LOCUS       sequence-197163-       10272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, 
            Iacobuzio-Donahue CA, Massague J
  TITLE     TGF-beta Tumor Suppression through a Lethal EMT.
  JOURNAL   Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. 
            Epub 2016 Feb 18.
  PUBMED    26898331
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.009"; journalName: "Cell"; date: 
            "2016-02-25- 25"; volume: "164"; issue: "5"; pages: "1015-30"
COMMENT     Sequence Label: GEPIR Sox4.2137
FEATURES             Location/Qualifiers
     source          1..10272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      30..618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     519..538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     772..789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    906..927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(942..947,948..965,966..972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    980..996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     985..1007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1004..1020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1004..1020
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1039..1059
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1041..1059
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1087..1313
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1314..1494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1541..1666
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2159..2392
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2577..2621
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2888..3005
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3021..3399
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3029..3047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3065..3083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3101..3119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3137..3155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3173..3191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3209..3227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3245..3263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3308..3332
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3423..3425,3426..3428,3429..4142)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3468..3489)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3729..3748)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4076..4097
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           4336..4350
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        4529..5028
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4564..4587)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4961..4980
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5049..5648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5049..5068)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5545..5565
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5709..6276
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5726..6278
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5870..5887)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6097..6116
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6290..6907,6908..6913,6914..6949,6950..6952,6953..6988,
                     6989..6991,6992..7027,7028..7036)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7082..7670
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7135..7155)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7553..7564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7877..8110
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8182..8303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8219..8238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8273..8292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8343..8478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8498..8517)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8499..8517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8527..8544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8527..8543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8536..8558)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8685..9140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8772..8791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8982..9003
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9166..9270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9271..9339,9340..10131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9489..9508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      781 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      841 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      901 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      961 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     1021 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     1081 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     1141 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     1201 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     1261 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     1321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     1381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     1441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1501 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1561 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1621 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1681 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1741 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1801 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1861 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1921 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1981 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     2041 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2101 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2161 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2221 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2281 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2341 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2401 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     2461 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2521 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2581 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2641 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2701 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2761 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2821 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     2881 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2941 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     3001 attttatcga ttctagattc gagtttactc cctatcagtg atagagaacg tatgaagagt
     3061 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     3121 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     3181 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     3241 ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata
     3301 aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt
     3361 cttataccaa ctttccgtac cacttcctac cctcgtaaag tcgagcttgc gttggatcca
     3421 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3481 acggcgacgt aaacggccac aagttcagcg tgtctggcga gggcgagggc gatgccacct
     3541 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3601 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3661 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3721 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3781 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3841 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3901 acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3961 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4021 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4081 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4141 aatgacgtac ggcggccgcc tcgactaggg ataacagggt aattgtttga atgaggcttc
     4201 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcgact
     4261 tcttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gctagatgga
     4321 gagtagaagg agatagtgaa gccacagatg tatctccttc tactctccat ctattgccta
     4381 ctgcctcgga cttcaagggg ctagaattcg agcaattatc ttgtttacta aaactgaata
     4441 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
     4501 cttttttcaa ttgacgcgta attctaccgg gtaggggagg cgcttttccc aaggcagtct
     4561 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     4621 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     4681 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     4741 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     4801 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     4861 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     4921 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     4981 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     5041 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5101 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5161 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5221 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5281 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5341 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5401 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5461 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5521 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5581 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5641 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     5701 catccaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5761 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5821 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5881 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5941 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6001 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6061 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6121 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6181 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6241 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tgagtagact
     6301 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
     6361 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     6421 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     6481 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     6541 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     6601 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     6661 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     6721 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     6781 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     6841 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     6901 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
     6961 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
     7021 catgctcccc gggtaatccg gactcgacct gcagccaagc ttatcgaaac gcgtctggaa
     7081 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7141 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7201 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7261 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7321 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7381 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7441 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7501 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7561 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7621 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     7681 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7741 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7801 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7861 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7921 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7981 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8041 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8101 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     8161 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     8221 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8281 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     8341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8461 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     8521 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8581 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8641 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     8701 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8761 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8821 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8881 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8941 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9001 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9061 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     9121 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9181 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9241 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9301 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     9361 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9421 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9481 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     9541 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9601 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9661 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9721 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9781 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9841 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9901 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9961 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10021 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10081 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10141 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10201 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10261 cactgagcgt ca
//
