaaaatga caatgcctac ctgaatgaga acgtcacctt ggccaacgcc
     1141 ctgcacagga tcgtcgagag atctcgaagt atagacccat atcagctgag actcttgatc
     1201 tccattaccg acgctgagaa gcgcaatgac cttgctggat acaagaggct caagctcagt
     1261 ttgggtagcg aggtggacga gtttctgctt ttggtgaaaa atatcgtgga tgagacaaag
     1321 gaggcacgcg agggtctgtg gttcgagaca gagaacaaac ttttctttaa atgcggtaag
     1381 actcccccac gcaaagaaaa gctcaagtct actctgctga gcgctgtcct cgggaagaac
     1441 ctctccgacg atgaacagag ctcttttatt gaggagtttt ggaaaagcgg gaccccaaaa
     1501 attgaacgaa ggaacgtgag aggctggtgc cgcttggcct cccaagtgca gaagacctac
     1561 ggggtgtact tgaaggagta cggcctccaa cagctccaca aattggaggc tggcaaaaaa
     1621 ctggatgaca agccactggc tctgctgtat aagaatagtg gcctgattgc aagcaagatc
     1681 ggtgaagcct tgaacatcga acccgatgaa gtctcccgat ttgcatctcc acatagtctg
     1741 gctcagatct ttaatatcat tgagggcgat gtagccggat ttaacaaaac ttgtcgcgca
     1801 tgcacctacg aaaacatttg gagaatgcag gaggaaaagg tagagagcct cctgacgaat
     1861 cagctgctct ctgaaattca cggggaaagg aaagtgccgt tgaaatccgc aatgtgcaca
     1921 agactttccg ccgatagcac ccgacctttt gatggccaga tggcgagcat tattgaacac
     1981 atagcccgca agatagccca acacaaaatt gcacagatca atgacgttcc caaggaattt
     2041 agtatcgata ttcctatcat aatcgaaagc aatcagtttt ccttcactgc tgaactcgag
     2101 gagatcaaac ggggcagagg ctctgcaaaa gctaaaaaag ccaaggagct cggcgagaag
     2161 tctaaagcag gctgggtcag caagactgag aggatcaaga ccagcagcga aggtatctgc
     2221 ccatacactg gcgccccact cgggggatct ggtgaaattg accacattat accccgcagt
     2281 ctcaccggcc ggaccaaaaa gacagtcttc aacagcgagg caaatctcat atattgctca
     2341 tccaaaggga atcacgataa aggaaatcgg gtttatgtga ttgagcagct caatgacaag
     2401 tatttgaaaa agcagttcag tacctcagac gtcaacctga tcaaaaagaa aatcaagacc
     2461 actatccaga ggtttactga agggggcgag aagttgcgca gctttagcga actgtctcgc
     2521 gaagatcaga aggcctttag acatgcgctg ttcgtgcctg aacttaagtc cgaagtgacc
     2581 tccctgttgg cagtgaagaa tattactcgg gtgaacggaa cgcaggcctg gctcgcaaag
     2641 aagatagcca gcctgctggc cgagcatttg gataagcagg ggagagacta tacactgagc
     2701 gcgcatcaga tcgacccctg gagtgtaagt aagcagcgaa aaatgcttgc atctgccgag
     2761 cctatttggg ctaaaaagga cccgcaacca gcggcctctc atgtggtgga cgctgtgtgt
     2821 acattcctcg aggccttgga acagccgcac accgcatccc ggcttaaaac tattagctca
     2881 accagtttcg agaagacagg ctggcgcagc gcactgatcc cagatctgat caaagttgac
     2941 gcactggacc ggcggcctaa gtacagacgc tataatatag gctcaacttc tctttttaaa
     3001 gacgggatct atgccgagcg gttcctcccc atcctcattg atgaaaacgg cctgatggcc
     3061 gggtatgaca tcgataatag tctgaaagcc aagggcgccg acgtggtctt tgagtccctg
     3121 agtcctttcc ttctgttcaa aggagaggag gtgggcgctc aaagcctctc agactggcag
     3181 gagcgcatag atggccgata cctctacatg tctatcgaca aagtaaaggc cttcgattac
     3241 ttgcaagaaa aagtaggcga gaaagacatc gctgccgagc tgctgaacag catccatttc
     3301 acccagcgga agaccgagct gagagccaag ttcagcgacg actcaggcaa gaagatgaaa
     3361 accctggacg caatcaggaa gtccctcaag cttaccgtga cagtgaacga gattgggaaa
     3421 cgcaaggaga agtgcggttt cagcggaaca atagggatcc cagccaagtc cgcttgggag
     3481 aatctgctgg atgagccctt gcttgaaacc tattggggca caaaaatgcc tcctcaagag
     3541 atctgggaga aagtatatag gaagcacttc cctaggaaca taccaaacca ggcacatagg
     3601 aaggtgcgaa aggacttttc cctgccagtc gtggattctg tctctggtgg gttcagggtc
     3661 aaacgcaaaa cccctaacgg ctacaattac cagttgctgg caattgatgg gtactcagcg
     3721 gttggattca agaaggaggg tgataatgtc gactttaaga gccctgccct ggtcccccag
     3781 atagcggaaa gcaagagcgt gacccccatc agtagcgagc tcgttcatct cgacaagaat
     3841 gaaattgtgt acttcgacga gtggcgcaag atagatataa gcgactctga tcttaagcag
     3901 ttcgtgtcca gccttgaact ggcacccggc tctcaaaacc ggttctacat ccgattcact
     3961 gtggacgagg accagttcga aaggcacttt aaatctgccc tgcgggtcaa cggcattcag
     4021 gacctggaca ctgtgaataa gaccttcgat tggaaccgcg aaatcccctc ccttttgatt
     4081 cctcctaggt ccaacctgtt tctcctggaa accgggcaga agatcacttt cgagtatatt
     4141 gcaaatggag ctaacgccga ggtgaagaag gcctactctc tgcgccgggc a
//
