LOCUS       sequence-196983-        7114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives expression of rpaA from the NS1 site in the S. elongatus 
            PCC7942 genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: rpaA_NS1
FEATURES             Location/Qualifiers
     source          1..7114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     86..108
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             3255..4046
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6200..6788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6689..6708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6942..6959
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6974..7114
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7040..7059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       61 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
      121 tcaccgaaac gcgcgaggca ggatcgatcc ggcagccggc ggagcgctgc tttcttggca
      181 agcggtcgcc agccccaacg ccagggctgc cagcccgaaa cagcggggca aggcagcttg
      241 gaagggcgat cgcagcacgg gcatggcaat gtctctctga aggaatgcag accttattcg
      301 tacagccagg gttgaatcgt gggggtccaa tcacttagct ctgctgggct aaaccagaga
      361 gcaatttcct gttgtgctgt ttcgattgca tccgagccat ggatgatgtt gcggccaata
      421 ttgacaccaa aatcaccacg gatggtgccc ggttctgccg tcagcggatt ggtagcgccg
      481 atcaacttgc gagcagccgc cacaacgcct tcgccttcca agacgatcgc cacgatcggc
      541 ccagaggtga tgaactcgac gaggccattg aagaaggggc gctcgcggtg gacagcatag
      601 tgctgttcgg ccagctcgcg actgggcttc agctgcttta ggcccaccag tttgaagcct
      661 ttttgctcaa agcggccgat gatcgtaccg accaaacccc gctgaacgcc atcgggcttg
      721 atggcaataa atgtgcgttc cacagacatc tagatagtcc tcaagacgag gcaagcattg
      781 agcttgcctt cctatggttc gggatcactg ggattcttga caagcgatcg cggtcacatc
      841 gctatctctt aggacttcgc agcgggcgag tcggattgac ccggtaggga tttcgccaga
      901 tcaatgcccg tggtttgttt cagcttctcc agcaagctag cgatttgggt agcgctgcct
      961 tccccttcgc caatcacagt gatcgactcc acgtcgatat ctggcacggt gcctgaaagc
     1021 gtgacgagca gggactcgaa ttacttgagt tgatcaaggc tcagatcaag agcgatcgct
     1081 acttcaatac agcttacttt tggccgctat ctcaacagca gttcaatcct aagaaactgc
     1141 agaagcaggg attgagtaat ttgatggttg actgcagtcc ttatcagttg cttctcaaca
     1201 attatccaac cgcaattacg gacagagcac tggactagca ccactgctaa aaccgcaaag
     1261 ctcggacaaa tataaagaca acttaaagat tttctacgcc ctgcgacctc accagaacag
     1321 agccactatc atttttaaga aaacataaat tgatccgctg taagccgctg ccagcacaaa
     1381 gcttaccgcg tttttgctaa agcctagtcc gctggtttgt cctcccggaa tgttacccta
     1441 tgaaaccccg catcctcgtg atcgatgatg actcagccat cttggagctg gtcgccgtca
     1501 atctggagat gtctggctat gacgtacgca aagctgagga cggcattaaa ggtcaggctt
     1561 tagctgttca gctagttccc gacctgatca tgctggatct aatgctgccg cgggttgatg
     1621 gctttaccgt ctgtcagcga ctgcggcgcg atgagcgtac tgccgaaatt ccggtgctga
     1681 tgctgaccgc cctcggacag actcaggata aggttgaagg cttcaacgcg ggtgctgacg
     1741 attatctgac taagcccttc gaagttgaag agatgctggc ccgcgtgcgt gccttgctgc
     1801 ggcgcaccga tcgcattccc catgcagccc gccatagcga aattctcagc tacggtccgc
     1861 tgaccctgat tcccgagcgg tttgaggcca tttggttcaa ccgcacggtc aagctgactc
     1921 acttggaatt tgagttgttg cactgcctgt tgcaacgcca cggccaaacg gttgcgccga
     1981 gcgaaatcct caaagaagtc tggggctatg atcccgacga tgacatcgag acgattcgcg
     2041 tccacatccg tcatctgcgc accaagctcg agcccgatcc ccggcacccg cgctacatca
     2101 aaacggtcta tggagcgggc tactgccttg agctgccggc cgagacggaa ctccaccaac
     2161 acgccgatca gtttccttcg gcgtcctgaa cccaagcact gagcagcact tcccagacga
     2221 gtcgcggctg aacaaaggct cgcagttgtt tctgtgcctg ttcgagctgg agcagttgct
     2281 gggactgcag ctgttgctgg gaccagccct gctgctggag gtagcgacaa agccaaagct
     2341 gggcttcgac gtcgaggcca gcatcgatcg cttgcgccat gcatgcctgc aggtcgactc
     2401 tagagcttta tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct
     2461 ctagggtccc caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac
     2521 cggatcaatt cccctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc
     2581 gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct
     2641 tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga
     2701 acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca
     2761 ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg
     2821 tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga
     2881 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca
     2941 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     3001 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     3061 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     3121 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3181 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3241 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3301 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3361 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3421 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3481 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3541 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3601 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3661 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3721 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3781 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3841 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3901 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3961 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     4021 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg
     4081 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     4141 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     4201 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     4261 acatccgcat taaaatctag cgagggcttt actaagctga tccggtggat gaccttttga
     4321 atgaccttta atagattata ttactaatta attggggacc ctagaggtcc ccttttttat
     4381 tttaaaaatt ttttcacaaa acggtttaca agcatactag aggatcggcg gccgcggatc
     4441 cccgggtacc gagctcgaat ttcgagcttc tggagcagga agatgtcgcg ggcattagca
     4501 ccagcggtct gccaagcctc cgccagccgt tgggtccctt ccgcttgagc ttttccatct
     4561 tcgacgatac gggcggcggc cccccgcgct tccgcgatcg cccgtttaca agctgcctca
     4621 gctggggcga tcacatcggc ttgaagttgc tgctgcacct gtttgatccg ctcctgctgc
     4681 acagggagtt ctgcttggct acgagcgact tcggtagcaa tgtccgcttc agcttcggcc
     4741 accaccgctt cgcgccgcgt caacgcatcc tgaatccggc gctcggcctc ggcttgggcg
     4801 atcgctacat cgcgatcgat ccgacgcagg gccgtgatct tgtcattttc ggccgtttgg
     4861 atcgcagagg cagcctgggc atcggcttca gcaattcggg catctcgctg cagatcagcc
     4921 cgctgcttgc gtccactagc cgagagataa ccgacctcat cggaaatgtt ctggacttgc
     4981 agcgtatcga ggactagacc cagctgctca aggtcatcct ccgcctcttc cagcagactt
     5041 ttggcaaagg caattttgtc ctcgttgatc tgctccggcg tgaggctggc taaaacacca
     5101 cgcaagttgc cttcgagggt ctccttggca atttgctcga tttccttacg gtttttgcca
     5161 agcagccgct cgatcgcgtt gtggatggtc ggttcttccc cagcaatctt gatattggca
     5221 acgccttcaa cagtcagggg aatgccgccc ttggagaagg cattggaaac gcgcaactca
     5281 atgatcatgt tggtcagatc catgcggagc gctttttcca gcagaggtac ccgcaggctg
     5341 ctgccgccct tgaccaagcg atagccaact cggcggccat cactactgcg gcgactactg
     5401 ccagcaaaga tcaaaatttc actgggttgg cagatgtagt agagattgcg caggactaag
     5461 ctgccagccc cggcgactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5521 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5581 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5641 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5701 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5761 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5821 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5881 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5941 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6001 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6061 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6121 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6841 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6901 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6961 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7021 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     7081 tccgctatcg ctacgtgact gggtcatggc tgcg
//
