LOCUS       sequence-19631-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Murphy KC, Campellone KG
  TITLE     Lambda Red-mediated recombinogenic engineering of enterohemorrhagic 
            and enteropathogenic E. coli.
  JOURNAL   BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.1186/1471-2199-4-11.
  PUBMED    14672541
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2003-12-13"; volume: "4:11. doi"; pages: " 
            10.1186/1471-2199-4-11"
COMMENT     Sequence Label: pKM208
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(88..93,94..109,110..116)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    124..140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     129..151
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             173..589
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
ORIGIN
        1 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatctccgac atcataacgg
       61 ttctggcaaa tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg
      121 gaattgtgag cggataacaa tttcacacag gaaacagtcg acgcttataa aaatggatat
      181 taatactgaa actgagatca agcaaaagca ttcactaacc ccctttcctg ttttcctaat
      241 cagcccggca tttcgcgggc gatattttca cagctatttc aggagttcag ccatgaacgc
      301 ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcactacc agcagctcgc
      361 ccgtgaagag aaagaggcag aactggcaga cgacatggaa aaaggcctgc cccagcacct
      421 gtttgaatcg ctatgcatcg atcatttgca acgccacggg gccagcaaaa aatccattac
      481 ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg gcagaacaca tccggtacat
      541 ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa acgaatgagt
      601 actgcactcg caacgctggc tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac
      661 ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc cagcgatgcg
      721 cagttcatcg cattactgat cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa
      781 atttacgcct ttt
//
