LOCUS       sequence-19556-e         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF122
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(232..249)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    238..463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(596..615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggctactaga gaaccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctg gctagcgttt aaacgggccc tctagacccg gggagaagcc ttacaagtgc
      121 gaggagtgcg gaaaggcgtt cacgcagtca tcaaacctga cgaagcacaa gaagatccac
      181 accggtggat cctctgcaca gtagtaagct taagtttaaa ccgctgatca gcctcgactg
      241 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      301 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      361 gtaggtgtca ttctattctg gggggtgggg gtggggcagg acagcaaggg ggaggattgg
      421 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
      481 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
      541 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      601 ttcgctttct tcccttcctt tc
//
