LOCUS       sequence-195319-        6101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA tag fused to the N-terminus of RILP for the expression in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Wozniak AL, Long A, Jones-Jamtgaard KN, Weinman SA
  TITLE     Hepatitis C virus promotes virion secretion through cleavage of the 
            Rab7 adaptor protein RILP.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12484-12489. Epub 2016 
            Oct 17.
  PUBMED    27791088
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2016-11-1"; volume: "113(44)"; pages: 
            "12484-12489. Epub 2016 Oct 17"
COMMENT     Sequence Label: pTRE2-Bla(HA–RILP)
FEATURES             Location/Qualifiers
     source          1..6101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..341
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     338..362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             527..553
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          1784..2356
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2488..2507)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2553..2608
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2607..2626)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3006..3023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3177..3765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3257..3276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3936..4727,4728..4796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4559..4578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4797..4901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4969..4987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5006..5335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5186..5321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5248..5267
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5383..5430
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5449..5847
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6005..6086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6042..6061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    join(6101,1..18,19..41,42..60,61..83,84..102,103..125,
                     126..144,145..167,168..186,187..209,210..228,229..251,
                     252..270)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
       61 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      121 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      181 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      241 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
      301 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      361 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      421 gcctccgcgg ccccgaattc gagctcggta cccggggatc ctctagtcag ctgacgcgtg
      481 ctagcgcggc cgcatcgata agcttgtcga cgaattcatg actagttacc catacgatgt
      541 tccagattac gctactagtg agcccaggag ggcggcgccc ggggtgcctg gctgggggtc
      601 tcgggaggcc gcggggtcgg catcggccgc ggagcttgtg taccatctag ccggggccct
      661 gggcactgag ctgcaggatc tggcgcgccg tttcgggccg gaggcggcgg ccgggctggt
      721 gccgctagtg gtgcgggcgc tggagctctt ggaacaggct gccgtggggc ccgccccgga
      781 ctcgctgcag gtgtcggcgc agccggcgga gcaggagctg cggcggctgc gggaggagaa
      841 cgagcgcctc cgcagggagc tgcgcgcggg gccacaggag gagcgcgcgc tgctgcggca
      901 gctcaaggag gtcacggacc gacagcggga cgaactccgg gcgcacaacc gcgacctgcg
      961 gcagcgcggc caggagaccg aggcgttgca ggagcagctg cagcgcctcc tgctggtgaa
     1021 cgctgagctg cggcacaagc tggcggccat gcagacccag ctgcgcgccg cgcaggaccg
     1081 cgagagggag cgccagcagc ctggcgaagc cgcgactccg caggctaaag agcgagcgcg
     1141 ggggcaggcc gggcggcccg ggcaccagca cggacaggag cccgaatggg cgaccgccgg
     1201 cgcaggcgcc ccagggaacc ctgaggaccc ggcggaggcc gcgcagcagc tcgggcgccc
     1261 ctcggaggca gggcagtgcc gcttcagtcg ggaggagttt gagcagatcc ttcaggagcg
     1321 gaatgaactc aaagccaaag tgttcctgct caaggaggaa ctggcctact tccagcggga
     1381 gctgctcaca gaccaccggg tccccggcct tctgctcgag gccatgaagg tggctgtccg
     1441 gaagcagcgg aagaagatca aggccaagat gttagggaca ccagaggaag cagagagcag
     1501 tgaggatgag gctggcccat ggatcctgct ctccgatgac aagggagacc atcccccacc
     1561 cccggagtcc aaaatacaga gtttctttgg cctatggtat cggggtaaag ctgaatcctc
     1621 tgaggatgag accagcagcc ctgcacccag caagctaggg ggagaagagg aggcccaacc
     1681 acagtctcca gctcctgatc cgccctgttc tgccctccac gaacaccttt gtctgggggc
     1741 ctcagccgcc ccagaggcct gatctagagc tgagaacttc agggtgagtt tggggaccct
     1801 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     1861 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
     1921 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
     1981 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     2041 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     2101 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     2161 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     2221 ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     2281 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     2341 cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg
     2401 caaagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     2461 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     2521 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     2581 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2641 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     2701 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     2761 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     2821 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     2881 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     2941 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3001 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     4561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4981 acgaggccct ttcgtcttca ctcgagtgtg tcagttaggg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5161 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5221 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5281 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5341 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     5401 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct
     5461 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     5521 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     5581 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     5641 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     5701 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     5761 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     5821 ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacacgt
     5881 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5941 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6001 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6061 cacaaataaa gcattttttt cactgcctcg agtttaccac t
//
