LOCUS       sequence-197027-        8409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives expression of galP transporter from NS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::galP_NS1
FEATURES             Location/Qualifiers
     source          1..8409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1315..1392
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1393..2475
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1412..1431)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(2707..2712,2713..2729,2730..2736)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2744..2760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2749..2771
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      7495..8083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7984..8003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8237..8254
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8269..8409
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8335..8354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       61 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
      121 tcaccgaaac gcgcgaggca ggatcgatcc ggcagccggc ggagcgctgc tttcttggca
      181 agcggtcgcc agccccaacg ccagggctgc cagcccgaaa cagcggggca aggcagcttg
      241 gaagggcgat cgcagcacgg gcatggcaat gtctctctga aggaatgcag accttattcg
      301 tacagccagg gttgaatcgt gggggtccaa tcacttagct ctgctgggct aaaccagaga
      361 gcaatttcct gttgtgctgt ttcgattgca tccgagccat ggatgatgtt gcggccaata
      421 ttgacaccaa aatcaccacg gatggtgccc ggttctgccg tcagcggatt ggtagcgccg
      481 atcaacttgc gagcagccgc cacaacgcct tcgccttcca agacgatcgc cacgatcggc
      541 ccagaggtga tgaactcgac gaggccattg aagaaggggc gctcgcggtg gacagcatag
      601 tgctgttcgg ccagctcgcg actgggcttc agctgcttta ggcccaccag tttgaagcct
      661 ttttgctcaa agcggccgat gatcgtaccg accaaacccc gctgaacgcc atcgggcttg
      721 atggcaataa atgtgcgttc cacagacatc tagatagtcc tcaagacgag gcaagcattg
      781 agcttgcctt cctatggttc gggatcactg ggattcttga caagcgatcg cggtcacatc
      841 gctatctctt aggacttcgc agcgggcgag tcggattgac ccggtaggga tttcgccaga
      901 tcaatgcccg tggtttgttt cagcttctcc agcaagctag cgatttgggt agcgctgcct
      961 tccccttcgc caatcacagt gatcgactcc acgtcgatat ctggcacggt gcctgaaagc
     1021 gtgacgagca gggactcgat gctagctcga gcccgcgaca tcttcctgct ccagaagctc
     1081 gaaattcgag ctcggtaccc ggggatctgg gccgcgggtc atggctgcgc cccgacaccc
     1141 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     1201 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     1261 cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcattta cgttgacacc
     1321 atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
     1381 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
     1441 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
     1501 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
     1561 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
     1621 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
     1681 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
     1741 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
     1801 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
     1861 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
     1921 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
     1981 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
     2041 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
     2101 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
     2161 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
     2221 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
     2281 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     2341 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     2401 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2461 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag cgcgaattga tctggtttga
     2521 cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc
     2581 tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc
     2641 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat
     2701 gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag cggataacaa
     2761 tttcacacag gaaacagacc atggaattca tgcctgacgc taaaaaacag gggcggtcaa
     2821 acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta ctctttggcc
     2881 tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc cagattactt
     2941 cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg
     3001 gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc ggcgcaattt
     3061 tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt
     3121 cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg ctgtacctct
     3181 ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag ttgatgatca
     3241 ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc ggtgcatggc
     3301 gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt gtcttcttcc
     3361 tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc gaacgcgtgc
     3421 tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa atccgtgaaa
     3481 gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac ttccgccgcg
     3541 cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg aacgtcatca
     3601 tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacactacc gagcaaatgt
     3661 gggggaccgt gattgtcggc ctgaccaacg tacttgccac ctttatcgca atcggccttg
     3721 ttgaccgctg gggacgtaaa ccaacgctaa cgctgggctt cctggcgatg gctgctggca
     3781 tgggcgtact cggtacaatg atgcatatcg gtattcactc tccgtcggcg cagtatttcg
     3841 ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt ccgctgattt
     3901 gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc acctgctcca
     3961 ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc atgctcaaca
     4021 cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg tttatcctgc
     4081 tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt gaacgtaatc
     4141 tgatgaaagg tcgtaaactg cgcgaaatag gcgctcacga ttaatgcatg cctgcaggtc
     4201 gactctagag ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg
     4261 gacctctagg gtccccaatt aattagtaat ataatctatt aaaggtcatt caaaaggtca
     4321 tccaccggat caattcccct gctcgcgcag gctgggtgcc aagctctcgg gtaaatccag
     4381 agctcatccg gcggcggcgg cgggataccg gtttcgaaga attctcatgt ttgacagctt
     4441 atcatcgaat tataggaata gagcaaacaa gcaaaggaaa ttttgtcaaa ataattttat
     4501 tgacaacgtc ttattaacgt tgatataatt taaattttat ttgacaaaaa tgggctcgtg
     4561 ttgtacaata aatgtagtga ggtggatgca atggcgaaga cgttgtccga tattaaaaga
     4621 tcgcttgatg ggaatttagg taaaaggctg acgttaaaag caaacggtgg ccggatccat
     4681 atgaccaccc tggatgatac cgcctaccgc taccgcacca gcgttcccgg tgatgccgaa
     4741 gccatcgaag ccctggatgg cagctttacc accgataccg tgtttcgcgt gaccgccacg
     4801 ggtgatggct ttaccctgcg cgaagtgccc gtcgatcccc ctctgaccaa tgtgtttccc
     4861 gatgatgaaa gtgatgatga atcggatgct ggcgaagatg gcgatcccga tagccgcacc
     4921 tttgtggcct acggtgatga tggcgatctg gctggctttg tggtggtgag ctacagcggc
     4981 tggaatcgcc gcttgaccgt ggaagatatt gaagtggctc ccgaacaccg cggtcacggc
     5041 gttggtcgcg ctctgatggg cctggccacc gaatttgctc gcgaacgcgg tgctggccac
     5101 ctgtggctgg aagtgaccaa cgtgaacgct cccgctatcc acgcctatcg ccgcatgggc
     5161 ttcaccctgt gtggcctgga taccgctctg tacgatggca ccgccagtga tggcgaacag
     5221 gccctgtaca tgagcatgcc ctgcccctaa ggccggccag cccgcctaat gagcgggctt
     5281 ttttttggcg cgccgctagc gagctcgact aggcgagatc accaaggtag tcggcaaata
     5341 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     5401 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     5461 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt
     5521 ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa tctagcgagg
     5581 gctttactaa gctgatccgg tggatgacct tttgaatgac ctttaataga ttatattact
     5641 aattaattgg ggaccctaga ggtccccttt tttattttaa aaattttttc acaaaacggt
     5701 ttacaagcat actagaggat cggcggccgc ggatccccgg gtaccgagct cgaatttcga
     5761 gcttctggag caggaagatg tcgcgggcat tagcaccagc ggtctgccaa gcctccgcca
     5821 gccgttgggt cccttccgct tgagcttttc catcttcgac gatacgggcg gcggcccccc
     5881 gcgcttccgc gatcgcccgt ttacaagctg cctcagctgg ggcgatcaca tcggcttgaa
     5941 gttgctgctg cacctgtttg atccgctcct gctgcacagg gagttctgct tggctacgag
     6001 cgacttcggt agcaatgtcc gcttcagctt cggccaccac cgcttcgcgc cgcgtcaacg
     6061 catcctgaat ccggcgctcg gcctcggctt gggcgatcgc tacatcgcga tcgatccgac
     6121 gcagggccgt gatcttgtca ttttcggccg tttggatcgc agaggcagcc tgggcatcgg
     6181 cttcagcaat tcgggcatct cgctgcagat cagcccgctg cttgcgtcca ctagccgaga
     6241 gataaccgac ctcatcggaa atgttctgga cttgcagcgt atcgaggact agacccagct
     6301 gctcaaggtc atcctccgcc tcttccagca gacttttggc aaaggcaatt ttgtcctcgt
     6361 tgatctgctc cggcgtgagg ctggctaaaa caccacgcaa gttgccttcg agggtctcct
     6421 tggcaatttg ctcgatttcc ttacggtttt tgccaagcag ccgctcgatc gcgttgtgga
     6481 tggtcggttc ttccccagca atcttgatat tggcaacgcc ttcaacagtc aggggaatgc
     6541 cgcccttgga gaaggcattg gaaacgcgca actcaatgat catgttggtc agatccatgc
     6601 ggagcgcttt ttccagcaga ggtacccgca ggctgctgcc gcccttgacc aagcgatagc
     6661 caactcggcg gccatcacta ctgcggcgac tactgccagc aaagatcaaa atttcactgg
     6721 gttggcagat gtagtagaga ttgcgcagga ctaagctgcc agccccggcg actcaccagt
     6781 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6841 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6901 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6961 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     7021 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7081 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7141 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7201 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7261 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7321 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7381 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7441 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7501 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7561 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7621 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7681 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7741 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7801 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7861 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7921 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7981 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8041 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8101 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8161 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8221 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8281 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     8401 atggctgcg
//
