LOCUS       sequence-196858-         452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP002
FEATURES             Location/Qualifiers
     source          1..452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg
       61 agctctttgc acttctttag ttctttccat caatatcagt tttttaaact tttaggacta
      121 aaagtgatgt ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat
      181 actaatacac tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa
      241 aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt
      301 agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac
      361 atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca
      421 tgtatataaa cgtcaaatca taatcagcac ta
//
