LOCUS       sequence-196619-        9717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses WT ScVrg4 in S. cerevisiae cells using Galactose 
            induction, -URA and -Leu selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Parker JL, Newstead S
  TITLE     Structural basis of nucleotide sugar transport across the Golgi 
            membrane.
  JOURNAL   Nature. 2017 Nov 23;551(7681):521-524. doi: 10.1038/nature24464. 
            Epub 2017 Nov 15.
  PUBMED    29143814
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature24464"; journalName: "Nature"; date: "2017-11-23- 
            23"; volume: "551"; issue: "7681"; pages: "521-524"
COMMENT     Sequence Label: pDDGFP-Leu2d-ScVrg4
FEATURES             Location/Qualifiers
     source          1..9717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(125..144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     244..266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(304..322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      363..1705
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    695..742
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1732..1836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1837..1905,1906..2697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2055..2074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2868..3456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3357..3376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3610..3627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3744..3765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3780..3785,3786..3803,3804..3810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3818..3834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3823..3845
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3842..3858
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3842..3858
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3877..3897
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3879..3897
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3921..4362
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3921..4038
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4333..4356
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     4377..4393
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4377..4393
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5412..5432
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5439..6149
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             6150..6173
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(6203..6219)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6204..6220)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      6222..6469
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6238..6256)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6487..6506)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6488..6506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6516..6533)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6516..6532)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6525..6547)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6760..6779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(7180..8274)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     8510..8531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8799..9602)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(join(9603..9717,1..101))
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac
       61 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta tggtgcactc
      121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
      301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagt
      361 atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt
      421 ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga
      481 atgggataat atcacaggag gtactagact acctttcatc ctacataaat agacgcatat
      541 aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca
      601 ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg
      661 cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt
      721 ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca
      781 ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg
      841 cttccacaaa cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat
      901 ataacctacc catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt
      961 tttatgttta tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata
     1021 gagtgaatcg aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa
     1081 aatagaagaa accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc
     1141 tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct
     1201 tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct
     1261 ttttttatgg aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac
     1321 agtgcaaaaa gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc
     1381 taagatgctt tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa
     1441 gtatagattc tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg
     1501 cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa
     1561 atgaagcaca gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa
     1621 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta
     1681 caaaatgaag cacagatgct tcgttcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1801 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1921 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2041 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2221 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     2281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2401 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2521 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2701 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3721 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3781 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     3841 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     3901 caaaagctgg agctctagta cggattagaa gccgccgagc gggtgacagc cctccgaagg
     3961 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
     4021 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
     4081 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
     4141 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
     4201 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
     4261 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
     4321 caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggattcta
     4381 gaactagtgg atcccccatg tctgaattga aaacaggtca tgcaggccat aacccttggg
     4441 cttcagttgc caattccggt ccgatctcta ttttatccta ctgtggttcc tctattttaa
     4501 tgacggtgac taacaagttc gtcgtcaatt tgaaggattt caacatgaac tttgtcatgc
     4561 ttttcgtgca atctttggtt tgtactataa ccttgattat cctacgtata ctgggctatg
     4621 cgaagttccg ttcattaaac aaaacagacg ccaagaactg gttccctatt tcctttttac
     4681 tggtcttgat gatctacacc tcttcgaagg ctttacaata cttggctgtt ccaatttaca
     4741 ccattttcaa gaatttgact attatcttga ttgcttatgg tgaggttctc ttttttggtg
     4801 gctctgtcac ctccatggaa ttgtcatcat ttttgttgat ggtcctttct tctgtcgttg
     4861 caacttgggg tgaccagcaa gctgtggctg ccaaggctgc ttcattggct gaaggagcag
     4921 ccggtgctgt tgcctccttt aacccaggtt atttctggat gttcaccaac tgtatcactt
     4981 ctgcattatt cgttcttata atgagaaaga gaattaagtt aactaacttc aaggatttcg
     5041 acactatgtt ttacaacaat gttttggctc tacctattct attgctgttt tctttctgtg
     5101 tggaagattg gtcttcagtt aatttgacca ataacttttc taacgattcg ctaactgcta
     5161 tgatcatcag tggtgttgca tccgtcggta tttcttactg ttccggttgg tgtgttcgtg
     5221 ttacttcgtc tactacatat tcgatggtag gggctttgaa caagctgcca attgccttgt
     5281 ctggtttgat tttctttgat gctccaagaa acttcttatc tattctctcc atttttattg
     5341 gtttcctatc aggtattatt tatgctgttg ccaaacaaaa gaagcaacaa gcccaacctt
     5401 tacgtaaagg ggaaaattta tattttcaag gtcaattttc taaaggtgaa gaattattca
     5461 ctggcgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg
     5521 tctccggtga aggtgaaggt gacgctactt acggtaaatt gaccttaaaa tttatttgta
     5581 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcggt tatggtgttc
     5641 aatgttttgc gagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc
     5701 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca
     5761 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg
     5821 attttaaaga agatggtaac attttaggtc acaaattgga atacaactat aactctcaca
     5881 atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc aaaattagac
     5941 acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat actccaattg
     6001 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca
     6061 aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta
     6121 ttacccatgg tatggatgaa ttgtacaaac accaccacca tcatcatcat cattaactgc
     6181 aggaattcga tatcaagctt atcgataccg tcgacctcga gtcatgtaat tagttatgtc
     6241 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     6301 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     6361 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
     6421 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc
     6481 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     6541 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6601 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6661 aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6781 cctttctcgc cacgttcgcc ggcaaatggt caggtcattg agtgtttttt atttgttgta
     6841 tttttttttt tttagagaaa atcctccaat atataaatta ggaatcatag tttcatgatt
     6901 ttctgttaca cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt
     6961 gcaattcttt ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt
     7021 tacatcctcc tttttctcct tcttgataaa tgtatgtaga ttgcgtatat agtttcgtct
     7081 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     7141 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac
     7201 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt
     7261 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg
     7321 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac
     7381 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt
     7441 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat
     7501 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt
     7561 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac
     7621 cttcaatgta gggaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga
     7681 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag
     7741 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact
     7801 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac
     7861 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa
     7921 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc
     7981 tttacggatt tttagtaaac cttgttcagg tctaacacta ccggtacccc atttaggacc
     8041 acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg
     8101 aagtggaaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa
     8161 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat
     8221 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattacaat
     8281 ggtatatcct tgaaatatat ataaaaaaag gcgccttaga ccgctcggcc aaacaacgcc
     8341 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8401 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8461 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8521 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8581 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8641 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     8701 gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt
     8761 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     8821 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     8881 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     8941 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     9001 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     9061 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     9121 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     9181 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     9241 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     9301 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     9361 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     9421 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     9481 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     9541 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     9601 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
     9661 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgc
//
