LOCUS       sequence-19653-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Wang W, Budhu A, Forgues M, Wang XW
  TITLE     Temporal and spatial control of nucleophosmin by the Ran-Crm1 
            complex in centrosome duplication.
  JOURNAL   Nat Cell Biol. 2005 Aug . 7(8):823-30.
  PUBMED    16041368
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Aug . 7(8):823-30."
COMMENT     Sequence Label: GFP-NPM NESM
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..95
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccgggtctc tggcangacg agctgtcagt ccggactcag atctcgagct caagcttcga
       61 attctgcagt cgacggtacc gcgggcccgg gatccgccgc caccatggac tacaaggatg
      121 acgacgacaa gcatatggaa gattcgatgg acatggacat gagccccctg aggccccaga
      181 actatctttt cggttgtgaa ctaaaggccg acaaagatta tcactttaag gtggataatg
      241 atgaaaatga gcaccagtta tctttaagaa cggtcagttt aggggctggt gcaaaggatg
      301 agttgcacat tgttgaagca gaggcaatga attacgaagg cagtccaatt aaagtaacac
      361 tggcaacttt gaaaatgtct gtacagccaa cggtttccct tgggggcttt gaaataacac
      421 caccagtggt cgcaagggcg aagtgtggtt cagggccagt gcatattagt ggacagcact
      481 tagtagctgt ggaggaagat gcagagtcag aagatgaaga ggaggaggat gtgaaactct
      541 taagtatatc tggaaagcgg tctgcccctg gaggtggtag caaggttcca cagaaaaaag
      601 taaaacttgc tgctgatgaa gatgatgacg atgatgatga agaggatgat gatgaagatg
      661 atgatgatga tgattttgat gatggaggaa gctgaagaaa aagcgccagt gaagaaatct
      721 atacgagata ctccagccaa aatgcacaaa gtcanagngg gggaaatcgc cnnccccnnc
      781 cccccccnnn nnnggnggnc annttcttna naancgnaaa anctctaaac c
//
