LOCUS       sequence-195235-        4260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E3ELL7
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagcagaa aaaactatgt cgacgactac gcaataagcc tggacatcgg aaacgcttcc
       61 gtggggtgga gcgcctttac ccctaactat cgactggtgc gcgccaaggg tcacgagctc
      121 atcggcgtta gactctttga tccagccgat actgcagaaa gtagaagaat ggcgaggaca
      181 accagacgca gatactctcg aaggcgctgg cgactgcggt tgttggatgc actgttcgat
      241 caggctctta gtgaaattga tccatccttc cttgctagga gaaaatactc ctgggtgcat
      301 cctgatgatg aaaataacgc cgattgttgg tatggctctg tcctgttcga ttctaacgaa
      361 caggataaga gattttatga gaaatatccc acaatttacc accttaggaa ggcacttatg
      421 gaggacgaca gccaacatga tatacgggag atttacctgg ccatccacca catggtcaaa
      481 tatcggggca actttctggt agaaggcaca ctggagtcct ccaacgcttt taaggaggac
      541 gagctgctga agctgcttgg aaggatcacc cgatatgaga tgagcgaagg cgagcaaaat
      601 tctgatatcg agcaggatga cgagaacaaa ttggtggcac ccgcaaacgg ccaattggct
      661 gacgccctct gtgcaaccag aggcagccgc tcaatgcgcg tggacaacgc gcttgaggca
      721 ctgtctgcgg tgaacgatct cagccgcgag caaagggcaa tcgttaaagc catctttgct
      781 ggcctcgagg gtaacaagct ggacctggca aagattttcg tctcaaagga gtttagttct
      841 gaaaacaaga agatcctcgg catctacttc aataagtcag actacgagga gaagtgtgtc
      901 cagatcgtcg actccggact gctcgacgac gaggaacgcg aattccttga tcggatgcaa
      961 ggccagtaca acgcaatcgc tttgaagcaa ctgctgggca ggtctacaag tgttagcgac
     1021 agcaagtgcg catcatacga cgcacacaga gcgaactgga atctcatcaa actgcaactc
     1081 agaaccaaag agaacgagaa ggacatcaac gagaactacg gcatccttgt cgggtggaaa
     1141 attgactcag ggcaaagaaa gtccgtgagg ggcgagtccg cttacgaaaa tatgcgcaag
     1201 aaggctaatg tcttttttaa aaagatgatt gagacaagcg acctgtcaga gacagacaaa
     1261 aaccgactga tccatgatat cgaggaggat aagctgttcc cgatacagag agattctgac
     1321 aatggagtca tcccacacca acttcaccaa aacgagttga agcagatcat taagaaacag
     1381 ggaaagtact atccatttct gctggacgca ttcgagaagg atggtaaaca aattaacaaa
     1441 atcgagggcc tcttgacatt cagggtgcca tattttgtgg gacccctcgt tgtcccagaa
     1501 gacctgcaaa aatccgataa tagcgagaac cattggatgg taaggaagaa gaaaggagag
     1561 atcaccccct ggaacttcga cgagatggtc gacaaggatg ctagcgggag aaagtttata
     1621 gagcgattgg ttggcacgga ctcctacctt ctgggagagc ccacactgcc taagaattct
     1681 ctcctgtatc aggagtacga agtgttgaac gagctcaata atgttcgcct gagtgtcagg
     1741 accggcaacc actggaatga taaaaggaga atgagactgg gcagagagga aaagacgctg
     1801 ttgtgtcaga ggctcttcat gaagggacaa acggtgacca agcgcactgc cgagaacctc
     1861 ctgcgcaagg agtacggtcg cacctatgag cttagcggac tgtcagacga aagtaaattt
     1921 acctcaagtc tgagtacata cggcaaaatg tgcagaatct ttggtgaaaa gtacgttaac
     1981 gagcaccggg atttgatgga gaagattgtt gaactgcaga ccgtatttga ggacaaggaa
     2041 accctcctgc accagctgcg ccaactggag gggatatctg aggcagattg tgcactgctg
     2101 gtgaacaccc actatacagg ctggggccgc ctctcaagaa agttgctcac aactaaagct
     2161 ggcgagtgta agatcagcga tgatttcgca ccccggaaac actcaattat tgagattatg
     2221 cgcgccgagg accgcaatct gatggagata atcacagata agcaactggg attcagtgat
     2281 tggatcgagc aggagaatct cggagccgag aacggcagca gccttatgga agttgttgat
     2341 gacctgagag tgtctcccaa agtcaaacgc ggtattattc agagtattcg actgattgat
     2401 gacattagta aggccgttgg gaaacgcccg tctcgaatct tcttggaatt ggctgatgac
     2461 attcagcctt ccggaagaac tatctcacga aaatcccgac tccaagatct ctatcgaaac
     2521 gccaacctgg gtaaagagtt taagggtata gcagatgagc ttaacgcctg tagcgataaa
     2581 gatctgcagg atgatcggct gttcctttat tacacacagc tgggaaaaga tatgtatacc
     2641 ggagaggagc tcgacttgga ccgattgtcc agcgcttacg acatcgatca tattattcca
     2701 caggcagtca ctcaaaacga ctccatcgac aaccgggtgc tcgtagccag agcagagaac
     2761 gcacgaaaga ccgactcctt cacatatatg ccccagattg cagataggat gagaaacttt
     2821 tggcagatcc tcctggacaa tgggctgatc tctcgcgtga aattcgagcg gctcacaaga
     2881 cagaacgaat tctccgaaag ggagaaggag aggttcgtcc agcggtcact ggtcgaaacc
     2941 aggcagatta tgaaaaatgt ggcaacactg atgcgccaga gatacgggaa ttccgcggcc
     3001 gtgattggac tgaatgcgga gctgacgaag gagatgcacc ggtacttggg attcagccac
     3061 aaaaataggg atattaatga ctaccaccac gcccaggatg cgctttgcgt gggaatagcc
     3121 gggcaatttg cggctaatag ggggttcttt gcagacggcg aggtttccga cggtgcccag
     3181 aacagctata atcagtacct tagagattac ctgcgaggct acagagagaa actctccgcg
     3241 gaggatagaa agcaagggag agcctttggg ttcattgtgg gcagcatgcg gagccaagac
     3301 gagcagaaga gggtaaatcc caggacagga gaggtggtct ggagcgaaga agacaaggac
     3361 tacctcagga aagtgatgaa ctatcgaaag atgctggtca ctcagaaagt gggagatgat
     3421 tttggggctc tgtacgacga gacaagatac gcagccaccg atccaaaggg gattaaaggg
     3481 attccttttg acggagctaa acaggacacc tccctgtatg gtggattctc ctctgcgaag
     3541 cccgcctatg ccgtgttgat cgaaagtaaa ggcaagacac gactcgtgaa cgtgacaatg
     3601 caagagtata gcctgttggg cgatagacct tccgatgacg aactgcgcaa ggtgctggcc
     3661 aaaaagaaga gcgaatatgc caaggccaat atacttttgc gccacgttcc caaaatgcag
     3721 ctcatcagat acggcggggg actgatggtg atcaagtcag cgggggaact gaacaacgca
     3781 cagcagctgt ggcttcccta tgaggaatac tgttacttcg atgacctgtc acagggaaag
     3841 ggcagtcttg agaaggatga cctcaaaaag ttgctcgata gtatcctggg ttctgtccag
     3901 tgtctctacc catggcacag atttactgag gaggaactgg ccgatctgca cgttgcattc
     3961 gacaagctgc ccgaagatga gaaaaaaaac gttattaccg gtatagtgtc cgcccttcat
     4021 gcagacgcca agacagcaaa cctttcaatt gtgggaatga ccggcagctg gaggcgaatg
     4081 aacaacaaga gtgggtacac tttcagcgat gaggacgaat tcatctttca atccccttct
     4141 ggattgtttg agaagagagt gaccgtgggt gagcttaagc gaaaagccaa gaaggaggtg
     4201 aactctaagt accgaactaa cgaaaagcgc ttgccaacac tgagtggggc tagccagcct
//
