LOCUS       sequence-19550-e         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF116
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(184..201)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    190..414
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagacc cggggagaag
       61 ccttttcaat gcaaccagtg cggtgcgagc ttcacgcaaa agggcaactt actgcgccac
      121 atcaagctgc ataccggtgg atcctctgca cagtagtaag cttaagttta aaccgctgat
      181 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      241 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      301 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
      361 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
      421 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
      481 taagcgcggc gggtgtgggg gttacgcgca gcgtgaccgc tacacttgcc cgcgccctag
      541 cgcccgctcc tttcgctttc t
//
