LOCUS       sequence-19632-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Murphy KC, Campellone KG
  TITLE     Lambda Red-mediated recombinogenic engineering of enterohemorrhagic 
            and enteropathogenic E. coli.
  JOURNAL   BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.1186/1471-2199-4-11.
  PUBMED    14672541
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2003-12-13"; volume: "4:11. doi"; pages: " 
            10.1186/1471-2199-4-11"
COMMENT     Sequence Label: pKM208
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..115
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(149..829)
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
ORIGIN
        1 ggttcattcc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
       61 tttgcaccat tcgatggtgt ccccgaatta gcggccgctc tagaactagt ggatcttgcg
      121 cctacccgga tattatcgtg aggatgcgtc atcgccattg ctccccaaat acaaaaccaa
      181 tttcagccag tgcctcgtcc attttttcga tgaactccgg cacgatctcg tcaaaactcg
      241 ccatgtactt ttcatcccgc tcaatcacga cataatgcag gccttcacgc ttcatacgcg
      301 ggtcatagtt ggcaaagtac caggcatttt ttcgcgtcac ccacatgctg tactgcacct
      361 gggccatgta agctgacttt atggcctcga aaccaccgag ccggaacttc atgaaatccc
      421 gggaggtaaa cgggcatttc agttcaaggc cgttgccgtc actgcataaa ccatcgggag
      481 agcaggcggt acgcatactt tcgtcgcgat agatgatcgg ggattcagta acattcacgc
      541 cggaagtgaa ttcaaacagg gttctggcgt cgttctcgta ctgttttccc caggccagtg
      601 ctttagcgtt aacttccgga gccacaccgg tgcaaacctc agcaagcagg gtgtggaagt
      661 aggacatttt catgtcaggc cacttctttc cggagcgggg ttttgctatc acgttgtgaa
      721 cttctgaagc ggtgatgacg ccgagccgta atttgtgcca cgcatcttcc ccctgttcga
      781 cagctctcac atcgatcccg gtacgctgca ggataatgtc cggtgtcatg ctgccacctt
      841 ctgctctgcg gctttctgtt tcaggaatcc aagagctttt actgcttcgg cctgtgtcag
      901 ttctgacana tgcacnaatg tcccggcgaa atatctgg
//
