LOCUS       sequence-195283-        4164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-Q03JI6
FEATURES             Location/Qualifiers
     source          1..4164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaccaagc catactctat tggcttggac attgggacaa attcagtggg ttgggcggtg
       61 actactgaca attacaaggt cccctcaaag aagatgaaag tgctcggaaa cacgagcaaa
      121 aagtacataa agaagaacct cttgggggtg cttctgttcg attcaggaat aaccgctgag
      181 gggcgcaggt tgaaacgcac tgccaggcgg cgctacactc ggaggcggaa ccggattctg
      241 tatctgcagg agatcttcag tacagaaatg gctactctgg atgatgcctt ctttcagcgg
      301 ctcgatgaca gtttcctggt ccctgacgac aaaagggaca gcaagtaccc cattttcggg
      361 aacctcgtcg aggagaaggc gtaccacgac gaatttccca ccatctatca tcttagaaag
      421 tacctggccg atagcacaaa gaaggccgat ctcagacttg tatatctggc cttggctcac
      481 atgattaaat accgcggtca tttcctgatc gaaggcgaat tcaacagtaa gaacaacgat
      541 atccagaaaa acttccagga ttttctggac acttataacg ccatattcga gtcagacctg
      601 tcccttgaaa atagcaaaca actggaggaa atcgtgaaag ataagattag caagctggaa
      661 aaaaaggata gaattctgaa acttttccct ggcgagaaga attccgggat tttttccgaa
      721 tttctgaagc tgatagttgg caatcaggct gacttccgga aatgtttcaa cctggatgag
      781 aaggcgagtc tgcacttcag caaggagtct tacgacgaag acctggaaac actgctcggc
      841 tatatcgggg atgattactc agacgtgttc ctgaaagcaa aaaagcttta tgacgcaatc
      901 ttgttgtcag gcttcctgac tgttactgac aacgagacgg aagcacccct ctctagcgca
      961 atgataaaga ggtataacga gcataaagag gacttggccc ttttgaagga gtacattagg
     1021 aacatcagtc tcaagaccta taacgaggtg ttcaaagacg atactaagaa tggctacgct
     1081 ggttatattg atgggaagac caatcaggag gatttttatg tatatctcaa aaaactcctc
     1141 gcagagttcg agggagctga ttacttcttg gagaagatcg atcgcgagga ctttctcagg
     1201 aagcagagaa ccttcgacaa tggttccatc ccttatcaga tccatcttca ggagatgcgc
     1261 gccatcctgg acaaacaggc caagttctac cctttcttgg caaaaaataa ggaacgcatt
     1321 gagaaaatcc tgacatttag aatcccttat tacgtgggcc ccctggcacg cggaaattct
     1381 gacttcgcct ggagcattcg aaaacggaat gaaaagatca caccttggaa cttcgaggac
     1441 gttatagaca aggagtcctc cgctgaagca ttcatcaatc ggatgacctc cttcgacttg
     1501 tatttgccag aggagaaggt gttgccaaaa cacagtctgc tctacgagac tttcaatgtg
     1561 tacaatgagc tgaccaaagt cagattcatt gccgagtcaa tgagagatta tcagttcctg
     1621 gattctaaac agaaaaaaga tattgtgcgc ctgtatttca aggataaacg gaaagtgacg
     1681 gacaaagaca ttattgagta tctgcacgcc atctacggtt atgacggcat cgaactcaaa
     1741 ggaatcgaga aacagttcaa cagttccctg agtacttatc acgatctcct gaatatcatc
     1801 aacgacaaag aatttcttga cgacagttca aacgaggcta ttattgaaga gatcatccac
     1861 acactcacga tattcgaaga cagagaaatg attaagcaga gactgtcaaa attcgaaaat
     1921 atttttgata agtctgtgct gaagaagctc agtaggaggc attacaccgg gtggggcaaa
     1981 ctttcagcta agctgattaa cgggatccga gacgaaaagt cagggaatac aatcctggac
     2041 tacctgatcg acgatgggat ttctaaccgg aactttatgc aactgataca cgacgatgca
     2101 ttgtccttta aaaagaaaat ccagaaagcc caaattatag gcgacgaaga caagggcaac
     2161 attaaagaag tggttaaatc attgcctggg agccccgcga tcaagaaggg gatactccaa
     2221 tccatcaaaa ttgtggatga gcttgtcaag gtgatgggag gccgaaagcc ggaaagcatt
     2281 gtggtggaga tggctagaga aaatcagtac accaaccagg ggaagagtaa ttcccagcag
     2341 cggcttaaac ggttggaaaa gagcttgaag gaactggggt caaaaattct gaaggagaac
     2401 atccctgcta aactcagcaa gattgataat aacgccctgc agaacgatag gttgtacctg
     2461 tactacctcc agaatgggaa ggacatgtac actggcgacg atctggatat cgacagactg
     2521 tctaactatg atatcgatca tattatccca caggccttcc ttaaggataa ttccatcgat
     2581 aataaggtgc tcgtcagctc tgcatccaat cggggtaaat ctgacgacgt gcctagtctg
     2641 gaggtcgtta agaaacgcaa aacattttgg taccaactgc ttaagtcaaa acttatcagc
     2701 cagcggaagt tcgataattt gacaaaggcc gaacggggcg gcctcagccc tgaggataaa
     2761 gccggtttca tccagaggca actggtggag actcggcaaa ttacaaaaca tgtagctagg
     2821 ctcttggatg agaaattcaa caacaagaag gatgagaata accgagccgt ccgaaccgtg
     2881 aagatcatta ctttgaagag tacgctggtg agtcagtttc ggaaggactt cgagctgtac
     2941 aaagtgcgag aaataaacga ttttcaccat gcccacgatg cctatctgaa cgccgtggtg
     3001 gcctctgctc tcttgaagaa gtatcccaaa cttgagcccg agtttgtgta cggtgattac
     3061 cccaaataca actcctttcg agaaagaaag agtgctaccg aaaaagtcta cttctattcc
     3121 aacatcatga atattttcaa aaaaagcatc tccctggctg atggtcgagt catagagcgg
     3181 cccctcatcg aagtcaatga ggagacgggc gagagtgtgt ggaacaagga atctgatctg
     3241 gccacggtta gaagagtgct tagctacccg caggtcaacg ttgtgaagaa ggttgaagaa
     3301 caaaatcacg gtctggaccg gggaaagcct aaaggcctgt tcaatgctaa cctgtcatca
     3361 aaaccaaagc ctaacagcaa cgaaaacctt gtcggagcta aggaatacct ggacccgaag
     3421 aagtacggtg gatacgccgg catctctaat tcctttaccg tgcttgtcaa gggaaccatc
     3481 gagaagggcg ccaagaagaa gatcacaaac gtcctggaat ttcaggggat cagcatcctg
     3541 gatcggataa attaccgcaa agacaagctt aacttcctcc tggagaaagg ctacaaggac
     3601 atcgagctca ttatcgaact gccaaaatat agtctgtttg agctttccga cggtagccgg
     3661 cggatgctgg caagcattct ttcaaccaac aataagcggg gggaaatcca taaagggaac
     3721 cagatcttcc ttagtcagaa gttcgtcaag ctcctgtacc atgcaaagcg gatatcaaat
     3781 acaatcaatg agaatcaccg aaagtacgtc gagaatcata aaaaggagtt cgaggaactc
     3841 ttttattaca tcctggaatt caatgaaaac tatgtgggag ccaagaaaaa cggcaaactg
     3901 ctgaactcag ctttccagag ctggcaaaac cattccatcg atgagttgtg tagttctttt
     3961 atcggcccta ccggttccga aaggaaagga ttgttcgaac tcacaagcag gggttctgca
     4021 gctgactttg agtttctggg agtgaagatc ccaagatacc gagattatac accctctagt
     4081 ctgcttaagg atgccaccct gatccaccag tctgtgaccg gcctttatga gacaaggatc
     4141 gacctggcta aacttgggga aggc
//
